A part of MilliporeSigma

MABN101 | Anti-Netrin receptor DCC Antibody, clone 2A11.1

200 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R IHC, WB M Purified Monoclonal Antibody
      Catalogue NumberMABN101
      DescriptionAnti-Netrin receptor DCC Antibody, clone 2A11.1
      Alternate Names
      • Colorectal cancer suppressor
      • Immunoglobulin superfamily DCC subclass member 1
      • Tumor suppressor protein DCC
      Background InformationDeleted in colorectal cancer (DCC) protein is a netrin receptor that plays an important role in axon guidance in the developing nervous system. The DCC receptor is expressed specifically on filopodia and lamellipodia of neural growth cones, and promotes directional movement and growth of these areas, where secreted netrin-1 is detected. The DCC receptor may produce these effects through induction of intracellular signaling cascades and interaction with a diverse group of proteins. Previous studies have suggested that ligand-bound DCC signals lead to remodeling of the actin cytoskeleton, a process which may involve Cdc42, Rac1, and MAPK signaling. DCC receptor may also mediate turning movements of growth cones in association with the Down's syndrome cell adhesion molecule (DSCAM) receptor.
      Product Information
      • E13-E14 brain tissue lysate
      PresentationPurified mouse monoclonal IgG2aκ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-Netrin receptor DCC Antibody, clone 2A11.1 is an antibody against Netrin receptor DCC for use in IHC, Western Blotting.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Netrin receptor DCC in rat cerebellum and rat hippocampus tissues.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to mouse Netrin receptor DCC.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a netrin 1 receptor. The transmembrane protein is a member of the immunoglobulin superfamily of cell adhesion molecules, and mediates axon guidance of neuronal growth cones towards sources of netrin 1 ligand. The cytoplasmic tail interacts with the tyrosine kinases Src and focal adhesion kinase (FAK, also known as PTK2) to mediate axon attraction. The protein partially localizes to lipid rafts, and induces apoptosis in the absence of ligand. The protein functions as a tumor suppressor, and is frequently mutated or downregulated in colorectal cancer and esophageal carcinoma.
      Gene Symbol
      • DCC
      • IGDCC1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Receptor for netrin required for axon guidance. Mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Implicated as a tumor suppressor gene.

      SUBUNIT STRUCTURE: Interacts with the cytoplasmic part of UNC5A, UNC5B, UNC5C and probably UNC5D. Interacts with DSCAM. Interacts with PTK2/FAK1.

      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

      TISSUE SPECIFICITY: Found in axons of the central and peripheral nervous system and in differentiated cell types of the intestine. Not expressed in colorectal tumor cells that lost their capacity to differentiate into mucus producing cells.

      POST-TRANSLATIONAL MODIFICATION: Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.

      INVOLVEMENT IN DISEASE: Defects in DCC are the cause of congenital mirror movements (MIMOC). Mirror movements are contralateral involuntary movements that mirror voluntary ones. While mirror movements are occasionally found in young children, persistence beyond the age of 10 is abnormal. Mirror movements occur more commonly in the upper extremities.

      MISCELLANEOUS: Inactivation of DCC due to allelic deletion and/or point mutations is related to lymphatic and hematogenous metastatic tumor dissemination.

      SEQUENCE SIMILARITIES: Belongs to the immunoglobulin superfamily. DCC family. Contains 6 fibronectin type-III domains.

      Contains 4 Ig-like C2-type (immunoglobulin-like) domains.
      Molecular Weight~160 kDa observed. Uncharacterized bands may be observed at ~24 kDa and ~60 kDa in some tissue lysates.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse E13-E14 brain tissue lysate.

      Western Blot Analysis: 2 µg/mL of this antibody detected Netrin receptor DCC in 10 µg of mouse E13-E14 brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information