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MABE920 | Anti-Mi-2β Antibody, clone 17H11

MABE920
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, ChIP, ChIP-seq M Affinity Purified Monoclonal Antibody
      Description
      Catalogue NumberMABE920
      DescriptionAnti-Mi-2β Antibody, clone 17H11
      Alternate Names
      • Chromodomain-helicase-DNA-binding protein 4
      • CHD-4
      • ATP-dependent helicase CHD4
      • Mi-2 autoantigen 218 kDa protein
      • Mi2-beta
      • Mi-2b
      Background InformationCHD-4, often referred to as Mi2-beta, is a central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. CHD-4 has been shown to interact with TRIM27, HDAC1, Histone deacetylase 2, MTA2, SATB1 and Ataxia telangiectasia and Rad3 related. This protein also interacts with KLF1. Sumoylation of KLF1 leads to transcriptional repression of CHD-4.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified mouse monoclonal IgG2bκ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-Mi-2β Antibody, clone 17H11 is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of Mi-2β.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      • ChIP-seq
      Application NotesChromatin Immunoprecipitation (ChIP): A representative lot of this antibody as part of a monoclonal antibody cocktail was used for ChIP. See Zhang et al., 2012 for details.

      ChIP-seq Analysis: A representative lot of this antibody as part of a monoclonal antibody cocktail was used for ChIP-seq. See Zhang et al., 2012 for details.
      Biological Information
      ImmunogenRecombinant protein corresponding to Human Mi-2β (CHD-4).
      Clone17H11
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityRecognizes Mi-2β (CHD-4)
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CHD4
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~ 220 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in a Jurkat cell lystate.

      Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Mi-2β (CHD-4) in a Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Mi-2β, clone 17H11 - Q2469322 Q2469322

      References

      Reference overviewPub Med ID
      Harnessing of the nucleosome-remodeling-deacetylase complex controls lymphocyte development and prevents leukemogenesis.
      Zhang, J; Jackson, AF; Naito, T; Dose, M; Seavitt, J; Liu, F; Heller, EJ; Kashiwagi, M; Yoshida, T; Gounari, F; Petrie, HT; Georgopoulos, K
      Nature immunology 13 86-94 2012

      Show Abstract
      22080921 22080921

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies