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MAB5604 | Anti-NEDD9 Antibody

MAB5604
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R ICC, WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB5604
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-NEDD9 Antibody
      Alternate Names
      • Neural precursor cell expressed developmentally down-regulated 9
      • HEF1
      • CAS-L
      • p105
      References
      Product Information
      FormatPurified
      Control
      • Positive control: MCF7, HeLa and 3V1 cell lines. To date no staining has been seen on CHO cells.
      PresentationLiquid in 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 0.01% sodium azide.
      Applications
      ApplicationThis Anti-NEDD9 Antibody is validated for use in IC, WB for the detection of NEDD9.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesWestern blot: 1:1000 - 1:5,000. The antibody recognizes a doublet of ~105-115 kDa. The antibody may also show reactivity with the p55 isoform of Nedd9.

      Immunocytochemistry: 1:250-1:500 on 4% paraformaldehyde fixed cells.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide from human Nedd9.
      HostMouse
      SpecificityNedd9 (Neural precursor cell expressed developmentally down-regulated 9, HEF1, CAS-L, p105). The antibody does not recognize p130Cas.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NEDD9
      • dJ761I2.1
      • CasL
      • p105
      • CAS-L
      • dJ49G10.2
      • CASL
      • HEF1
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q14511 # Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form.
      SIZE: 834 amino acids; 92861 Da
      SUBUNIT: Interacts with BCAR3 and SH2D3C (By similarity). Homodimer. Can heterodimerize with HLH proteins ID2, E12, E47 and also with p130cas. Forms complexes in vivo with related adhesion focal tyrosine kinase (RAFTK), adapter protein CRKL and LYN kinase. Interacts with MICAL and TXNL4/DIM1.
      SUBCELLULAR LOCATION: Cytoplasm, cell cortex. Nucleus. Golgi apparatus. Cell projection, lamellipodium. Cytoplasm. Cell junction, focal adhesion. Note=Localizes to both the cell nucleus and the cell periphery and is differently localized in fibroblasts and epithelial cells. In fibroblasts is predominantly nuclear and in some cells is present in the Golgi apparatus. In epithelial cells localized predominantly in the cell periphery with particular concentration in lamellipodia but is also found in the nucleus. Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associates with mitotic spindle. & Enhancer of filamentation 1 p55: Spindle.
      TISSUE SPECIFICITY: Widely expressed. Higher levels detected in kidney, lung, and placenta. Also detected in T-cells, B-cells and diverse cell lines. The protein has been detected in lymphocytes, in diverse cell lines, and in lung tissues.
      DOMAIN: SwissProt: Q14511 Contains a central domain containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL SH2 domains. The HLH motif confers specific interaction with the HLH proteins ID2, E12 and E47. It is absolutely required for the induction of pseudohyphal growth in yeast and mediates homodimerization and heterodimerization with p130cas. & The SH3 domain interacts with two proline-rich regions of focal adhesion kinase.
      PTM: Cell cycle-regulated processing produces four isoforms: p115, p105, p65, and p55. Isoform p115 arises from p105 phosphorylation and appears later in the cell cycle. Isoform p55 arises from p105 as a result of cleavage at a caspase cleavage-related site and it appears specifically at mitosis. The p65 isoform is poorly detected. & Focal adhesion kinase 1 phosphorylates the protein at the YDYVHL motif (conserved among all cas proteins). The SRC family kinases (FYN, SRC, LCK and CRK) are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Ligation of either integrin beta-1 or B-cell antigen receptor on tonsillar B-cells and B-cell lines promotes tyrosine phosphorylation and both integrin and BCR-mediated tyrosine phosphorylation requires an intact actin network. In fibroblasts transformation with oncogene v-ABL results in an increase in tyrosine phosphorylation. Transiently phosphorylated following CD3 cross-linking and this phosphorylated form binds to CRK and C3G. A mutant lacking the SH3 domain is phosphorylated upon CD3 cross- linking but not upon integrin beta-1 cross-linking. Tyrosine phosphorylation occurs upon stimulation of the G-protein coupled C1a calcitonin receptor in rabbit. Calcitonin-stimulated tyrosine phosphorylation is mediated by calcium- and protein kinase C- dependent mechanisms and requires the integrity of the actin cytoskeleton.DISEASE:SwissProt: Q14511 # May play a role in pathways leading to progression of cancer.
      SIMILARITY: Belongs to the CAS family. & Contains 1 SH3 domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies