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MAB5272 | Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324

MAB5272
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R ICC, IHC, WB R Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB5272
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Neural Cell Adhesion Molecule L1 Antibody, clone 324
      Alternate Names
      • CD171
      • N-CAM L1
      Background InformationFamilies of adhesion molecules which share common carbohydrate domains do exist, despite the structural and functional diversity of these glycoproteins. These include the Ca2+-independent neural adhesion molecules: N-CAM, myelin associated glycoprotein (MAG) and L1. L1 is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts
      References
      Product Information
      FormatPurified
      Control
      • Brain tissue
      PresentationRat monoclonal in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
      Applications
      ApplicationThis Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324 is validated for use in IC, IH, WB for the detection of Neural Cell Adhesion Molecule L1.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern blotting:
      20 μg/mL was used on a previous lot.

      Immunocytochemistry:
      5-10 μg/mL was used on a previous lot. Cells must be fixed with methanol for 10 minutes at -20°C in the middle log phase.

      Immunohistochemistry: Clone 324 is sensitive to fixation. Fresh frozen, acetone or -20C methanol fixed tissues are recommended. Traditional 4% PFA typically does not work well. Other fixatives have not been tested.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRats were immunized with a glycoprotein fraction from cerebellum of 8-10 day old C57BL/6J mice.
      Clone324
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      SpecificityMonoclonal antibody against neural cell adhesion molecule L1 from rat-rat hybrid cells.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene.
      Gene Symbol
      • VCAM1
      • INCAM-100
      • MGC99561
      • CD106
      • DKFZp779G2333
      • L1CAM
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P19320 # Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
      SIZE: 739 amino acids; 81276 Da
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.
      DOMAIN: SwissProt: P19320 Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
      PTM: Sialoglycoprotein.
      DISEASE: SwissProt: P19320 # May play an important role in the genesis of atherosclerosis and rheumatoid arthritis.
      SIMILARITY: Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
      Molecular Weight120-140 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Neural Cell Adhesion Molecule -2649188 2649188
      Anti-Neural Cell Adhesion Molecule -2757143 2757143
      Anti-Neural Cell Adhesion Molecule -2819633 2819633
      Anti-Neural Cell Adhesion Molecule L1, clone 324 2465214
      Anti-Neural Cell Adhesion Molecule L1, clone 324 - 2424730 2424730
      Anti-Neural Cell Adhesion Molecule L1, clone 324 - 2165840 2165840
      Anti-Neural Cell Adhesion Molecule L1, clone 324 - 2189706 2189706
      Anti-Neural Cell Adhesion Molecule L1, clone 324 - LV1640435 LV1640435
      Anti-Neural Cell Adhesion Molecule L1, clone 324 - LV1689948 LV1689948
      Anti-Neural Cell Adhesion Molecule L1, clone 324 - LV1757010 LV1757010
      Anti-Neural Cell Adhesion Molecule L1, clone 324 - LV1780405 LV1780405
      Anti-Neural Cell Adhesion Molecule L1, clone 324 - NG1897864 NG1897864
      Anti-Neural Cell Adhesion Molecule L1, clone 324 -2506457 2506457
      Anti-Neural Cell Adhesion Molecule L1, clone 324 -2572962 2572962
      Anti-Neural Cell Adhesion Molecule L1, clone 324 -2683909 2683909
      Anti-Neural Cell Adhesion Molecule L1, clone 324 -2730953 2730953
      Anti-Neural Cell AdhesionMolecule L1, clone 324 - LV1361634 LV1361634
      Anti-Neural Cell AdhesionMolecule L1, clone 324 - LV1519150 LV1519150

      References | 24 Available | See All References

      Reference overviewPub Med ID
      TUBB5 and its disease-associated mutations influence the terminal differentiation and dendritic spine densities of cerebral cortical neurons.
      Ngo, L; Haas, M; Qu, Z; Li, SS; Zenker, J; Teng, KS; Gunnersen, JM; Breuss, M; Habgood, M; Keays, DA; Heng, JI
      Human molecular genetics 23 5147-58 2014

      Show Abstract
      24833723 24833723
      Unc5C and DCC act downstream of Ctip2 and Satb2 and contribute to corpus callosum formation.
      Srivatsa, S; Parthasarathy, S; Britanova, O; Bormuth, I; Donahoo, AL; Ackerman, SL; Richards, LJ; Tarabykin, V
      Nature communications 5 3708 2014

      Show Abstract
      24739528 24739528
      Astrocytes refine cortical connectivity at dendritic spines.
      Risher, WC; Patel, S; Kim, IH; Uezu, A; Bhagat, S; Wilton, DK; Pilaz, LJ; Singh Alvarado, J; Calhan, OY; Silver, DL; Stevens, B; Calakos, N; Soderling, SH; Eroglu, C
      eLife 3 2014

      Show Abstract
      25517933 25517933
      Neuron glia-related cell adhesion molecule (NrCAM) promotes topographic retinocollicular mapping.
      Dai, J; Buhusi, M; Demyanenko, GP; Brennaman, LH; Hruska, M; Dalva, MB; Maness, PF
      PloS one 8 e73000 2013

      Show Abstract
      24023801 24023801
      ADAM17 is critical for multipolar exit and radial migration of neuronal intermediate progenitor cells in mice cerebral cortex.
      Li, Q; Zhang, Z; Li, Z; Zhou, M; Liu, B; Pan, L; Ma, Z; Zheng, Y
      PloS one 8 e65703 2013

      Show Abstract
      23755270 23755270
      Neuronal cadherin (NCAD) increases sensory neurite formation and outgrowth on astrocytes.
      Ferguson, TA; Scherer, SS
      Neuroscience letters 522 108-12 2012

      Show Abstract
      22698587 22698587
      EphB regulates L1 phosphorylation during retinocollicular mapping.
      Jinxia Dai,Jasbir S Dalal,Sonal Thakar,Mark Henkemeyer,Vance P Lemmon,Jill S Harunaga,Monika C Schlatter,Mona Buhusi,Patricia F Maness
      Molecular and cellular neurosciences 50 2012

      Show Abstract
      22579729 22579729
      Evidence that descending cortical axons are essential for thalamocortical axons to cross the pallial-subpallial boundary in the embryonic forebrain.
      Chen, Y; Magnani, D; Theil, T; Pratt, T; Price, DJ
      PloS one 7 e33105 2012

      Show Abstract
      22412988 22412988
      Evidence for a role of srGAP3 in the positioning of commissural axons within the ventrolateral funiculus of the mouse spinal cord.
      Bacon, C; Endris, V; Andermatt, I; Niederkofler, V; Waltereit, R; Bartsch, D; Stoeckli, ET; Rappold, G
      PloS one 6 e19887 2011

      Show Abstract Full Text Article
      21655271 21655271
      Heparan sulfate sugar modifications mediate the functions of slits and other factors needed for mouse forebrain commissure development.
      Conway, CD; Howe, KM; Nettleton, NK; Price, DJ; Mason, JO; Pratt, T
      The Journal of neuroscience : the official journal of the Society for Neuroscience 31 1955-70 2011

      Show Abstract
      21307234 21307234

      Data Sheet

      Title
      Anti-Neural Cell Adhesion Molecule L1, clone 324 - Data Sheet