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MAB4386 | Anti-NDP52 Antibody, clone 10F1.2

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M WB M Purified Monoclonal Antibody
      Catalogue NumberMAB4386
      DescriptionAnti-NDP52 Antibody, clone 10F1.2
      Alternate Names
      • Antigen nuclear dot 52 kDa protein
      • Nuclear domain 10 protein 52
      • Nuclear domain 10 protein NDP52
      • Nuclear dot protein 52
      • calcium binding and coiled-coil domain 2
      • nuclear domain 10 protein
      Background InformationNDP52, also known as Nuclear Domain 10 protein NDP52 or Calcium-binding and coiled-coil domain-containing protein 2, is a novel protein of the ND10 (nuclear domain 10). It is part of a complex consisting of CALCOCO2, TAX1BP1 and MYO6 and interacts with GEMIN4. NDP52 expression is limited to the pluripotent cells of the early embryo and the germline. It is also expressed in blastocysts, epiblasts and purified primordial germ cells. Recent findings show that NDP52 co-localizes with the ND10 protein PML and can be redistributed upon viral infection and interferon treatment, suggesting that ND10 proteins play an important role in the viral life cycle. Furthermore, NPD252 may play a role in ruffle formation and actin cytoskeleton organization.
      Product Information
      • Mouse embryonic stem cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      ApplicationThis Anti-NDP52 Antibody, clone 10F1.2 is validated for use in WB for the detection of NDP52.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to mouse NDP52.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes NDP52.
      Species Reactivity
      • Mouse
      Species Reactivity NoteProven to react with mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NDP52
      • CALCOCO2
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: May play a role in ruffle formation and actin cytoskeleton organization. Seems to negatively regulate constitutive secretion By similarity.

      SUBUNIT STRUCTURE: Part of a complex consisting of CALCOCO2, TAX1BP1 and MYO6. Interacts with GEMIN4 By similarity.

      SUBCELLULAR LOCATION: Cytoplasm › perinuclear region By similarity. Golgi apparatus By similarity. Cytoplasm › cytoskeleton By similarity.

      DEVELOPMENTAL STAGE: Expression is limited to the pluripotent cells of the early embryo and the germline. Expressed in blastocysts, epiblasts and purified primordial germ cells.

      SEQUENCE SIMLARITIES: Belongs to the CALCOCO family.

      SEQUENCE CAUTION: The sequence BAE38997.1 differs from that shown. Reason: Frameshift at position 193.
      Molecular Weight~40 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse embryonic stem cell lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected NDP52 in 10 µg of mouse embryonic stem cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-NDP52, clone 10F1.2 - 2119007 2119007
      Anti-NDP52, clone 10F1.2 - 2360878 2360878
      Anti-NDP52, clone 10F1.2 - 2450175 2450175
      Anti-NDP52, clone 10F1.2 - 2172758 2172758
      Anti-NDP52, clone 10F1.2 - NG1944982 NG1944982
      Anti-NDP52, clone 10F1.2 - NRG1665144 NRG1665144


      Reference overviewPub Med ID
      Depletion of the ubiquitin-binding adaptor molecule SQSTM1/p62 from macrophages harboring cftr ΔF508 mutation improves the delivery of Burkholderia cenocepacia to the autophagic machinery.
      Abdulrahman, BA; Khweek, AA; Akhter, A; Caution, K; Tazi, M; Hassan, H; Zhang, Y; Rowland, PD; Malhotra, S; Aeffner, F; Davis, IC; Valvano, MA; Amer, AO
      The Journal of biological chemistry 288 2049-58 2013

      Show Abstract
      23148214 23148214