AB1548 Anti-NMDAR2A&B Antibody, pan

AB1548
50 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Av, Gp, H, M, R, Rb IH(P), ICC, IHC, IP, WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB1548
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-NMDAR2A&B Antibody, pan
      References
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified using the immunogen peptide. Lyophilized from PBS containing 1% BSA.



      Note: lot 0509010940 was shipped in liquid format; Do NOT add additional water to this lot of material.
      Applications
      ApplicationAnti-NMDAR2A&B Antibody, pan detects level of NMDAR2A&B & has been published & validated for use in IH(P), IC, IH, IP & WB.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern blot analysis can be done at final concentrations of 0.25-0.5 μg/mL; recognizes a single band in membrane preparations of rat hippocampal neurons ~170,000-180,000 under reduced, SDS PAGE. AB1548 can be used for immunocytochemistry using paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Final concentrations recommended are 0.5-1.0 μg/mL.

      The antibody can be used for immunopreciptation of detergent solubilized receptor from brain or transfected cells. For most cases 5 μg of antibody is used with immobilized Protein A.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide (LNSCSNRRVYKKMPSIESDV) corresponding to the C-terminus of rat NMDAR2A receptor subunit conjugated to BSA.
      HostRabbit
      SpecificityWestern blot analysis of transfected cells shows the antibody recognizes both NR2A and NR2B subunits equally. There may be a very slight recognition of NR2C and NR2D. Western blot analysis of rat brain shows a single band comigrating with NMDAR2A and NMDAR2B expressed in transfected cells. AB1548 has also been used effectively in Western blot of the membrane fraction of rabbit cortex and avian brain homogenates. No cross-reaction is seen with NMDAR1 or other glutamate receptor subunits.
      Species Reactivity
      • Avian
      • Guinea Pig
      • Human
      • Mouse
      • Rat
      • Rabbit
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryN-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain.
      Gene Symbol
      • GRIN2A
      • hNR2A
      • Glutamate
      • NMDAR2A
      • NR2A
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13224 # NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
      SIZE: 1484 amino acids; 166367 Da
      SUBUNIT: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1 and GRIN3B. Found in a complex with GRIN1, GRIN3A and PPP2CB. Interacts with PDZ domains of INADL and DLG4 (By similarity).
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
      SIMILARITY: SwissProt: Q13224 ## Belongs to the glutamate-gated ion channel (TC 1.A.10) family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at 2-8°C or -20°C. Reconstitute to 500 μL with sterile distilled water. Store reconstituted material frozen (-20°C or -80°C) in undiluted aliquots for up to six months. Avoid repeated thawing and refreezing. Diluted antibody containing 0.1% sodium azide for Western blot analysis can be stored for several weeks and reused repeatedly.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-NMDA RECEPTOR 2A/B2470836
      RABBIT ANTI-NMDA RECEPTOR 2A/B AFFINITY PURIFIED POLYCLONAL ANTIBODY2987108
      RABBIT ANTI-NMDA RECEPTOR 2A/B AFFINITY PURIFIED POLYCLONAL ANTIBODY2776755
      RABBIT ANTI-NMDA RECEPTOR 2A/B AFFINITY PURIFIED POLYCLONAL ANTIBODY - 24204332420433
      RABBIT ANTI-NMDA RECEPTOR 2A/B AFFINITY PURIFIED POLYCLONAL ANTIBODY - 19874641987464
      RABBIT ANTI-NMDA RECEPTOR 2A/B AFFINITY PURIFIED POLYCLONAL ANTIBODY - 20647132064713
      RABBIT ANTI-NMDA RECEPTOR 2A/B AFFINITY PURIFIED POLYCLONAL ANTIBODY - 22341392234139
      RABBIT ANTI-NMDA RECEPTOR 2A/B AFFINITY PURIFIED POLYCLONAL ANTIBODY -25064982506498

      References

      Reference overviewApplicationSpeciesPub Med ID
      High prevalence of NMDA receptor IgA/IgM antibodies in different dementia types.
      Doss, S; Wandinger, KP; Hyman, BT; Panzer, JA; Synofzik, M; Dickerson, B; Mollenhauer, B; Scherzer, CR; Ivinson, AJ; Finke, C; Schöls, L; Müller Vom Hagen, J; Trenkwalder, C; Jahn, H; Höltje, M; Biswal, BB; Harms, L; Ruprecht, K; Buchert, R; Höglinger, GU; Oertel, WH; Unger, MM; Körtvélyessy, P; Bittner, D; Priller, J; Spruth, EJ; Paul, F; Meisel, A; Lynch, DR; Dirnagl, U; Endres, M; Teegen, B; Probst, C; Komorowski, L; Stöcker, W; Dalmau, J; Prüss, H
      Annals of clinical and translational neurology  1  822-32  2014

      Show Abstract
      25493273 25493273
      Developmentally dynamic colocalization patterns of DSCAM with adhesion and synaptic proteins in the mouse retina.
      de Andrade, GB; Kunzelman, L; Merrill, MM; Fuerst, PG
      Molecular vision  20  1422-33  2014

      Show Abstract
      25352748 25352748
      Activation of EphA receptors mediates the recruitment of the adaptor protein Slap, contributing to the downregulation of N-methyl-D-aspartate receptors.
      Semerdjieva, S; Abdul-Razak, HH; Salim, SS; Yáñez-Muñoz, RJ; Chen, PE; Tarabykin, V; Alifragis, P
      Molecular and cellular biology  33  1442-55  2013

      Show Abstract
      23382070 23382070
      GluN2B in corticostriatal circuits governs choice learning and choice shifting.
      Brigman, JL; Daut, RA; Wright, T; Gunduz-Cinar, O; Graybeal, C; Davis, MI; Jiang, Z; Saksida, LM; Jinde, S; Pease, M; Bussey, TJ; Lovinger, DM; Nakazawa, K; Holmes, A
      Nature neuroscience  16  1101-10  2013

      Show Abstract
      Western Blotting23831965 23831965
      Anti-NMDA receptor encephalitis antibody binding is dependent on amino acid identity of a small region within the GluN1 amino terminal domain.
      Gleichman, AJ; Spruce, LA; Dalmau, J; Seeholzer, SH; Lynch, DR
      The Journal of neuroscience : the official journal of the Society for Neuroscience  32  11082-94  2012

      Show Abstract
      Immunocytochemistry22875940 22875940
      Hippocampal adult neurogenesis is maintained by neil3-dependent repair of oxidative DNA lesions in neural progenitor cells.
      Christine Elisabeth Regnell,Gunn Annette Hildrestrand,Yngve Sejersted,Tirill Medin,Olve Moldestad,Veslem Rolseth,Silje Zandstra Krokeide,Rajikala Suganthan,Luisa Luna,Magnar Bj,Linda H Bergersen
      Cell reports  2  2012

      Show Abstract
      22959434 22959434
      Neuroprotective effects of quercetin, rutin and okra (Abelmoschus esculentus Linn.) in dexamethasone-treated Mice.
      Tongjaroenbuangam W, Ruksee N, Chantiratikul P, Pakdeenarong N, Kongbuntad W, Govitrapong P
      Neurochemistry international  2011

      Show Abstract
      21740943 21740943
      Glutamate co-transmission from developing medial nucleus of the trapezoid body--lateral superior olive synapses is cochlear dependent in kanamycin-treated rats.
      Lee JH, Pradhan J, Maskey D, Park KS, Hong SH, Suh MW, Kim MJ, Ahn SC
      Biochem Biophys Res Commun  405  162-7. Epub 2011 Jan 5.  2011

      Show Abstract
      21215254 21215254
      Aluminum alters NMDA receptor 1A and 2A/B expression on neonatal hippocampal neurons in rats.
      Yuan, CY; Hsu, GS; Lee, YJ
      Journal of biomedical science  18  81  2011

      Show Abstract
      Immunocytochemistry22067101 22067101
      Visual experience-independent functional expression of NMDA receptors in the developing rabbit retina.
      Chang, YC; Chen, CY; Chiao, CC
      Investigative ophthalmology & visual science  51  2744-54  2010

      Show Abstract
      Immunohistochemistry20042649 20042649

      Brochure

      Title
      Pathways and Biomarkers of Glutamatergic Synapse Flyer (EMD)

      Data Sheet

      Title
      Anti-NMDAR2A&B, pain - Data Sheet
      Anti-NMDAR2A&B, pan - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies