AB5320 Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody

AB5320
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Mk ELISA, ICC, IHC, IP, WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB5320
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-NG2 Chondroitin Sulfate Proteoglycan Antibody
      Alternate Names
      • Chondroitin sulfate proteoglycan NG2
      • Melanoma chondroitin sulfate proteoglycan 3
      • Melanoma-associated chondroitin sulfate proteoglycan
      • chondroitin sulfate proteoglycan 4
      • chondroitin sulfate proteoglycan 4 (melanoma-associated)
      Background InformationChondroitin sulfate proteoglycan 4 (UniProt: Q00657; also known as Chondroitin sulfate proteoglycan NG2, HSN tumor-specific antigen) is encoded by the Cspg4 (also known as Ng2) gene (Gene ID: 81651) in rat. Chondroitin sulfate proteoglycan NG2 is a single-pass type I membrane protein that plays a role in cell proliferation and migration and stimulates endothelial cells motility during microvascular morphogenesis. It is present in neural cells and in extraneural tissues, especially in the developing mesenchyme. Its level of expression is highest on immature, proliferating cells and decreases when these cells begin to differentiate. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells. It is a major chondroitin sulfate proteoglycan that is produced after spinal cord injury and is expressed by macrophages and oligodendrocyte progenitors. It is also reported to inhibit neurite outgrowth and growth cone collapse during axon regeneration. NG2 chondroitin sulfate proteoglycan is synthesized with a signal peptide (aa 1-29), which is subsequently cleaved off in the mature form.(Ref.: Jones, LL et al. (2002). J. Neurosci. 22(7):2792-2803).
      References
      Product Information
      FormatAffinity Purified
      Control
      • Human melanoma, glioma and proliferating brain endothelial cells, rat B49 glial cell extracts or embryonic mouse brain tissue.
      PresentationPurified rabbit polyclonal in PBS buffer containing 0.02% azide.
      Applications
      ApplicationDetect NG2 Chondroitin Sulfate Proteoglycan using this Anti-NG2 Chondroitin Sulfate Proteoglycan Antibody validated for use in ELISA, IC, IH, IP and WB.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunocytochemistry:
      A 1:150-1:600 dilution of a previous lot was used in IC.

      Immunohistochemistry:
      1:200 dilution of a previous lot was used on frozen sections of embryonic mouse brain using an Alexa Fluor conjugated secondary antibody. Care should be taken to avoid overfixing tissue sections. A short (5-10 minute) fix in 2-4% PFA is recommended.

      Immunoprecipitation:
      2 μg/mL concentration of a previous lot was used in IP.

      ELISA:
      A 1:1,500-1:3,000 dilution of a previous lot was used in ELISA.

      Optimal working dilutions and protocols must be determined by end user
      Biological Information
      ImmunogenImmunoaffinity purified NG2 Chondroitin Sulfate Proteoglycan from rat.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityNG2 Chondroitin Sulfate Proteoglycan. AB5320 identifies both the intact proteoglycan and the core protein by Western blot and ELISA. When oligodendrocyte precursor cells (i.e. O-2A progenitor cells) are stained alive, the stain appears as clusters on the cell surface. This antibody does not stain differentiated oligodendrocytes well.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Monkey
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryA human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
      Gene Symbol
      • CSPG4
      • MSK16
      • MCSP
      • NG2
      • MCSPG
      • MEL-CSPG
      • HMW-MAA
      Purification MethodAntibody purified by protein A, then placed over a fibronectin affinity column.
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q6UVK1 # Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N- terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin- mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
      SIZE: 2322 amino acids; 250497 Da
      SUBUNIT: Interacts with the first PDZ domain of MPDZ. Interacts with PRKCA. Binds TNC, laminin-1, COL5A1 and COL6A2. Interacts with PLG and angiostatin. Binds FGF2 and PDGFA. Interacts with GRIP1, GRIP2 and GRIA2. Forms a ternary complex with GRIP1 and GRIA2 (By similarity). Interacts with LGALS3 and the integrin composed of ITGB1 and ITGA3. Interacts with ITGA4 through its chondroitin sulfate glycosaminoglycan. Interacts with BCAR1, CDC42 and ACK1. Interacts with MMP16.
      SUBCELLULAR LOCATION: Apical cell membrane; Single-pass type I membrane protein (By similarity). Cell projection, lamellipodium (By similarity). Note=Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes (By similarity).
      TISSUE SPECIFICITY: Detected only in malignant melanoma cells.
      PTM: N-glycosylated (By similarity). & O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4. & Phosphorylation by PRKCA regulates its subcellular location and function in cell motility (By similarity).
      SIMILARITY: SwissProt: Q6UVK1 ## Contains 15 CSPG (NG2) repeats. & Contains 2 laminin G-like domains.
      MISCELLANEOUS: Valuable marker for several incompletely differentiated precursor cells
      Molecular Weight270-300 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on rat brain lysates

      Western Blot: 1:1000 dilution of this lot detected NG2 chondroitin sulfate on 10 μg of rat brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at -20ºC in undiluted aliquots from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-NG2 Chondroitin Sulfate -26644152664415
      Anti-NG2 Chondroitin Sulfate -27684602768460
      Anti-NG2 Chondroitin Sulfate Proteoglycan3166062
      Anti-NG2 Chondroitin Sulfate Proteoglycan3162426
      Anti-NG2 Chondroitin Sulfate Proteoglycan2474971
      Anti-NG2 Chondroitin Sulfate Proteoglycan3086653
      Anti-NG2 Chondroitin Sulfate Proteoglycan - 21159922115992
      Anti-NG2 Chondroitin Sulfate Proteoglycan - 21482542148254
      Anti-NG2 Chondroitin Sulfate Proteoglycan - 23283352328335
      Anti-NG2 Chondroitin Sulfate Proteoglycan - 23888482388848

      References

      Reference overviewApplicationSpeciesPub Med ID
      Traumatic brain injury results in rapid pericyte loss followed by reactive pericytosis in the cerebral cortex.
      Zehendner, CM; Sebastiani, A; Hugonnet, A; Bischoff, F; Luhmann, HJ; Thal, SC
      Scientific reports  5  13497  2015

      Show Abstract
      26333872 26333872
      Neuronal activity regulates remyelination via glutamate signalling to oligodendrocyte progenitors.
      Gautier, HO; Evans, KA; Volbracht, K; James, R; Sitnikov, S; Lundgaard, I; James, F; Lao-Peregrin, C; Reynolds, R; Franklin, RJ; Káradóttir, RT
      Nature communications  6  8518  2015

      Show Abstract
      26439639 26439639
      The adhesion G protein-coupled receptor GPR56 is a cell-autonomous regulator of oligodendrocyte development.
      Giera, S; Deng, Y; Luo, R; Ackerman, SD; Mogha, A; Monk, KR; Ying, Y; Jeong, SJ; Makinodan, M; Bialas, AR; Chang, BS; Stevens, B; Corfas, G; Piao, X
      Nature communications  6  6121  2015

      Show Abstract
      25607655 25607655
      Interneurons and oligodendrocyte progenitors form a structured synaptic network in the developing neocortex.
      Orduz, D; Maldonado, PP; Balia, M; Vélez-Fort, M; de Sars, V; Yanagawa, Y; Emiliani, V; Angulo, MC
      eLife  4  2015

      Show Abstract
      25902404 25902404
      Systemic inflammation in early neonatal mice induces transient and lasting neurodegenerative effects.
      Cardoso, FL; Herz, J; Fernandes, A; Rocha, J; Sepodes, B; Brito, MA; McGavern, DB; Brites, D
      Journal of neuroinflammation  12  82  2015

      Show Abstract
      25924675 25924675
      A Rac/Cdc42 exchange factor complex promotes formation of lateral filopodia and blood vessel lumen morphogenesis.
      Abraham, S; Scarcia, M; Bagshaw, RD; McMahon, K; Grant, G; Harvey, T; Yeo, M; Esteves, FO; Thygesen, HH; Jones, PF; Speirs, V; Hanby, AM; Selby, PJ; Lorger, M; Dear, TN; Pawson, T; Marshall, CJ; Mavria, G
      Nature communications  6  7286  2015

      Show Abstract
      26129894 26129894
      3-D Imaging Reveals Participation of Donor Islet Schwann Cells and Pericytes in Islet Transplantation and Graft Neurovascular Regeneration.
      Juang, JH; Kuo, CH; Peng, SJ; Tang, SC
      EBioMedicine  2  109-19  2015

      Show Abstract
      26137552 26137552
      Cytogenesis in the adult monkey motor cortex: perivascular NG2 cells are the major adult born cell type.
      Stanton, GB; Kohler, SJ; Boklweski, J; Cameron, JL; Greenough, WT
      The Journal of comparative neurology  523  849-68  2015

      Show Abstract
      25308320 25308320
      Dynamin 2 regulation of integrin endocytosis, but not VEGF signaling, is crucial for developmental angiogenesis.
      Lee, MY; Skoura, A; Park, EJ; Landskroner-Eiger, S; Jozsef, L; Luciano, AK; Murata, T; Pasula, S; Dong, Y; Bouaouina, M; Calderwood, DA; Ferguson, SM; De Camilli, P; Sessa, WC
      Development (Cambridge, England)  141  1465-72  2014

      Show Abstract
      24598168 24598168
      Endothelial Kruppel-like factor 4 regulates angiogenesis and the Notch signaling pathway.
      Hale, AT; Tian, H; Anih, E; Recio, FO; Shatat, MA; Johnson, T; Liao, X; Ramirez-Bergeron, DL; Proweller, A; Ishikawa, M; Hamik, A
      The Journal of biological chemistry  289  12016-28  2014

      Show Abstract
      24599951 24599951

      Brochure

      Title
      SNAP i.d. 2.0 System Brochure

      Technical Info

      Title
      Derivation of oligodendrocyte progenitor cells from a human neural stem cell line

      Data Sheet

      Title
      RABBIT ANTI-NG2 CHONDROITIN SULFATE PROTEOGLYCAN POLYCLONAL ANTIBODY

      Newsletters / Publications

      Title
      Cellutions - The newsletter for Cell Biology Researchers Volume 3: 2011

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies