AP1160 Anti-NDRG1 Mouse mAb (2D7)

AP1160
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      AP1160-100UG
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          100 μg
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          Description
          OverviewRecognizes the ~40-45 kDa NDRG1 protein in HeLa cells and human endometrium tissue.
          Catalogue NumberAP1160
          Brand Family Calbiochem®
          SynonymsAnti-N-myc Downstream Regulator 1
          Application Data
          Detection of human NDRG1 by immunoblotting. Sample: Full-length recombinant human NDRG1 protein. Primary antibody: Anti-NDRG1 Mouse mAb (2D7) (Cat. No. AP1160) (1-5 µg/ml). Detection: chemiluniescence.

          Detection of human NDRG1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-NDRG1 Mouse mAb (2D7) (Cat. No. AP1160) (10 µg/ml). Detection: fluorescence.

          Detection of human NDRG1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human endometrium. Primary antibody: Anti-NDRG1 Mouse mAb (2D7) (Cat. No. AP1160) (0.7 µg/ml). Detection: colorimetric (DAB).
          References
          ReferencesJiang, K., et al. 2010. Scand. J. Gastroenterol 45, 898.
          Leth-Larsen R, et al. 2009. Mol. Cell Proteomics 8, 1436.
          Product Information
          FormLiquid
          FormulationIn PBS, pH 7.2.
          Positive controlHuman endometrium tissue, HeLa cells
          PreservativeNone
          Applications
          Key Applications Paraffin Sections
          Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Application NotesELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Immunocytochemistry (10 µg/ml)
          Paraffin Sections (0.7 µg/ml)
          Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenFull-length, recombinant human NDRG1 (aa 1-395), expressed as a GST fusion protein
          Clone2D7
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-NDRG1 Mouse mAb (2D7) SDS

          Title

          Safety Data Sheet (SDS) 

          References

          Reference overview
          Jiang, K., et al. 2010. Scand. J. Gastroenterol 45, 898.
          Leth-Larsen R, et al. 2009. Mol. Cell Proteomics 8, 1436.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision19-October-2010 RFH
          SynonymsAnti-N-myc Downstream Regulator 1
          ApplicationELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Immunocytochemistry (10 µg/ml)
          Paraffin Sections (0.7 µg/ml)
          Application Data
          Detection of human NDRG1 by immunoblotting. Sample: Full-length recombinant human NDRG1 protein. Primary antibody: Anti-NDRG1 Mouse mAb (2D7) (Cat. No. AP1160) (1-5 µg/ml). Detection: chemiluniescence.

          Detection of human NDRG1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-NDRG1 Mouse mAb (2D7) (Cat. No. AP1160) (10 µg/ml). Detection: fluorescence.

          Detection of human NDRG1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human endometrium. Primary antibody: Anti-NDRG1 Mouse mAb (2D7) (Cat. No. AP1160) (0.7 µg/ml). Detection: colorimetric (DAB).
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~40-45 kDa NDRG1 protein.
          BackgroundNDRG1 is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. It is necessary for p53-mediated caspase activation and apoptosis. Mutation in this gene has been reported to be causative for hereditary motor and sensory neuropathy-Lom.
          HostMouse
          ImmunogenFull-length, recombinant human NDRG1 (aa 1-395), expressed as a GST fusion protein
          Clone2D7
          IsotypeIgG2a
          Specieshuman
          Positive controlHuman endometrium tissue, HeLa cells
          FormLiquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Regulatory Review
          ReferencesJiang, K., et al. 2010. Scand. J. Gastroenterol 45, 898.
          Leth-Larsen R, et al. 2009. Mol. Cell Proteomics 8, 1436.