MAB3140 Anti-Na+/H+ Exchanger-1 Antibody, CT, clone 4E9

MAB3140
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Vrt, F, Am, Av, Ma WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB3140
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Na+/H+ Exchanger-1 Antibody, CT, clone 4E9
      Alternate Names
      • NHE1
      Background InformationMammalian NHE (Na+/H+ Exchanger) is an integral membrane protein that is known to regulate intracellular pH by removing a proton in exchange for an extracellular sodium ion. There are nine known isofoms of NHE. The best characterized is NHE1, involved not only in intracellular pH control, but cell-volume control, cytoskeletal organization, heart disease and cancer. Transport by the NHE plays a pivotal role in the damage caused to the human myocardium during and following a myocardial infarction, and it is considered to represent a key step in the oncogenic transformation of cancerous cells. NHE comprises an N-terminal membrane domain that transports ions, and a C-terminal cytoplasmic domain that regulates activity and mediates cytoskeletal interactions. NHE2 - NHE5 isoforms are localized to the plasma membrane but exhibit greater tissue-specificity. NH2 and NH3 are more highly expressed in the kidney and intestine. Structurally, a single transmembrane segment from NHE1 has been solved, and the structure of bacterial Na+/H+ antiporter NhaA has been elucidated.
      References
      Product Information
      FormatPurified
      Control
      • Kidney tissue (basolateral membrane)
      PresentationPurified mouse monoclonal IgG1 liquid in buffer containing 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide
      Applications
      ApplicationAnti-Na+/H+ Exchanger-1 Antibody, C-terminus, clone 4E9 detects level of Na+/H+ Exchanger-1 & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Applications Not Recommended
      • Immunoprecipitation
      Application NotesWorks poorly for immunohistochemistry and immunocytochemistry.
      Not recommended for immunoprecipitation.

      Immunoblotting:
      1:1,000 dilution used on a previous lot.

      Optimal working dilutions must be determined by the user.
      Biological Information
      ImmunogenMBP fusion protein containing the entire C-terminal, hydrophylic domain of porcine NHE1.
      EpitopeC-terminus
      Clone4E9
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityNa+/H+ exchanger, isoform NHE1
      IsotypeIgG1
      Species Reactivity
      • Vertebrates
      • Fish
      • Amphibian
      • Avian
      • Mammals
      Species Reactivity NoteAll vertebrates including fish, amphibians, birds and mammals. Expected to react with rat and mouse based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SLC9A1
      • FLJ42224
      • APNH
      • NHE-1
      • Na
      • +H
      • NHE1
      • APNH1
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P19634 # Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
      SIZE: 815 amino acids; 90763 Da
      SUBUNIT: Interacts with tescalcin, CHP and CHP2.
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Kidney and intestine.
      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. & O-glycosylated.
      SIMILARITY: SwissProt: P19634 ## Belongs to the Na(+)/H(+) exchanger (TC 2.A.36.5.1) family.
      MISCELLANEOUS: Inhibited by amiloride and 5-amino-substituted derivatives and activated in a cooperative fashion by intracellular H(+). In quiescent cells upon growth factor stimulation, the apparent affinity for internal H(+) is increased, resulting in a persistent rise in cytoplasmic pH.
      Molecular Weight~100-110 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Human Kidney lysates.

      Western Blot:
      1:500 dilution of this lot detected Na+/H+ Exchanger-1 on 10 μg of Human Kidney lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Na+/H+ Exchanger-1, C-terminus, -28138642813864
      Anti-Na+/H+ Exchanger-1, C-terminus, clone 4E93065559
      Anti-Na+/H+ Exchanger-1, C-terminus, clone 4E9 - 19979201997920
      Anti-Na+/H+ Exchanger-1, C-terminus, clone 4E9 - 20415762041576
      Anti-Na+/H+ Exchanger-1, C-terminus, clone 4E9 - 22838522283852
      Anti-Na+/H+ Exchanger-1, C-terminus, clone 4E9 - 28930312893031
      Anti-Na+/H+ Exchanger-1, C-terminus, clone 4E9 - 31532853153285
      Anti-Na+/H+ Exchanger-1, C-terminus, clone 4E9 - LV1414355LV1414355
      Anti-Na+/H+ Exchanger-1, C-terminus, clone 4E9 - LV1580845LV1580845
      Anti-Na+/H+ Exchanger-1, C-terminus, clone 4E9 - LV1757001LV1757001

      References

      Reference overviewApplicationSpeciesPub Med ID
      Loss of the AE3 Cl(-)/HCO(-) 3 exchanger in mice affects rate-dependent inotropy and stress-related AKT signaling in heart.
      Prasad, V; Lorenz, JN; Lasko, VM; Nieman, ML; Al Moamen, NJ; Shull, GE
      Frontiers in physiology  4  399  2013

      Show Abstract
      24427143 24427143
      The 10-20-30 training concept improves performance and health profile in moderately trained runners.
      Gunnarsson, TP; Bangsbo, J
      Journal of applied physiology (Bethesda, Md. : 1985)  113  16-24  2012

      Show Abstract
      22556401 22556401
      Four weeks of normobaric "live high-train low" do not alter muscular or systemic capacity for maintaining pH and K⁺ homeostasis during intense exercise.
      Nordsborg, NB; Siebenmann, C; Jacobs, RA; Rasmussen, P; Diaz, V; Robach, P; Lundby, C
      Journal of applied physiology (Bethesda, Md. : 1985)  112  2027-36  2012

      Show Abstract
      Western BlottingHuman22461443 22461443
      Silencing of NHE-1 blunts the slow force response to myocardial stretch.
      Pérez, NG; Nolly, MB; Roldan, MC; Villa-Abrille, MC; Cingolani, E; Portiansky, EL; Alvarez, BV; Ennis, IL; Cingolani, HE
      Journal of applied physiology (Bethesda, Md. : 1985)  111  874-80  2011

      Show Abstract
      21659487 21659487
      Structural modeling and electron paramagnetic resonance spectroscopy of the human Na+/H+ exchanger isoform 1, NHE1.
      Nygaard, EB; Lagerstedt, JO; Bjerre, G; Shi, B; Budamagunta, M; Poulsen, KA; Meinild, S; Rigor, RR; Voss, JC; Cala, PM; Pedersen, SF
      The Journal of biological chemistry  286  634-48  2011

      Show Abstract Full Text Article
      20974853 20974853
      Protein Phosphatase 2A (B55{alpha}) Prevents Premature Activation of Forkhead Transcription Factor FoxM1 by Antagonizing Cyclin A/ Cyclin-dependent Kinase
      Alvarez-Fernández M, Halim VA, Aprelia M, Mohammed S, Medema RH
      The Journal of biological chemistry  286  33029-36. Epub 2011 Aug 3.  2011

      Show Abstract
      21813648 21813648
      Silencing of cardiac mitochondrial NHE1 prevents mitochondrial permeability transition pore opening.
      Villa-Abrille, MC; Cingolani, E; Cingolani, HE; Alvarez, BV
      American journal of physiology. Heart and circulatory physiology  300  H1237-51  2011

      Show Abstract
      21297023 21297023
      Exercise-induced regulation of muscular Na+-K+ pump, FXYD1, and NHE1 mRNA and protein expression: importance of training status, intensity, and muscle type.
      Rasmussen, MK; Juel, C; Nordsborg, NB
      American journal of physiology. Regulatory, integrative and comparative physiology  300  R1209-20  2011

      Show Abstract
      21325644 21325644
      Relationship between performance at different exercise intensities and skeletal muscle characteristics.
      Iaia, FM; Perez-Gomez, J; Thomassen, M; Nordsborg, NB; Hellsten, Y; Bangsbo, J
      Journal of applied physiology (Bethesda, Md. : 1985)  110  1555-63  2011

      Show Abstract
      21436467 21436467
      Silencing of sodium/hydrogen exchanger in the heart by direct injection of naked siRNA.
      Morgan, PE; Correa, MV; Ennis, IL; Ennis, IE; Diez, AA; Pérez, NG; Cingolani, HE
      Journal of applied physiology (Bethesda, Md. : 1985)  111  566-72  2011

      Show Abstract
      21596922 21596922

      Data Sheet

      Title
      Anti-Na+/H+ Exchanger-1, C-terminus, clone 4E9 - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies