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ABD135 | Anti-NANOS3 Antibody

ABD135
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IH(P), IF Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberABD135
      DescriptionAnti-NANOS3 Antibody
      Alternate Names
      • Nanos homolog 3
      • NOS-3
      • NOS3
      Background InformationNanos homolog 3 (Nanos3), also known as NOS-3 or NOS3, belongs to the Nanos family. Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. Nanos3 has been shown to play an important role in the maintenance of the undifferentiated state of germ cells regulating the spermatogonia cell cycle and inducing a prolonged transit in G1 phase. Nanos3 has also been shown to be essential in the early stage embryo protecting the migrating primordial germ cells (PGCs) from apoptosis. Additionally, it is implied to maintain the germ cell lineage by suppressing both Bax-dependent and -independent apoptotic pathways and to affect cell proliferation by repressing translation of specific mRNAs. Nanos3 is expressed primarily in ovary, testis and brain. In the ovaries, Nanos3 is expressed during multiple stages of oogenesis, including primordial, primary, secondary and antral follicles with the highest expression in the oocytes. In the testis, Nonos3 is expressed in germ cells, type A spermatogonia (SA), primary spermatocytes (S1), round spermatids (S3) and elongated spermatids.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Human brain tissue lysate
      PresentationPurified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
      Applications
      ApplicationDetect Nanos homolog 3 using this rabbit polyclonal antibody, Anti-NANOS3 Antibody validated for use in western blotting, IHC (Paraffin) & Immunofluorescence.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunofluorescence
      Application NotesImmunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected NANOS3 in human brain tissue.

      Immunofluorescence Analysis: 20 μg/mL from a representative lot detected NANOS3 in human brain cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human NANOS3.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes the C-terminus of NANOS3.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • NANOS3
      • Nanos3
      Purification MethodAntigen Affinity Purified
      UniProt Number
      Molecular Weight~21 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 2 isoforms at ~19 kDa and ~21 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human brain tissue lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected NANOS3 in 15 µg of human brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications