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MABE956 | Anti-NFE2-related factor 1/Nrf1 Antibody, clone 17

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M WB R Purified Monoclonal Antibody
      Catalogue NumberMABE956
      DescriptionAnti-NFE2-related factor 1/Nrf1 Antibody, clone 17
      Alternate Names
      • Nuclear factor erythroid 2-related factor 1
      • NF-E2-related factor 1
      • NFE2-related factor 1
      • Nuclear factor, erythroid derived 2, like 1
      Background InformationNuclear factor erythroid 2-related factor 1 (NFE2-related factor 1/Nrf1), also known as NF-E2-related factor 1, NFE2-related factor 1, Nuclear factor erythroid derived 2 like 1, and encoded by the gene name Nfe2l1 and Nrf1, is a member of the bZIP family and the CNC subfamily. Isoform 3 of Nrf1 interacts with the extended kappa 3 site of the TNF alpha promoter after Fc gamma RIII stimulation and participates in the induction of this cytokine. Studies have suggested that isoform 1 of Nrf1 is either inactive or represses the transcriptional activation. Isoform 2 of Nrf1 has been shown to activate the expression of antioxidant response element (ARE) containing reporters and genes in cells, and may play a role in oxidative stress response. Other studies have suggested that isoform 1 also activates ARE-driven genes. Levels of Nrf1 isoform 2 in white fat, lung and liver are increased compared to isoform 1 (at protein level). Isoform 1 and isoform 2 of Nrf1 are widely expressed including kidney, brown fat, white fat, large intestine, small intestine, stomach, lung, brain and liver. Isoform 2 levels are elevated in brown fat and brain, but are reduced in liver compared to isoform 1 levels. Isoform 1 and isoform 2 are also expressed in mouse embryonic fibroblasts (MEF).
      Product Information
      PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationThis Anti-NFE2-related factor 1/Nrf1 antibody is validated for use in Western Blotting for the detection of NFE2-related factor 1/Nrf1.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected NFE2-related factor 1/Nrf1 in MEF cells stimulated with MG132 (Tsuchiya, Y., et al. (2011) Mole. Cell Biol. 31(22):4500-4512).
      Biological Information
      ImmunogenHis-tagged recombinant protein corresponding to mouse NFE2-related factor 1/Nrf1.
      ConcentrationPlease refer to lot specific datasheet.
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Nfe2l1
      • Nrf1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~85 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MG132 treated NIH-3T3 cell lysate.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected NFE2-related factor 1/Nrf1 in 10 µg of MG132 treated NIH-3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-NFE2-related factor 1/Nrf1, clone 17 -Q2485588 Q2485588
      Anti-c-Myc, clone 9E10 -2708956 2708956


      Reference overviewPub Med ID
      Dual regulation of the transcriptional activity of Nrf1 by β-TrCP- and Hrd1-dependent degradation mechanisms.
      Tsuchiya, Y; Morita, T; Kim, M; Iemura, S; Natsume, T; Yamamoto, M; Kobayashi, A
      Molecular and cellular biology 31 4500-12 2011

      Show Abstract
      21911472 21911472