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MAB4307 | Anti-Mouse Leukemia Inhibitory Factor Antibody, clone 2H2.2

MAB4307
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M ELISA, WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB4307
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Mouse Leukemia Inhibitory Factor Antibody, clone 2H2.2
      Alternate Names
      • mLIF
      Background InformationLeukemia inhibitory factor, or LIF, is a pleiotropic cytokine that is expressed by a wide variety of cells including activated T lymphocytes, monocytes, mast cells and neuronal cells. Human LIF cDNA encodes a 202 amino acid precursor which is processed to yield a 180 amino acid, biologically active glycoprotein with an approximate molecular weight of 38-67 kDa. In addition to its involvement in the stimulation of acute-phase protein during inflammatory processes, LIF has also been implicated in promoting the survival and growth of axons in vitro. LIF signals through the LIF receptor, a heterodimer comprised of a 190 kDa LIFR subunit and the gp130 subunit common to the IL-6, IL-11, CNTF and OSM receptors. Janus tyrosine kinases, JAK1 and JAK2, are activated in response to LIF stimulation, which in turn phosphorylate Stat1 and Stat3 causing their nuclear translocation and the transcription of Stat-responsive genes.
      References
      Product Information
      FormatPurified
      Control
      • Mouse DA-1a cells
      PresentationPurified Immunoglobulin. Liquid in 0.02M PBS, 0.25M NaCl ph 7.6 with 0.1% Sodium Azide.
      Applications
      ApplicationAnti-Mouse Leukemia Inhibitory Factor Antibody, clone 2H2.2 is an antibody against Mouse Leukemia Inhibitory Factor for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesImmunoblotting: a starting dilution of 1-10 μg/mL is suggested.

      EIA

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRecombinant Murine LIF.
      Clone2H2.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody is reactive to E.coli and STO derived recombinant murine Leukemia Inhibitory Factor (LIF).
      IsotypeIgG1
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene SummaryLeukaemia inhibitory factor is a cytokine that induces macrophage differentiation. Neurotransmitters and neuropeptides, well known for their role in the communication between neurons, are also capable of activating monocytes and macrophages and inducing chemotaxis in immune cells. LIF signals through different receptors and transcription factors. LIF in conjunction with BMP2 acts in synergy on primary fetal neural progenitor cells to induce astrocytes.
      Gene Symbol
      • LIF
      • HILDA
      • MLPLI
      • CDF
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P15018 # LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
      SIZE: 202 amino acids; 22008 Da
      SUBCELLULAR LOCATION: Secreted.
      SIMILARITY: SwissProt: P15018 ## Belongs to the LIF/OSM family.
      Stem Cell Type
      • Hematopoietic Stem Cells
      • Neural Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI MURINE LIF MONOCLONAL ANTIBODY - 2395709 2395709
      MOUSE ANTI MURINE LIF MONOCLONAL ANTIBODY - 2459539 2459539
      MOUSE ANTI MURINE LIF MONOCLONAL ANTIBODY -2581297 2581297
      MOUSE ANTI MURINE LIF MONOCLONAL ANTIBODY -2750276 2750276
      MOUSE ANTI MURINE LIF MONOCLONAL ANTIBODY -2762244 2762244
      MOUSE ANTI MURINE LIF MONOCLONAL ANTIBODY_2826585 2826585

      References

      Reference overviewPub Med ID
      In vitro expansion of a multipotent population of human neural progenitor cells.
      Carpenter, M K, et al.
      Exp. Neurol., 158: 265-78 (1999) 1999

      Show Abstract
      10415135 10415135
      In vivo and in vitro regulation of thyroid leukemia inhibitory factor (LIF): marker of hypothyroidism.
      Ren, S G, et al.
      J. Clin. Endocrinol. Metab., 84: 2883-7 (1999) 1999

      Show Abstract
      10443695 10443695
      Leukemia inhibitory factor, glial cell line-derived neurotrophic factor, and their receptor expressions following muscle crush injury.
      Kami, K, et al.
      Muscle Nerve, 22: 1576-86 (1999) 1999

      Show Abstract
      10514237 10514237
      Review: The role of leukaemia inhibitory factor in the establishment of pregnancy.
      Vogiagis, D and Salamonsen, L A
      J. Endocrinol., 160: 181-90 (1999) 1999

      Show Abstract
      9924186 9924186

      Data Sheet

      Title
      Anti-Mouse Leukemia Inhibitory Factor, clone 2H2.2 - Data Sheet