MAB377X Anti-NeuN Antibody, clone A60, Alexa Fluor®488 conjugated

MAB377X
500 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R IHC M AlexaFluor®488 Monoclonal Antibody
      Description
      Catalogue NumberMAB377X
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-NeuN Antibody, clone A60, Alexa Fluor®488 conjugated
      Alternate Names
      • Neuron-Specific Nuclear Protein
      Background InformationNeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found (Mullen et al., 1992).
      References
      Product Information
      FormatAlexaFluor®488
      Control
      • Brain tissue, most neuronal cell types throughout the adult nervous system
      PresentationPurified immunoglobulin conjugated to Alexa Fluor® 488. Liquid in Phosphate buffer with 15 mg/mL BSA as a stabilizer and 0.1% sodium azide.
      Applications
      ApplicationAnti-NeuN Antibody, clone A60, Alexa Fluor®488 conjugated is an antibody against NeuN for use in IH.
      Key Applications
      • Immunohistochemistry
      Application NotesImmunohistochemistry: 1:100 on rat (paraformaldehyde fixed) and mouse (paraformaldehyde fixed, antigen retrieved) brain tissue.



      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified cell nuclei from mouse brain.
      CloneA60
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityVertebrate neuron-specific nuclear protein called NeuN (Neuronal Nuclei). MAB377X reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia. The immunohistochemical staining is primarily in the nucleus of the neurons with lighter staining in the cytoplasm. The few cell types not reactive with MAB377X include Purkinje, mitral and photoreceptor cells.

      Developmentally, immunoreactivity is first observed shortly after neurons have become postmitotic, no staining has been observed in proliferative zones.

      The antibody is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • NeuN
      • A60
      Purification MethodProtein A Purfied
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 6 months at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size500 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-NeuN Antibody, clone A60, Alexa Fluor®488 conjugated Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-NEURONAL NUCLEI (NeuN) ALEXA FLUOR 488 CONJUGATED MONOCLONAL ANTIBODY - 23773702377370
      MOUSE ANTI-NEURONAL NUCLEI (NeuN) ALEXA FLUOR 488 CONJUGATED MONOCLONAL ANTIBODY - 24286872428687
      MOUSE ANTI-NEURONAL NUCLEI (NeuN) ALEXA FLUOR 488 CONJUGATED MONOCLONAL ANTIBODY - 24468662446866
      MOUSE ANTI-NEURONAL NUCLEI (NeuN) - 32679343267934
      MOUSE ANTI-NEURONAL NUCLEI (NeuN) - 33082243308224
      MOUSE ANTI-NEURONAL NUCLEI (NeuN) -25103272510327
      MOUSE ANTI-NEURONAL NUCLEI (NeuN) -25237932523793
      MOUSE ANTI-NEURONAL NUCLEI (NeuN) -25673102567310
      MOUSE ANTI-NEURONAL NUCLEI (NeuN) -26069022606902
      MOUSE ANTI-NEURONAL NUCLEI (NeuN) -26530912653091

      References

      Reference overviewApplicationSpeciesPub Med ID
      Involvement of phosphatase and tensin homolog deleted from chromosome 10 in rodent model of neuropathic pain.
      Huang, SY; Sung, CS; Chen, WF; Chen, CH; Feng, CW; Yang, SN; Hung, HC; Chen, NF; Lin, PR; Chen, SC; Wang, HM; Chu, TH; Tai, MH; Wen, ZH
      Journal of neuroinflammation  12  59  2015

      Show Abstract
      25889774 25889774
      CXCL12 in astrocytes contributes to bone cancer pain through CXCR4-mediated neuronal sensitization and glial activation in rat spinal cord.
      Shen, W; Hu, XM; Liu, YN; Han, Y; Chen, LP; Wang, CC; Song, C
      Journal of neuroinflammation  11  75  2014

      Show Abstract
      24735601 24735601
      Non-Gaussian diffusion imaging for enhanced contrast of brain tissue affected by ischemic stroke.
      Grinberg, F; Farrher, E; Ciobanu, L; Geffroy, F; Le Bihan, D; Shah, NJ
      PloS one  9  e89225  2014

      Show Abstract
      24586610 24586610
      Ifit2 deficiency results in uncontrolled neurotropic coronavirus replication and enhanced encephalitis via impaired alpha/beta interferon induction in macrophages.
      Butchi, NB; Hinton, DR; Stohlman, SA; Kapil, P; Fensterl, V; Sen, GC; Bergmann, CC
      Journal of virology  88  1051-64  2014

      Show Abstract
      24198415 24198415
      Synthesis, transport, and metabolism of serotonin formed from exogenously applied 5-HTP after spinal cord injury in rats.
      Li, Y; Li, L; Stephens, MJ; Zenner, D; Murray, KC; Winship, IR; Vavrek, R; Baker, GB; Fouad, K; Bennett, DJ
      Journal of neurophysiology  111  145-63  2014

      Show Abstract
      Immunohistochemistry24068759 24068759
      HMG-CoA Reductase Inhibition Promotes Neurological Recovery, Peri-Lesional Tissue Remodeling, and Contralesional Pyramidal Tract Plasticity after Focal Cerebral Ischemia.
      Kilic, E; Reitmeir, R; Kilic, Ü; Caglayan, AB; Beker, MC; Kelestemur, T; Ethemoglu, MS; Ozturk, G; Hermann, DM
      Frontiers in cellular neuroscience  8  422  2014

      Show Abstract
      25565957 25565957
      Prevention of JNK phosphorylation as a mechanism for rosiglitazone in neuroprotection after transient cerebral ischemia: activation of dual specificity phosphatase.
      Okami, N; Narasimhan, P; Yoshioka, H; Sakata, H; Kim, GS; Jung, JE; Maier, CM; Chan, PH
      Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism  33  106-14  2013

      Show Abstract
      Western Blotting23032483 23032483
      Alterations in sulfated chondroitin glycosaminoglycans following controlled cortical impact injury in mice.
      Yi, JH; Katagiri, Y; Susarla, B; Figge, D; Symes, AJ; Geller, HM
      The Journal of comparative neurology  520  3295-313  2012

      Show Abstract
      ImmunofluorescenceMouse22628090 22628090
      Characterization of inducible models of Tay-Sachs and related disease.
      Sargeant, TJ; Drage, DJ; Wang, S; Apostolakis, AA; Cox, TM; Cachón-González, MB
      PLoS genetics  8  e1002943  2012

      Show Abstract
      23028353 23028353
      Impairment of retrograde neuronal transport in oxaliplatin-induced neuropathy demonstrated by molecular imaging.
      Schellingerhout, D; LeRoux, LG; Hobbs, BP; Bredow, S
      PloS one  7  e45776  2012

      Show Abstract
      23029238 23029238

      Data Sheet

      Title
      Anti-NeuN, clone A60, AlexaFluor 488 conjugated - Data Sheet

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      Alternative Format

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      Life Science Research > Antibodies and Assays > Primary Antibodies