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ABT319 | Anti-Nephrin Antibody

100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IH(P) Rb Affinity Purified Polyclonal Antibody
      Catalogue NumberABT319
      DescriptionAnti-Nephrin Antibody
      Alternate Names
      • Nephrin
      • Renal glomerulus-specific cell adhesion receptor
      Background InformationNephrin (NPHS1, NPHN), also known as is Renal glomerulus-specific cell adhesion receptor, is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm.
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-Nephrin Antibody is a highly specific rabbit polyclonal antibody, that targets Nephrin & has been tested in western blotting & IHC (Paraffin).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Nephrin in human kidney tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the NPHS2 binding domain of human Nephrin.
      EpitopeNPHS2 binding domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody is expected to detect both known isoforms of Nephrin.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NPHS1
      • NPHN
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~190 kDa and ~85 kDa observed. Two isoforms are known to exist at 134kDa (Isoform 1) and 130kDa (Isoform 2). This protein may be observed at higher molecular weights due to glycosylation (Ruotsalainen, V., et al. (1999). Proc Natl Acad Sci USA. 6(96):7962-7967.).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human kidney tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Nephrin in 10 µg of human kidney tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies