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MABS10 | Anti-NEDD8-conjugating enzyme Ubc12 Antibody, clone 9H1.1

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H WB, ICC M Purified Monoclonal Antibody
      Catalogue NumberMABS10
      DescriptionAnti-NEDD8-conjugating enzyme Ubc12 Antibody, clone 9H1.1
      Alternate Names
      • neural precursor cell expressed
      • NEDD8 carrier protein
      • NEDD8 protein ligase
      • Ubiquitin-conjugating enzyme E2 M
      Background InformationNEDD8-conjugating enzyme Ubc12, an E2 enzyme necessary for NEDDylation, has been shown to form a thioester linkage with the ubiquitin like protein NEDD8 in the presence of the NEDD8 activating enzyme complex Uba3/APP-BP1. The NEDD8 pathway is essential for cell cycle progression and morphogenesis.
      Product Information
      • NIH/3T3 cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-NEDD8-conjugating enzyme Ubc12 Antibody, clone 9H1.1 detects level of NEDD8-conjugating enzyme Ubc12 & has been published & validated for use in WB & IC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: 1:500 dilution from a previous lot detected NEDD8-conjugating enzyme Ubc12 in NIH/3T3, A431, and HeLa cells.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human NEDD8-conjugating enzyme Ubc12.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Mouse
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq].
      Gene Symbol
      • UBE2M
      • UBC12
      Purification MethodProtein G
      UniProt Number
      UniProt SummaryFUNCTION: Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.

      CATALYTIC ACTIVITY: ATP + NEDD8 + protein lysine = AMP + diphosphate + protein N-NEDD8yllysine.

      PATHWAY: Protein modification; protein neddylation.

      SUBUNIT STRUCTURE: Interacts with UBA3 and RBX1.

      DOMAIN: Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.

      SEQUENCE SIMILARITIES: Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
      Molecular Weight ~ 21 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in NIH/3T3 cell lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected NEDD8-conjugating enzyme Ubc12 on 10 µg of NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-NEDD8-conjugating enzyme Ubc12, clone 9H1.1 - NRG1818957 NRG1818957


      Reference overviewPub Med ID
      Semen alloantigens and lymphocytotoxic antibodies in AIDS and ICL.
      R S Root-Bernstein,S H DeWitt
      Genetica 95 1995

      Show Abstract
      7744257 7744257
      AIDS dementia complex complicated by cytomegalovirus encephalopathy.
      M Fiala,E J Singer,M C Graves,W W Tourtellotte,J A Stewart,C A Schable,R H Rhodes,H V Vinters
      Journal of neurology 240 1993

      Show Abstract
      8388434 8388434