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ABF23 | Anti-NLRP3 Antibody

ABF23
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IH(P) Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberABF23
      DescriptionAnti-NLRP3 Antibody
      Alternate Names
      • NACHT, LRR and PYD domains-containing protein 3
      • Angiotensin/vasopressin receptor AII/AVP-like
      • Caterpiller protein 1.1
      • CLR1.1
      • Cold autoinflammatory syndrome 1 protein
      • Cryopyrin
      • PYRIN-containing APAF1-like protein 1
      • NLRP3
      Background InformationNLRPs (Nucleotide-binding oligomerization domain, Leucine rich Repeat and Pyrin domain containing Proteins) are members of NLR (Nod-like receptors) protein family. NRLP genes are known to play important roles in both the mammalian reproductive system and the innate immune system. NLRP3 may function as an inducer of apoptosis, activator of NF-kappa-B signaling and activates caspase-1 in response to triggers such as bacterial or viral infection. NLRP3 defects are linked to familial cold autoinflammatory syndrome type 1 (FCAS1), Muckle-Wells syndrome (MWS) and chronic infantile neurologic cutaneous and articular syndrome (CINCA). NLRP3 is highly expressed in blood leukocytes, polymorphonuclear cells, osteoblasts and chondrocytes.
      References
      Product Information
      FormatAffinity Purified
      Control
      • THP-1 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationDetect NLRP3 using this rabbit polyclonal antibody, Anti-NLRP3 Antibody validated for use in western blotting & IHC (Paraffin).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:4,000 dilution from a representative lot detected NLRP3 in human leukemia bone marrow and human tonsil tissue lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the DAPIN domain of human NLRP3.
      EpitopeDAPIN domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes the DAPIN domain of NLRP3.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human. Other homologies: Porcine (80% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NLRP3
      • C1orf7
      • CIAS1
      • NALP3
      • PYPAF1
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~112 kDa and ~75 kDa observed. Five isoforms are known to exist at 118 kDa, 112 kDa, 112 kDa, 106 kDa, and 84 kDa. This protein has been observed at ~75 kDa in some cell lysates (Kummer, J. A., et al. (2007). J Histochem Cytochem. 55(5):443-452.). Uncharacterized bands may be observed in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in THP-1 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected NLRP3 in 10 µg of THP-1 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies