A part of MilliporeSigma

AB5675 | Anti-Metabotropic Glutamate Receptor 5 Antibody, pain

AB5675
50 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R IHC, WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB5675
      ReplacesAB1596; 06-451
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Metabotropic Glutamate Receptor 5 Antibody, pain
      Alternate Names
      • Metabotropic glutamate receptor 5
      • Metabotropic Glutamate Receptor 5 Antibody
      • mGluR5
      • Anti-mGluR5
      Background InformationMetabotropic glutamate receptor 5 or mGluR5 (UniProt Q3UVX5; also known as G protein-coupled receptor GRM5, G protein coupled receptor family C, group 1, member E) is encoded by the Grm5 (also known as Gprc1e, Mglur5, AI850523, 6430542K11Rik) gene in murine species (Gene ID 108071). The metabotropic glutamate receptors belong to a family of G protein-coupled receptors that have been divided into 3 groups based on their sequence homology, putative signal transduction mechanisms, and pharmacologic properties. mGluR1 and mGluR5 constitute Group I metabotropic glutamate receptors. mGluR5 is found on postsynaptic terminals of neurons and microglia. mGluR5 signals via Gq/G11 coupling to activate phospholipase C, resulting in calcium mobilization and activation of protein kinase C (PKC). In addition, mGluR5 is also reported to mediate signaling involving Src-family protein tyrosine kinase in a G protein-independent manner.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Brain tissue
      PresentationPurified rabbit polyclonal antibody in buffer containing PBS with 0.05% sodium azide.
      Applications
      ApplicationAnti-Metabotropic Glutamate Receptor 5 Antibody, pain is an antibody against Metabotropic Glutamate Receptor 5 for use in IH & WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern Blotting Analysis: 0.1-0.5 μg/mL using ECL on rat brain microsomal preparation. Reacts with a band of ~132 kDa.
      Immunohistochemistry: 0.1-0.2 μg/mL on rat brain and mouse brain sections fixed with 4% paraformaldehyde containing 15% saturated picric acid in 0.1M PB, pH 7.4.
      Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Metabotropic Glutamate Receptor 5 in mouse hindbrain and rat colon tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the cytoplasmic domain of mouse Metabotropic Glutamate Receptor 5.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody will not detect Isoform 2.
      Species Reactivity
      • Mouse
      • Rat
      Species Reactivity NoteMouse, Rat. Predicted to react with Human, Bovine, Primate, Feline, Horse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryL-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Alternative splice variants of GRM8 have been described but their full-length nature has not been determined.
      Gene Symbol
      • GRM5
      • mGluR5
      • MGLUR5A
      • mGlu5
      • MGLUR5
      • MGLUR5B
      • GPRC1E
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P41594 # Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol- calcium second messenger system and generates a calcium-activated chloride current.
      SIZE: 1212 amino acids; 132469 Da
      SUBUNIT: The PPXXF motif binds HOM1, HOM2 and HOM3. Interacts with SIAH1, RYR1, RYR2, ITPR1, SHANK1, SHANK3 and GRASP (By similarity).
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
      SIMILARITY: SwissProt: P41594 ## Belongs to the G-protein coupled receptor 3 family.
      Molecular Weight~150 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in microsomal preparation of rat brain tissue lysate.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected Metabotropic Glutamate Receptor 5 in 10 µg of microsomal preparation of rat brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Metabotropic Glutamate -2585810 2585810
      Anti-Metabotropic Glutamate -2625897 2625897
      Anti-Metabotropic Glutamate -2757164 2757164
      Anti-Metabotropic Glutamate -2789806 2789806
      Anti-Metabotropic Glutamate Receptor 5 -2710232 2710232
      RABBIT ANTI- METABOTROPIC GLUTAMATE RECEPTOR 5 2455643
      RABBIT ANTI- METABOTROPIC GLUTAMATE RECEPTOR 5 (mGluR5) AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2373390 2373390
      RABBIT ANTI- METABOTROPIC GLUTAMATE RECEPTOR 5 (mGluR5) AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2463663 2463663
      RABBIT ANTI- METABOTROPIC GLUTAMATE RECEPTOR 5 (mGluR5) AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2015979 2015979
      RABBIT ANTI- METABOTROPIC GLUTAMATE RECEPTOR 5 (mGluR5) AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2120961 2120961
      RABBIT ANTI- METABOTROPIC GLUTAMATE RECEPTOR 5 (mGluR5) - 2489067 2489067
      RABBIT ANTI- METABOTROPIC GLUTAMATE RECEPTOR 5 (mGluR5) AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2067768 2067768
      RABBIT ANTI- METABOTROPIC GLUTAMATE RECEPTOR 5 (mGluR5) AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2201444 2201444
      RABBIT ANTI- METABOTROPIC GLUTAMATE RECEPTOR 5 (mGluR5) AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2279534 2279534

      References | 19 Available | See All References

      Reference overviewApplicationPub Med ID
      Posttraumatic stress disorder-like induction elevates β-amyloid levels, which directly activates corticotropin-releasing factor neurons to exacerbate stress responses.
      Justice, NJ; Huang, L; Tian, JB; Cole, A; Pruski, M; Hunt, AJ; Flores, R; Zhu, MX; Arenkiel, BR; Zheng, H
      The Journal of neuroscience : the official journal of the Society for Neuroscience 35 2612-23 2015

      Show Abstract
      25673853 25673853
      Increased response to glutamate in small diameter dorsal root ganglion neurons after sciatic nerve injury.
      Gong, K; Kung, LH; Magni, G; Bhargava, A; Jasmin, L
      PloS one 9 e95491 2014

      Show Abstract
      Immunofluorescence24748330 24748330
      Therapeutic molecules and endogenous ligands regulate the interaction between brain cellular prion protein (PrPC) and metabotropic glutamate receptor 5 (mGluR5).
      Haas, LT; Kostylev, MA; Strittmatter, SM
      The Journal of biological chemistry 289 28460-77 2014

      Show Abstract
      25148681 25148681
      Time-dependent Gene Profiling Indicates the Presence of Different Phases for Ischemia/Reperfusion Injury in Retina.
      Andreeva, K; Zhang, M; Fan, W; Li, X; Chen, Y; Rebolledo-Mendez, JD; Cooper, NG
      Ophthalmology and eye diseases 6 43-54 2014

      Show Abstract
      25210480 25210480
      Synaptic depression via mGluR1 positive allosteric modulation suppresses cue-induced cocaine craving.
      Loweth, JA; Scheyer, AF; Milovanovic, M; LaCrosse, AL; Flores-Barrera, E; Werner, CT; Li, X; Ford, KA; Le, T; Olive, MF; Szumlinski, KK; Tseng, KY; Wolf, ME
      Nature neuroscience 17 73-80 2014

      Show Abstract
      24270186 24270186
      Hippocampal metaplasticity is required for the formation of temporal associative memories.
      Xu, J; Antion, MD; Nomura, T; Kraniotis, S; Zhu, Y; Contractor, A
      The Journal of neuroscience : the official journal of the Society for Neuroscience 34 16762-73 2014

      Show Abstract
      25505329 25505329
      SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties.
      Han, K; Holder, JL; Schaaf, CP; Lu, H; Chen, H; Kang, H; Tang, J; Wu, Z; Hao, S; Cheung, SW; Yu, P; Sun, H; Breman, AM; Patel, A; Lu, HC; Zoghbi, HY
      Nature 503 72-7 2013

      Show Abstract
      Western Blotting24153177 24153177
      Amyloid-β and Alzheimer's disease type pathology differentially affects the calcium signalling toolkit in astrocytes from different brain regions.
      Grolla, AA; Sim, JA; Lim, D; Rodriguez, JJ; Genazzani, AA; Verkhratsky, A
      Cell death & disease 4 e623 2013

      Show Abstract
      23661001 23661001
      MeCP2 phosphorylation is required for modulating synaptic scaling through mGluR5.
      Zhong, X; Li, H; Chang, Q
      The Journal of neuroscience : the official journal of the Society for Neuroscience 32 12841-7 2012

      Show Abstract
      22973007 22973007
      β-Estradiol unmasks metabotropic receptor-mediated metaplasticity of NMDA receptor transmission in the female rat dentate gyrus.
      Nebieridze, N; Zhang, XL; Chachua, T; Velíšek, L; Stanton, PK; Velíšková, J
      Psychoneuroendocrinology 37 1845-54 2012

      Show Abstract
      22541715 22541715

      Data Sheet

      Title
      RABBIT ANTI- METABOTROPIC GLUTAMATE RECEPTOR 5 (mGluR5) AFFINITY PURIFIED POLYCLONAL ANTIBODY

      Related Products & Applications

      Related Products

      Catalog Number Description  
      AB5675-AF488 Anti-Metabotropic Glutamate Receptor 5 Antibody, Alexa Fluor® 488 Show Pricing & Availability
      AB5675-AF555 Anti-Metabotropic Glutamate Receptor 5 Antibody, Alexa Fluor® 555 Show Pricing & Availability

      View Gene Related Products for this Target

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies