MAB377 Anti-NeuN Antibody, clone A60

MAB377
500 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Av, Ch, Ft, H, M, Po, R, Sal FC, ICC, IF, IHC, IH(P), IP, WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB377
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-NeuN Antibody, clone A60
      Alternate Names
      • Neuron-Specific Nuclear Protein
      • Neuna60
      • A60
      Background InformationNeuN antibody (NEUronal Nuclei; clone A60) specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples (Mullen et al., 1992; Wolf et al., 1996). Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron (Mullen et al., 1992). Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found (Mullen et al., 1992).
      References
      Product Information
      FormatPurified
      Control
      • Positive control -Brain Tissue. Negative control - Any non neuronal tissue eg Fibroblasts
      PresentationPurified mouse immunoglobulin IgG1 liquid in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
      Applications
      ApplicationAnti-NeuN Antibody, clone A60 detects level of NeuN and has been published and validated for use in FC, IC, IF, IH, IH(P), IP and WB.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blot Analysis:
      A previous lot of this antibody recognized 2-3 bands in the 46-48 kDa range and possibly another band at approximately 66 kDa.

      Immunocytochemistry:
      1:10-1:100 dilution from a previous lot was used. Neurons in culture should be permeablized with 0.1% triton X-100. All primary antibody dilutions should be performed with simple solutions containing only buffer and primary antibody without excess protein blocks or detergents.

      Immunohistochemistry:
      1:100-1:1,000. The antibody works best on polyester wax embedded tissue but also works on paraffin embedded tissue at a lower working dilution. The antibody works well with formaldehyde-based fixatives. Citric acid and microwave pretreatment has been used successfully (Sarnat, 1998).

      Immunohistochemistry(paraffin) Analysis: A previous lot was used for IH(P).

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified cell nuclei from mouse brain
      CloneA60
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityMILLIPORE's exclusive monoclonal antibody to vertebrate neuron-specific nuclear protein called NeuN (or Neuronal Nuclei) reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia. Developmentally, immunoreactivity is first observed shortly after neurons have become postmitotic, no staining has been observed in proliferative zones. The immunohistochemical staining is primarily localized in the nucleus of the neurons with lighter staining in the cytoplasm. The few cell types not reactive with MAB377 include Purkinje, mitral and photoreceptor cells. The antibody is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants.
      IsotypeIgG1
      Species Reactivity
      • Avian
      • Chicken
      • Ferret
      • Human
      • Mouse
      • Pig
      • Rat
      • Salamander
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • NeuN
      • A58
      Purification MethodProtein A Purfied
      Molecular Weight46/48 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunohistochemistry on brain tissue.

      Immunohistochemistry(paraffin) Analysis:
      NeuN (cat. # MAB377) staining pattern/morphology in rat cerebellum. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seen as nuclear staining in the neurons in the granular layer. Note that there is no signal detected in the nucleus of Purkinje cells.
      Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8ºC from date of receipt.
      Packaging Information
      Material Size500 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-NeuN Antibody, clone A60 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-NeuN Antibody, clone A60 Certificates of Analysis

      TitleLot Number
      Anti-NeuN, clone A603075598
      Anti-NeuN, clone A60 - 21180332118033
      Anti-NeuN, clone A60 - 23756082375608
      Anti-NeuN, clone A60 - 23922832392283
      Anti-NeuN, clone A60 - 24245072424507
      Anti-NeuN, clone A60 - 24286712428671
      Anti-NeuN, clone A60 - 24532492453249
      Anti-NeuN, clone A60 - 19912631991263
      Anti-NeuN, clone A60 - 20623132062313
      Anti-NeuN, clone A60 - 20747652074765

      References

      Reference overviewApplicationSpeciesPub Med ID
      Pre-existing astrocytes form functional perisynaptic processes on neurons generated in the adult hippocampus.
      Krzisch, M; Temprana, SG; Mongiat, LA; Armida, J; Schmutz, V; Virtanen, MA; Kocher-Braissant, J; Kraftsik, R; Vutskits, L; Conzelmann, KK; Bergami, M; Gage, FH; Schinder, AF; Toni, N
      Brain structure & function  220  2027-42  2015

      Show Abstract
      24748560 24748560
      Enrichment rescues contextual discrimination deficit associated with immediate shock.
      Clemenson, GD; Lee, SW; Deng, W; Barrera, VR; Iwamoto, KS; Fanselow, MS; Gage, FH
      Hippocampus  25  385-92  2015

      Show Abstract
      25330953 25330953
      An anterograde rabies virus vector for high-resolution large-scale reconstruction of 3D neuron morphology.
      Haberl, MG; Viana da Silva, S; Guest, JM; Ginger, M; Ghanem, A; Mulle, C; Oberlaender, M; Conzelmann, KK; Frick, A
      Brain structure & function  220  1369-79  2015

      Show Abstract
      24723034 24723034
      Nicotine accelerates diabetes-induced retinal changes.
      Boretsky, A; Gupta, P; Tirgan, N; Liu, R; Godley, BF; Zhang, W; Tilton, RG; Motamedi, M
      Current eye research  40  368-77  2015

      Show Abstract
      24911405 24911405
      PERK inhibition prevents tau-mediated neurodegeneration in a mouse model of frontotemporal dementia.
      Radford, H; Moreno, JA; Verity, N; Halliday, M; Mallucci, GR
      Acta neuropathologica  130  633-42  2015

      Show Abstract
      26450683 26450683
      Corelease of acetylcholine and GABA from cholinergic forebrain neurons.
      Saunders, A; Granger, AJ; Sabatini, BL
      eLife  4  2015

      Show Abstract
      25723967 25723967
      The serine protease inhibitor SerpinA3N attenuates neuropathic pain by inhibiting T cell-derived leukocyte elastase.
      Vicuña, L; Strochlic, DE; Latremoliere, A; Bali, KK; Simonetti, M; Husainie, D; Prokosch, S; Riva, P; Griffin, RS; Njoo, C; Gehrig, S; Mall, MA; Arnold, B; Devor, M; Woolf, CJ; Liberles, SD; Costigan, M; Kuner, R
      Nature medicine  21  518-23  2015

      Show Abstract
      25915831 25915831
      Quantitative and functional interrogation of parent-of-origin allelic expression biases in the brain.
      Perez, JD; Rubinstein, ND; Fernandez, DE; Santoro, SW; Needleman, LA; Ho-Shing, O; Choi, JJ; Zirlinger, M; Chen, SK; Liu, JS; Dulac, C
      eLife  4  e07860  2015

      Show Abstract
      26140685 26140685
      Milnacipran remediates impulsive deficits in rats with lesions of the ventromedial prefrontal cortex.
      Tsutsui-Kimura, I; Yoshida, T; Ohmura, Y; Izumi, T; Yoshioka, M
      The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum (CINP)  18  2015

      Show Abstract
      25522418 25522418
      Modeling the interaction between quinolinate and the receptor for advanced glycation end products (RAGE): relevance for early neuropathological processes.
      Serratos, IN; Castellanos, P; Pastor, N; Millán-Pacheco, C; Rembao, D; Pérez-Montfort, R; Cabrera, N; Reyes-Espinosa, F; Díaz-Garrido, P; López-Macay, A; Martínez-Flores, K; López-Reyes, A; Sánchez-García, A; Cuevas, E; Santamaria, A
      PloS one  10  e0120221  2015

      Show Abstract
      25757085 25757085

      Brochure

      Title
      SNAP i.d. 2.0 System Brochure

      Data Sheet

      Title
      Anti-NeuN, clone A60 - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies