MAB382 Anti-Myelin Basic Protein Antibody, a.a. 129-138, clone 1

MAB382
2 mL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, R ELISA, IHC, IH(P), RIA, WB M Culture Supernatant Monoclonal Antibody
      Description
      Catalogue NumberMAB382
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Myelin Basic Protein Antibody, a.a. 129-138, clone 1
      Alternate Names
      • Myelin A1 protein
      • Myelin membrane encephalitogenic protein
      • myelin basic protein
      Background InformationThe classic group of MBP isoforms (isoforms 4-14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoforms 1-3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined to optional posttranslational modifications give a wide spectrum of isomers, each of them having maybe a specialized function.

      MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.

      Isoform 1 Golli-MBP1, HOG7, 33 kDa

      Isoform 2 Golli-MBP2, HOG5, 21.5 kDa

      Isoform 3 MBP1, 21.5 kDa

      Isoform 4 MBP2, 20.2 kDa

      Isoform 5 MBP3, 18.5 kDa

      Isoform 6 MBP4, 17.2 kDa

      (SP_P02686)
      References
      Product Information
      FormatCulture Supernatant
      Control
      • Brain tissue
      PresentationCulture supernatant containing 0.2 M Tris/HCl, pH 7.4 with 5-10% fetal calf serum and 0.1% sodium azide
      Applications
      ApplicationThis Anti-Myelin Basic Protein Antibody, a.a. 129-138, clone 1 is validated for use in ELISA, IH, IH(P), RIA, WB for the detection of Myelin Basic Protein.
      Key Applications
      • ELISA
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Radioimmunoassay
      • Western Blotting
      Application NotesImmunohistochemistry(paraffin) Analysis:
      Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum
      Immunohistology on frozen sections at 1:10

      Western Blot Analysis:
      A previous lot of this antibody was used in Western Blot.

      ELISA:
      A 1:200-1:1,000 dilution of a previous lot was used in ELISA.

      RIA:
      A previous lot of this antibody was used in Radioimmunoassay.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenBovine myelin basic protein
      Epitopea.a. 129-138
      Clone1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with MBP from human, bovine and rat, epitope 129-138
      IsotypeIgG2a
      Species Reactivity
      • Bovine
      • Human
      • Rat
      Species Reactivity NoteReacts weakly with rabbit. Does not react with guinea pig.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.
      Gene Symbol
      • MBP
      • MGC99675
      • Golli-mbp
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P02686 # The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non- classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T- cells and neural cells. Differential splicing events combined to optional post-translational modifications give a wide spectrum of isomers, each of them having maybe a specialized function. Induces T-cell proliferation.
      SIZE: 304 amino acids; 33117 Da
      SUBUNIT: Homodimer; isoform 3 exists as a homodimer.
      SUBCELLULAR LOCATION: Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic side of myelin.
      TISSUE SPECIFICITY: MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.DEVELOPMENTAL STAGE: Expression turns on abruptly in fetus of 14 to 16 weeks. Even smaller isoforms seem to be produced during embryogenesis, some of these persisting in the adult. Expression of isoform MBP2 is more evident at 16 weeks and its relative proportion declined thereafter.
      PTM: Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases in aging, making the protein more cationic. & The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6). & Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
      DISEASE: SwissProt: P02686 # The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
      SIMILARITY: SwissProt: P02686 ## Belongs to the myelin basic protein family.
      Molecular Weight19 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunohistochemistry on brain tissue.

      Immunohistochemistry(paraffin) Analysis:
      MBP (cat. # MAB382) staining pattern/morphology in rat cerebellum. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:50, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seen as fiber staining in the junction between granular layer and molecular layer.
      Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC in undiluted aliquots from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size2 mL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Myelin Basic Protein, a.a. 129 138, clone 13016053
      Anti-Myelin Basic Protein, a.a. 129- -25490102549010
      Anti-Myelin Basic Protein, a.a. 129- -26891542689154
      Anti-Myelin Basic Protein, a.a. 129- -28254322825432
      Anti-Myelin Basic Protein, a.a. 129- 138, clone 1 - 19690931969093
      Anti-Myelin Basic Protein, a.a. 129- 138, clone 1 - 20350562035056
      Anti-Myelin Basic Protein, a.a. 129- 138, clone 1 - 20539222053922
      Anti-Myelin Basic Protein, a.a. 129- 138, clone 1 - JBC1780786JBC1780786
      Anti-Myelin Basic Protein, a.a. 129- 138, clone 1 - JC1629915JC1629915
      Anti-Myelin Basic Protein, a.a. 129- 138, clone 1 - JC1658203JC1658203

      References

      Reference overviewApplicationSpeciesPub Med ID
      Neuroprotective effects of activated protein C on intrauterine inflammation-induced neonatal white matter injury are associated with the downregulation of fibrinogen-like protein 2/fibroleukin prothrombinase and the inhibition of pro-inflammatory cytokine expression.
      Jin, SJ; Liu, Y; Deng, SH; Liao, LH; Lin, TL; Ning, Q; Luo, XP
      International journal of molecular medicine  35  1199-212  2015

      Show Abstract
      25777531 25777531
      Genetic labeling reveals novel cellular targets of schizophrenia susceptibility gene: distribution of GABA and non-GABA ErbB4-positive cells in adult mouse brain.
      Bean, JC; Lin, TW; Sathyamurthy, A; Liu, F; Yin, DM; Xiong, WC; Mei, L
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  13549-66  2014

      Show Abstract
      ImmunohistochemistryMouse25274830 25274830
      Long-term characterization of axon regeneration and matrix changes using multiple channel bridges for spinal cord regeneration.
      Tuinstra, HM; Margul, DJ; Goodman, AG; Boehler, RM; Holland, SJ; Zelivyanskaya, ML; Cummings, BJ; Anderson, AJ; Shea, LD
      Tissue engineering. Part A  20  1027-37  2014

      Show Abstract
      Immunofluorescence24168314 24168314
      Natural and lesion-induced decrease in cell proliferation in the medial nucleus of the trapezoid body during hearing development.
      Saliu, A; Adise, S; Xian, S; Kudelska, K; Rodríguez-Contreras, A
      The Journal of comparative neurology  522  971-85  2014

      Show Abstract
      24115041 24115041
      Nucleus-localized 21.5-kDa myelin basic protein promotes oligodendrocyte proliferation and enhances neurite outgrowth in coculture, unlike the plasma membrane-associated 18.5-kDa isoform.
      Smith, GS; Samborska, B; Hawley, SP; Klaiman, JM; Gillis, TE; Jones, N; Boggs, JM; Harauz, G
      Journal of neuroscience research  91  349-62  2013

      Show Abstract
      23184356 23184356
      Hyccin, the molecule mutated in the leukodystrophy hypomyelination and congenital cataract (HCC), is a neuronal protein.
      Gazzerro, E; Baldassari, S; Giacomini, C; Musante, V; Fruscione, F; La Padula, V; Biancheri, R; Scarfì, S; Prada, V; Sotgia, F; Duncan, ID; Zara, F; Werner, HB; Lisanti, MP; Nobbio, L; Corradi, A; Minetti, C
      PloS one  7  e32180  2012

      Show Abstract
      22461884 22461884
      Isolation rearing in rats: Effect on expression of synaptic, myelin and GABA-related immunoreactivity and its utility for drug screening via the subchronic parenteral route.
      Lim AL, Taylor DA, Malone DT
      Brain Res  2011

      Show Abstract
      21241674 21241674
      Sphingosine kinase 1 and sphingosine 1-phosphate receptor 3 are functionally upregulated on astrocytes under pro-inflammatory conditions.
      Fischer, I; Alliod, C; Martinier, N; Newcombe, J; Brana, C; Pouly, S
      PloS one  6  e23905  2011

      Show Abstract
      21887342 21887342
      Transient degradation of myelin basic protein in the rat hippocampus following acute carbon monoxide poisoning.
      Satoru Watanabe,Hirotaka Matsuo,Yasushi Kobayashi,Yasushi Satoh,Masanori Fujita,Akiyoshi Nakayama,Yoshifusa Aizawa,Nariyoshi Shinomiya,Shinya Suzuki
      Neuroscience research  68  2010

      Show Abstract
      20633582 20633582
      Involvement of Notch1 inhibition in serum-stimulated glia and oligodendrocyte differentiation from human mesenchymal stem cells.
      Lee, YJ; Hung, SC; Chu, MS
      Stem cells and cloning : advances and applications  3  165-73  2010

      Show Abstract
      Western Blotting24198522 24198522

      Data Sheet

      Title
      Anti-Myelin Basic Protein, a.a. 129-138, clone 1 - Data Sheet

      Related Products & Applications

      Related Products

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies