MAB1273 Anti-Mitochondria Antibody, surface of intact mitochondria, clone 113-1

MAB1273
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H IF, IHC, IH(P), IP M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB1273
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Mitochondria Antibody, surface of intact mitochondria, clone 113-1
      Background InformationThis antibody recognizes a 60 kDa nonglycosylated protein component of mitochondria found in human cells. It can be used to stain mitochondria in cell, tissue or biochemical preparations and can be used as a marker of the mitochondria in subcellular fractions.
      References
      Product Information
      FormatPurified
      Control
      • COS7 cells.
      PresentationPurified mouse monoclonal IgG1 in buffer containing liquid in PBS. Contains no preservative. Concentrated hybridoma supernatant.
      Applications
      ApplicationAnti-Mitochondria Antibody, surface of intact mitochondria, clone 113-1 is an antibody against Mitochondria for use in IF, IH, IH(P) & IP.
      Key Applications
      • Immunofluorescence
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      Application NotesImmunohistochemistry:
      1:100 dilutoin from a previous lot was used. Works best with organic fixatives (acetone, methanol, etc). 2% PFA for 10-15 min can be used but stronger use of formalin or 4% PFA limits staining.
      The antibody has been used to isolate intact mitochondria from freeze/thaw lysates of living cells via conjugation to magnetic beads {http://www.dynalbiotech.com}.

      Immunohistochemistry in paraffin embedded tissues:
      Requires citric acid/microwave antigen retrieval; enhanced ABC or amplified detection systems recommended.
      Optimal Staining With EDTA Buffer, pH 8.0, Epitope Retrieval: Heart Ventricle Epithelium

      The antibody has been used to isolate intact mitochondria from freeze/thaw lysates of living cells via conjugation to magnetic beads {http://www.dynalbiotech.com}.

      Indirect immunofluorescence:
      1:10-1:50 dilution from a previous lot was used, 30 - 100 μL per slide well.

      MAB1273 recognizes a 65 kD protein by immunoprecipitation.

      Optimal working dilutions and protocols must be determined by the end user.
      Biological Information
      Epitopesurface of intact mitochondria
      Clone113-1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityGives mitochondrial staining on all human cell types and does not cross react with rat and mouse tissue. The staining pattern is best described as a spaghetti-like staining pattern in fibroblasts and a perinuclear large speckled pattern in lymphonoid cells.
      MAB1273 recognizes a 65 kD protein by immunoprecipitation. Antibody produces the stringy, "spaghetti-like" staining patten in the cytoplasm of human cells.
      IsotypeIgG1
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Not rat or mouse. Reactivity with other species has not been determined
      Antibody TypeMonoclonal Antibody
      Purification MethodAmmonium Sulfate Precipitation
      Stem Cell Type
      • Neural Stem Cells
      Molecular Weight65 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunohistochemistry on heart ventricle cells.

      Immunohistochemistry(paraffin) Analysis:
      Mitochondria (cat. # MAB1273) staining on heart ventricle cells. Tissue pretreated with EDTA, pH 8.0This lot of antibody was diluted to 1:80, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seeing the circular-granular layer surrounding the nucleus of the cells.
      Optimal Staining With EDTA Buffer, pH 8.0, Epitope Retrieval: Heart Ventricle Epithelium
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at -20°C in undiluted aliquots from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Mitochondria Antibody, surface of intact mitochondria, clone 113-1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Mitochondria Antibody, surface of intact mitochondria, clone 113-1 Certificates of Analysis

      TitleLot Number
      Anti-Mitochondria, surface of intact -27228602722860
      Anti-Mitochondria, surface of intact mitochondria, clone 113-12484154
      Anti-Mitochondria, surface of intact mitochondria, clone 113-1 - 21482262148226
      Anti-Mitochondria, surface of intact mitochondria, clone 113-1 - 24303322430332
      Anti-Mitochondria, surface of intact mitochondria, clone 113-1 - 19973601997360
      Anti-Mitochondria, surface of intact mitochondria, clone 113-1 - 20211762021176
      Anti-Mitochondria, surface of intact mitochondria, clone 113-1 - 20659602065960
      Anti-Mitochondria, surface of intact mitochondria, clone 113-1 - 23245032324503
      Anti-Mitochondria, surface of intact mitochondria, clone 113-1 - 24939262493926
      Anti-Mitochondria, surface of intact mitochondria, clone 113-1 - JBC1766811JBC1766811

      References

      Reference overviewApplicationSpeciesPub Med ID
      A Site-Specific Integrated Col2.3GFP Reporter Identifies Osteoblasts Within Mineralized Tissue Formed In Vivo by Human Embryonic Stem Cells.
      Xin, X; Jiang, X; Wang, L; Stover, ML; Zhan, S; Huang, J; Goldberg, AJ; Liu, Y; Kuhn, L; Reichenberger, EJ; Rowe, DW; Lichtler, AC
      Stem cells translational medicine  3  1125-37  2014

      Show Abstract
      25122686 25122686
      Homing of neural stem cells from the venous compartment into a brain infarct does not involve conventional interactions with vascular endothelium.
      Goncharova, V; Das, S; Niles, W; Schraufstatter, I; Wong, AK; Povaly, T; Wakeman, D; Miller, L; Snyder, EY; Khaldoyanidi, SK
      Stem cells translational medicine  3  229-40  2014

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      24396034 24396034
      Role of mesenchymal stem cells in osteosarcoma and metabolic reprogramming of tumor cells.
      Bonuccelli, G; Avnet, S; Grisendi, G; Salerno, M; Granchi, D; Dominici, M; Kusuzaki, K; Baldini, N
      Oncotarget  5  7575-88  2014

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      25277190 25277190
      Developmental-like bone regeneration by human embryonic stem cell-derived mesenchymal cells.
      Kuhn, LT; Liu, Y; Boyd, NL; Dennis, JE; Jiang, X; Xin, X; Charles, LF; Wang, L; Aguila, HL; Rowe, DW; Lichtler, AC; Goldberg, AJ
      Tissue engineering. Part A  20  365-77  2014

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      Magnetic resonance imaging tracking of ferumoxytol-labeled human neural stem cells: studies leading to clinical use.
      Gutova, M; Frank, JA; D'Apuzzo, M; Khankaldyyan, V; Gilchrist, MM; Annala, AJ; Metz, MZ; Abramyants, Y; Herrmann, KA; Ghoda, LY; Najbauer, J; Brown, CE; Blanchard, MS; Lesniak, MS; Kim, SU; Barish, ME; Aboody, KS; Moats, RA
      Stem cells translational medicine  2  766-75  2013

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      24014682 24014682
      Neural progenitors derived from human induced pluripotent stem cells survive and differentiate upon transplantation into a rat model of amyotrophic lateral sclerosis.
      Popescu, IR; Nicaise, C; Liu, S; Bisch, G; Knippenberg, S; Daubie, V; Bohl, D; Pochet, R
      Stem cells translational medicine  2  167-74  2013

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      Platelet-rich plasma promotes the proliferation of human muscle derived progenitor cells and maintains their stemness.
      Li, H; Usas, A; Poddar, M; Chen, CW; Thompson, S; Ahani, B; Cummins, J; Lavasani, M; Huard, J
      PloS one  8  e64923  2013

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      Origin of the vasculature supporting growth of primary patient tumor xenografts.
      Hylander, BL; Punt, N; Tang, H; Hillman, J; Vaughan, M; Bshara, W; Pitoniak, R; Repasky, EA
      Journal of translational medicine  11  110  2013

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      Functional transplantation of salivary gland cells differentiated from mouse early ES cells in vitro.
      Kawakami, M; Ishikawa, H; Tachibana, T; Tanaka, A; Mataga, I
      Human cell  26  80-90  2013

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      23681939 23681939
      Ketone bodies and two-compartment tumor metabolism: stromal ketone production fuels mitochondrial biogenesis in epithelial cancer cells.
      Martinez-Outschoorn, UE; Lin, Z; Whitaker-Menezes, D; Howell, A; Lisanti, MP; Sotgia, F
      Cell cycle (Georgetown, Tex.)  11  3956-63  2012

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      23082721 23082721