A part of MilliporeSigma

ABN91 | Anti-NeuN Antibody

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M IH(P), WB, ICC Ch Affinity Purified Polyclonal Antibody
      Catalogue NumberABN91
      DescriptionAnti-NeuN Antibody
      Alternate Names
      • RNA binding protein fox-1 homolog 3
      • Fox-1 homolog C
      Background InformationNeuN (RNA binding protein fox-1 homolog 3; Fox-1 homolog C) is a RNA-binding protein found exclusively in the nuclei of neuronal cells. It is a member of the evolutionarily conserved Fox-1 family and is mainly involved in splicing of RNA.
      Product Information
      FormatAffinity Purified
      • Mouse brain E16 tissue lysate
      PresentationPurified chicken polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationThis Anti-NeuN Antibody is validated for use in IHC(P), Western Blotting, ICC for the detection of NeuN.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NeuN in rat E18 cortex cells.

      Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected NeuN in mouse brain, mouse hippocampus, and mouse frontal cortex tissues.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the N-terminus of mouse NeuN.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes the N-terminus of NeuN.
      Species Reactivity
      • Rat
      • Mouse
      Species Reactivity NoteDemonstrated to react with Mouse and Rat. Other homologies: Human (94% sequence homology).
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • NeuN
      Purification MethodAffinity Purfied
      Molecular Weight~45 kDa observed. Uncharacterized bands appear at ~40 and ~50 kDa in some lysates.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse brain E16 tissue lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected NeuN on 10 µg of mouse brain E16 tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information