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MAB5234 | Anti-Neurocan Antibody

50 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ch ELISA, IP, WB M Purified Monoclonal Antibody
      Catalogue NumberMAB5234
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Neurocan Antibody
      Background InformationNeurocan is the major soluble chondroitin sulfate proteoglycan in the brain. It is thought to play a functional role in axonal growth and guidance and in the establishment of specific neural pathways during embryonic brain development. Neurocan expression in the brain is developmentally regulated. Early on the major form of neurocan consists of a 245 kD core protein with approximately two chondroitin sulfate glycosoaminoglycan chains of 22 kD each. Later neurocan comprises a 180 kD core protein. Both forms of neurocan contain only chondroitin 4-sulfate glycosoaminoglycan chains. By virtue of their high expression at sites of neurnal damage and trauma, chondroitin sulfate proteoglycans, including neurocan, are thought to inhibit successful nerve regeneration.
      Product Information
      PresentationPurified immunoglobulin. Liquid in PBS. Contains no preservative.
      ApplicationAnti-Neurocan Antibody is an antibody against Neurocan for use in ELISA, IP & WB.
      Key Applications
      • ELISA
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern blot: < 0.5 μg/mL

      Western blot using anti-chick Neurocan (MAB5234). Samples are 1) Untreated embryonic chick brain extract, 2) chondroitinase-treated embryonic chick brain extract, 3) GST fusion proteins from the middle region of chick neurocan.

      Samples must be digested with chondroitinase prior to running on SDS gels because undigested phosphacan is too large for most gels. Treatment is at a concentration of chondroitinase of 10U/mL in Tris-HCL pH 8.0. Make tissue or cell extract in 20-50mM Tris pH 7.6-8.0 with 0.15M NaCl in the presence of protease inhibitors. Add 1 microliter of enzyme to 30 microliters of extract and incubate 30 minutes at 37C. Then add SDS sample buffer, heat or boil sample as normal for SDS reducing samples.

      Immunoprecipitation: 1 μg/mL

      ELISA: 1 μg/mL, excellent for core protein, good for monomer

      Immunocytochemistry: not tested

      Immunohistochemistry: does not work on fixed samples, unfixed has not been tested.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenMixture of chicken chondroitin sulfate proteoglycans.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityNeurocan, chicken. Recognizes the middle region of neurocan.
      Species Reactivity
      • Chicken
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryNeurocan is a chondroitin sulfate proteoglycan thought to be involved in the modulation of cell adhesion and migration.[supplied by OMIM]
      Gene Symbol
      • NCAN
      • FLJ44681
      • NEUR
      • CSPG3
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O14594 # May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.
      SIZE: 1321 amino acids; 143093 Da
      SIMILARITY: SwissProt: O14594 ## Belongs to the aggrecan/versican proteoglycan family. & Contains 1 C-type lectin domain. & Contains 2 EGF-like domains. & Contains 1 Ig-like V-type (immunoglobulin-like) domain. & Contains 2 Link domains. & Contains 1 Sushi (CCP/SCR) domain.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Polysialic Acid-NCAM, clone 2-2B - NMM1745347 NMM1745347
      MOUSE ANTI-NEUROCAN (CHICKEN) -2622639 2622639
      MOUSE ANTI-NEUROCAN (CHICKEN) -2638922 2638922
      MOUSE ANTI-NEUROCAN (CHICKEN) -2662389 2662389
      MOUSE ANTI-NEUROCAN (CHICKEN) -2818218 2818218


      Reference overviewPub Med ID
      Polysialylated NCAM and ephrinA/EphA regulate synaptic development of GABAergic interneurons in prefrontal cortex.
      Brennaman, LH; Zhang, X; Guan, H; Triplett, JW; Brown, A; Demyanenko, GP; Manis, PB; Landmesser, L; Maness, PF
      Cerebral cortex (New York, N.Y. : 1991) 23 162-77 2013

      Show Abstract
      22275477 22275477

      Data Sheet

      Anti-Neurocan - Data Sheet

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      Life Science Research > Antibodies and Assays > Primary Antibodies