AB1555P Anti-NMDAR2A Antibody

AB1555P
10 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, F IHC, IP, WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB1555P
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-NMDAR2A Antibody
      Alternate Names
      • N-methyl D-aspartate receptor subtype 2A
      • N-methyl-D-aspartate receptor subunit 2A
      • NMDA receptor subtype 2A
      • glutamate receptor, ionotropic
      • N-methyl D-aspartate 2A
      Background InformationNMDA (N-methyl-D-aspartate) receptors are a class of ionotropic glutamate receptors. The NMDAR channel has been shown to be involved in long term potentiation, an activity dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D).
      References
      Product Information
      FormatAffinity Purified
      Control
      • NMDAR2A is present in high concentrations in the hippocampus.
      PresentationPurified rabbit polyclonal lyophilized from ammonium bicarbonate (5 mM) so some residual salt may also be present. Reconstitute with 50 μL of PBS. Contains no preservative.
      Applications
      ApplicationAnti-NMDAR2A Antibody is an antibody against NMDAR2A for use in IH, IP & WB.
      Key Applications
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      3 μg of a previous lot was used to (under appropriate conditions) quantitatively immunoprecipitate all NMDAR2A in 200 μg of rat brain.

      Immunohistochemistry:
      A previous lot of this antibody was used at 1:1,000-1:2,000 dilution.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenC-Terminal Fusion Protein of rat NMDAR2A, aa 1253-1391.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityNMDAR2A. By Western blot it recognizes a 180 kDa band in rat brain membranes. Does not react with NMDAR2B or NMDAR2C. Immunolabeling is blocked by preadsorption of antibody with the immunogen.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Fish
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryN-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate-gated ion channels. These receptors have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C) and NMDAR2D (GRIN2D). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
      Gene Symbol
      • GRIN2A
      • hNR2A
      • Glutamate
      • NMDAR2A
      • NR2A
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFunction: NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.

      Subunit structure: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1 and GRIN3B. Found in a complex with GRIN1, GRIN3A and PPP2CB. Interacts with PDZ domains of AIP1, INADL and DLG4 By similarity.

      Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.

      Sequence similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10) family. [View classification]
      Molecular Weight180 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Rat Brain Microsomal lysates.

      Western Blot Analysis: 1:500 dilution of this lot detected NMDAR2A on 10 μg of Rat Brain Microsomal lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsLyophilized material stable for 1 year at -20°C from date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up 6 months.

      Handling Recommendations:
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-NMDAR2A2483156
      Anti-NMDAR2A - 24303412430341
      Anti-NMDAR2A - 19608481960848
      Anti-NMDAR2A - 20123542012354
      Anti-NMDAR2A - 20350622035062
      Anti-NMDAR2A - 20672202067220
      Anti-NMDAR2A - 22087682208768
      Anti-NMDAR2A - 23262922326292
      Anti-NMDAR2A - 28879982887998
      Anti-NMDAR2A - 31695263169526

      References

      Reference overviewApplicationSpeciesPub Med ID
      Developmental attenuation of N-methyl-D-aspartate receptor subunit expression by microRNAs.
      Corbel, C; Hernandez, I; Wu, B; Kosik, KS
      Neural development  10  20  2015

      Show Abstract
      26381867 26381867
      Neurological effects of inorganic arsenic exposure: altered cysteine/glutamate transport, NMDA expression and spatial memory impairment.
      Ramos-Chávez, LA; Rendón-López, CR; Zepeda, A; Silva-Adaya, D; Del Razo, LM; Gonsebatt, ME
      Frontiers in cellular neuroscience  9  21  2015

      Show Abstract
      25709567 25709567
      Repeated neonatal propofol administration induces sex-dependent long-term impairments on spatial and recognition memory in rats.
      Gonzales, EL; Yang, SM; Choi, CS; Mabunga, DF; Kim, HJ; Cheong, JH; Ryu, JH; Koo, BN; Shin, CY
      Biomolecules & therapeutics  23  251-60  2015

      Show Abstract
      25995824 25995824
      Co-Application of Corticosterone and Growth Hormone Upregulates NR2B Protein and Increases the NR2B:NR2A Ratio and Synaptic Transmission in the Hippocampus.
      Mahmoud, GS; Amer, AS
      Sultan Qaboos University medical journal  14  e486-94  2014

      Show Abstract
      25364551 25364551
      Synaptic and cognitive improvements by inhibition of 2-AG metabolism are through upregulation of microRNA-188-3p in a mouse model of Alzheimer's disease.
      Zhang, J; Hu, M; Teng, Z; Tang, YP; Chen, C
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  14919-33  2014

      Show Abstract
      25378159 25378159
      In vitro ischemia triggers a transcriptional response to down-regulate synaptic proteins in hippocampal neurons.
      Fernandes, J; Vieira, M; Carreto, L; Santos, MA; Duarte, CB; Carvalho, AL; Santos, AE
      PloS one  9  e99958  2014

      Show Abstract
      Western Blotting24960035 24960035
      Effect of D-cycloserine in conjunction with fear extinction training on extracellular signal-regulated kinase activation in the medial prefrontal cortex and amygdala in rat.
      Gupta, SC; Hillman, BG; Prakash, A; Ugale, RR; Stairs, DJ; Dravid, SM
      The European journal of neuroscience  37  1811-22  2013

      Show Abstract
      Western Blotting23551217 23551217
      Two cell circuits of oriented adult hippocampal neurons on self-assembled monolayers for use in the study of neuronal communication in a defined system.
      Edwards, D; Stancescu, M; Molnar, P; Hickman, JJ
      ACS chemical neuroscience  4  1174-82  2013

      Show Abstract
      23611164 23611164
      Asynchronous movements prior to pore opening in NMDA receptors.
      Kazi, R; Gan, Q; Talukder, I; Markowitz, M; Salussolia, CL; Wollmuth, LP
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  12052-66  2013

      Show Abstract
      23864691 23864691
      A potential neuroprotective role of apolipoprotein E-containing lipoproteins through low density lipoprotein receptor-related protein 1 in normal tension glaucoma.
      Hayashi, H; Eguchi, Y; Fukuchi-Nakaishi, Y; Takeya, M; Nakagata, N; Tanaka, K; Vance, JE; Tanihara, H
      The Journal of biological chemistry  287  25395-406  2012

      Show Abstract
      Western Blotting22674573 22674573

      Brochure

      Title
      Pathways and Biomarkers of Glutamatergic Synapse Flyer (EMD)

      Data Sheet

      Title
      Anti-NMDAR2A - Data Sheet

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      Alternative Format

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies