MABD401 | Anti-Neural Stem Cells Antibody, clone Nilo1

NewMABD401
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M FC, ICC, IHC, IF, IP, Inhibition, Magnetic Resonance Imaging, WB AHm Purified Monoclonal Antibody
      Description
      Catalogue NumberMABD401
      DescriptionAnti-Neural Stem Cells Antibody, clone Nilo1
      Alternate Names
      • Neural stem cells
      • Neural progenitors
      • NSCs
      • Type B astrocytes
      Background InformationSimilar to other cell types, neural cells are generated from primary progenitor cells known as type B astrocytes or neural stem cells (NSCs) via stages of amplification (transient amplifying cells), resulting in the generation of intermediate precursor cells (iPCs) committed to neurons (nIPCs), astrocytes (aIPCs), or oligodendrocytes (oIPCs). In adult rodent brain, NSCs and iPCs are mainly restricted to niches in the subventricular zone (SVZ) of the lateral ventricles (LV). In the adult SVZ, type B cells (SVZB) generate neuroblasts (type A cells, neuronal precursors) through a highly proliferative transit amplifying population (type C cells). NSCs and iPCs paly important roles in neurogenesis in the adult brain. Neuroblasts, for example, are known to migrate from their SVZ niche through the rostral migratory stream (RMS) to the olfactory bulb for the continuous generation of periglomerular interneurons. In addition to maintaining the homeostasis of the olfactory bulb, both neuroblasts and NSCs are shown to mobilize from their SVZ niches to various brain damage sites caused by tumor growth, cryolesion, focal demyelinization, and mechanical lesion.
      References
      Product Information
      FormatPurified
      PresentationPurified Armenian hamster monoclonal antibody in PBS without preservatives.
      Applications
      ApplicationThis hamster monoclonal Anti-Neural Stem Cells Antibody, clone Nilo1, Cat. No. MABD401, is validated for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Magnetic Resonance Imaging, Inhibition, and Western Blotting.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunofluorescence
      • Immunoprecipitation
      • Inhibition
      • Magnetic Resonance Imaging
      • Western Blotting
      Application NotesMagnetic Resonance Imaging (MRI) Analysis: A representative lot, when coupled to magnetic glyconanoparticles (mGNPs) and injected in mice, allowed the tracking of neural stem cells migrating from their niches towards brain injury sites by MRI (Elvira, G., et al. (2015). Stem Cell Res. 14(1):114-129).

      Flow Cytometry Analysis: A representative lot, coupled to magnetic glyconanoparticles (mGNPs) or not, immunostained the surface of SVZ neurosphere-derived single cells (Elvira, G., et al. (2015). Stem Cell Res. 14(1):114-129).

      Flow Cytometry Analysis: Clone Nilo1 hybridoma culture supernatant stained the surface of 61% and 71% neurosphere-derived cells from mouse embryo olfactory bulb (OB) and adult mouse subventricular zone (SVZ), respectively (Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).

      Immunocytochemistry Analysis: A representative lot immunostained 4% paraformaldehyde-fixed primary human glioblastoma grown in culture as neurospheres (Elvira, G., et al. (2015). Stem Cell Res. 14(1):114-129).

      Immunocytochemistry Analysis: Representative lots immunostained early neural precursor cells by fluorescent immunocytochemistry staining of cultured neurospheres or neurosphere-derived single cells fixed with 4% paraformaldehyde. A loss of Nilo1 immunoreactivity was observed within 7 days following differentiation induction by EGF and bFGF withdrawal in neurosphere cultures (Elvira, G., et al. (2015). Stem Cell Res. 14(1):114-129; Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).

      Immunofluorescence Analysis: A representative lot, when injected in mice, allowed the labelling and detection of undifferentiated neural stem cells migrating towards brain injury sites due to CT-2A tumor growth, cryolesion, demyelination, or mechanical damage via stereotaxic PBS injection (Elvira, G., et al. (2015). Stem Cell Res. 14(1):114-129).

      Immunofluorescence Analysis: Representative lots immunostained quiescent neural progenitor cells, whereas Nilo2 (Cat. No. MABD402) stained post-mitotic neuronal precursors (e.g. type 1 neuroblasts) in subventricular zone (SVZ) by fluorescent immunohistochemistry using 4% paraformaldehyde-fixed adult mouse floating sections (Elvira, G., et al. (2015). Stem Cell Res. 14(1):114-129; Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).

      Immunohistochemistry Analysis: A representative lot immunostained a small cell population lining the SVZ ventricle, distinct from the thin layer of cells stained by Nilo2 (Cat. No. MABD402) in the periventricular area inside the anterior SVZ (SVZa), at the beginning of the RMS. Both Nilo1 and Nilo2 stained ependymal and subependymal layers in adult mouse olfactory bulb core, neither clone stained dentate gyrus (DG) of the hippocampus (Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated a ~40 kDa glycoprotein from neurosphere cell membrane extracts and from SVZ neurosphere cell lysates (Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).

      Inhibition Analysis: A representative lot inhibited the differentiation of adult mice SVZ-derived neurospheres in culture as well as the proliferation of neurosphere-derived single cells (Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).

      Western Blotting Analysis: A representative lot detected a ~40 kDa glycoprotein in the immunoprecipitates obtained by clone Nilo1 from neurosphere cell membrane extracts and from SVZ neurosphere cell lysates (Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).
      Biological Information
      ImmunogenE13.5 FVB mouse embryo olfactory bulb-derived neurospheres (Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).
      CloneNilo1
      ConcentrationPlease refer to lot specific datasheet.
      HostArmenian Hamster
      SpecificityNilo (Neural Identification Lineage from Olfactory bulb) monoclonal antibody Nilo1 (Cat. No. MABD401) recognizes neural stem cells (NSCs; type B astrocytes) in adult mouse brain and radial glia in mouse embryo (Elvira, G., et al. (2015). Stem Cell Res. 14(1):114-129), while Nilo2 (Cat. No. MABD402) targets neuroblasts (Elvira, G., et al. (2012). PLoS One. 7(9):e44466). Clone Nilo1 identified early progenitor cells (Sox2+, EGFR+, GFAP+ and vimentin+), whereas Nilo2 identified more differentiated neural progenitor cells committed to the neuroblast pathway (Tuj-1+, PSA-NCAM+ and DCX+). Nilo1 and Nilo2 arrest neurosphere proliferation in vitro and interfered with their differentiation into mature neural cells by targeting different surface glycoproteins highly relevant on neural stem/early progenitor cell biology (Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified.
      Molecular Weight~40 kDa (glycosylated) reported. (Del Valle, I., et al. (2010). Neuroscience. 169(3):1473-1485).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in C57BL/6 mouse brain sections.

      Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody immunostained neural stem cells in subventricular zone (SVZ) of the lateral ventricle (LV) in 4% paraformaldehyde-fixed free-floating C57BL/6 mouse brain sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications