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MAB353C3 | Anti-Nestin Antibody, clone rat-401, Cy3 conjugate

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R ICC M Cy3 Monoclonal Antibody
      Catalogue NumberMAB353C3
      DescriptionAnti-Nestin Antibody, clone rat-401, Cy3 conjugate
      Alternate Names
      • rat401
      • NES
      • Nbla00170
      • FLJ21841
      Background InformationNestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells (Mokry and Nemecek, 1998). Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.
      Product Information
      • Mouse cortical neural stem cells (SCR029)
      PresentationPurified mouse monoclonal IgG1 conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.
      ApplicationThis Anti-Nestin Antibody, clone rat-401, Cy3 conjugate is validated for use in ICC for the detection of Nestin.
      Key Applications
      • Immunocytochemistry
      Biological Information
      ImmunogenEmbryonic rat spinal cord
      SpecificityThis antibody recognizes both rat and mouse Nestin. Antibody is not reactive with human Nestin protein.
      Species Reactivity
      • Mouse
      • Rat
      Species Reactivity NoteDemonstrated to react with Mouse and Rat. Antibody is not reactive with human Nestin protein.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NES
      • Nbla00170
      • FLJ21841
      Purification MethodProtein G Purified
      UniProt Number
      Molecular WeightThe unconjugated parent antibody (Catalog No. MAB353) has an observed MW at 220-240 kDA.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in mouse cortical neural stem cells (SCR029). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in mouse cortical neural stem cells (SCR029).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Nestin, clone rat-401, Cy3 -2631549 2631549
      Anti-Nestin, clone rat-401, Cy3 -2654777 2654777
      Anti-Nestin, clone rat-401, Cy3 -2816566 2816566
      Anti-Nestin, clone rat-401, Cy3 conjugate - 2382221 2382221
      Anti-Nestin, clone rat-401, Cy3 conjugate - 2199688 2199688
      Anti-Nestin, clone rat-401, Cy3 conjugate - 2317807 2317807
      Anti-Nestin, clone rat-401, Cy3 conjugate - Q2025116 Q2025116
      Anti-Nestin, clone rat-401, Cy3 conjugate -2518499 2518499


      Reference overviewPub Med ID
      Differentiation of non-mesencephalic neural stem cells towards dopaminergic neurons.
      Rössler, R, et al.
      Neuroscience, 170: 417-28 (2010) 2010

      Show Abstract
      20643196 20643196
      Absence of annulus in human asthenozoospermia: case report.
      Lhuillier, P, et al.
      Hum. Reprod., 24: 1296-303 (2009) 2009

      Show Abstract
      19221096 19221096
      Developmental changes and injury induced disruption of the radial organization of the cortex in the immature rat brain revealed by in vivo diffusion tensor MRI.
      Sizonenko, SV; Camm, EJ; Garbow, JR; Maier, SE; Inder, TE; Williams, CE; Neil, JJ; Huppi, PS
      Cerebral cortex (New York, N.Y. : 1991) 17 2609-17 2007

      Show Abstract
      17259644 17259644
      Intermediate filaments in cardiac myogenesis: nestin in the developing mouse heart
      Kachinsky, A. et al.
      J Histochem Cytochem. , 43:843-847 (1995) 1995

      7542682 7542682

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      Life Science Research > Antibodies and Assays > Primary Antibodies