A part of MilliporeSigma

MABE333 | Anti-N-Myc Antibody, clone NCM II 100

100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers


      Contact Customer Service

      Click To Print This Page


      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IP, ICC M Purified Monoclonal Antibody
      Catalogue NumberMABE333
      DescriptionAnti-N-Myc Antibody, clone NCM II 100
      Alternate Names
      • N-myc proto-oncogene protein
      • Class E basic helix-loop-helix protein 37
      • bHLHe37
      Background InformationThe v-myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans the family consists of five genes: c-myc, N-myc, R-myc, L-myc and B-myc. Amplification of the N-myc gene has been found in human neuroblastomas and cell lines. The extent of N-myc amplification correlates well with the stage of neuroblastoma disease. Immunological studies have shown that the human N-myc gene gives rise to at least two nuclear phosphoproteins that exhibit relatively short (30 min) half lives in vivo and exhibit DNA binding properties in vitro.
      Product Information
      • IMR-32 cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationUse Anti-N-Myc Antibody, clone NCM II 100 (mouse monoclonal antibody) validated in WB, IP, ICC to detect N-Myc also known as N-myc proto-oncogene protein, Class E basic helix-loop-helix protein 37, bHLHe37.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesImmunofluorescence Analysis: A representative lot from an independent laboratory detected N-Myc in IMR5 cells (Ikegaki, N., et al. (1986). 83(16):5929-5933.).

      Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated N-Myc in IP (Brondyk, W. H., et al. (1991). 6(7):1269-1276.).
      Biological Information
      ImmunogenRecombinant protein corresponding to human N-Myc.
      CloneNCM II 100
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MYCN
      • BHLHE37
      • NMYC
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~55 kDa observed. Uniprot describes a molecular weight at ~50 kDa. Uniprot states that this protein may be phosphorylated.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in IMR-32 cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected N-Myc in 10 µg of IMR-32 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-N-Myc, clone NCM II 100 - 2433595 2433595
      Anti-N-Myc, clone NCM II 100 - Q2013511 Q2013511
      Anti-N-Myc, clone NCM II 100 -2638108 2638108
      Anti-N-Myc, clone NCM II 100 -2676716 2676716
      Anti-N-Myc, clone NCM II 100 -2731008 2731008


      Reference overviewPub Med ID
      N-myc oncogene enhances mitogenic responsiveness of diploid human fibroblasts to growth factors but fails to immortalize.
      Brondyk, W H, et al.
      Oncogene, 6: 1269-76 (1991) 1991

      Show Abstract
      1861869 1861869
      Identification and characterization of the NMYC gene product in human neuroblastoma cells by monoclonal antibodies with defined specificities.
      Ikegaki, N, et al.
      Proc. Natl. Acad. Sci. U.S.A., 83: 5929-33 (1986) 1986

      Show Abstract
      2426708 2426708


      New Products: Volume 3, 2012

      Related Products & Applications

      Related Products

      Catalog Number Description  
      AP124P Goat Anti-Mouse IgG Antibody, Peroxidase Conjugated, H+L Show Pricing & Availability
      CBL431 Anti-N-Myc Antibody, clone IA8 Show Pricing & Availability

      View Gene Related Products for this Target


      Life Science Research > Antibodies and Assays > Primary Antibodies