A part of MilliporeSigma

05-1049 | Anti-Met (extracellular) Antibody, clone 4F8.2

05-1049
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H IH(P), WB M Purified Monoclonal Antibody
      Description
      Catalogue Number05-1049
      Replaces05-237
      DescriptionAnti-Met (extracellular) Antibody, clone 4F8.2
      Alternate Names
      • HGF receptor
      • HGF/SF receptor
      • Met proto-oncogene tyrosine kinase
      • SF receptor
      • Scatter factor receptor
      • met proto-oncogene
      • met proto-oncogene (hepatocyte growth factor receptor)
      • oncogene MET
      Background InformationThe proto-oncogene Met (c-Met, HGF/SF Receptor, HGFR) is a receptor tyrosine kinase that is activated upon Hepatocyte Growth Factor (HGF) binding. This stimulates the autophosphorylation on Tyr1234/Tyr1235 which leads to pleiotropic downstream signaling events in epithelial cells that stimulate multiple cellular processes including cell proliferation, growth, differentiation, cell migration/ invasion, and tumorigenesis. These effects are mediated by SH2 domain-containing proteins that bind to the C-terminal tail of c-Met. Chronic stimulation of Met on cancer cells is thought to play a role in metastasis and dysregulation of c-Met activity is directly associated with oncogenesis, making it an important cancer target.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05% NaN3.
      Applications
      ApplicationDetect Met (extracellular) using this Anti-Met (extracellular) Antibody, clone 4F8.2 validated for use in IH(P) & WB.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesWestern Blot Analysis:
      This lot detected Met at 1:1,000 dilution in HEK293 cell lysate resolved via SDS-PAGE and transferred to PVDF.

      Immunohistochemistry Analysis :
      Biological Information
      ImmunogenGST-fusion protein corresponding to amino acids 80-434 of human Met.
      EpitopeExtracelluar domain (N-term)
      Clone4F8.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityDetects Met on its extracellular domain.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
      Gene Symbol
      • AUTS9
      • EC 2.7.10.1
      • HGFR
      • OTTHUMP00000069168
      • RCCP2
      • c-Met
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity.

      Catalytic activity ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

      SUBUNIT:Heterodimer formed of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked. Binds PLXNB1 and GRB2. Interacts with SPSB1, SPSB2 and SPSB4 By similarity. Interacts with INPP5D/SHIP1. When phosphorylated at Tyr-1356, interacts with INPPL1/SHIP2. Interacts with RANBP9 and RANBP10, as well as SPSB1, SPSB2, SPSB3 and SPSB4. SPSB1 binding occurs in the presence and in the absence of HGF, however HGF treatment has a positive effect on this interaction.

      LOCATION: Single-pass type I membrane protein.

      DOMAIN:The kinase domain is involved in SPSB1 binding.

      DISEASE: Activation of MET after rearrangement with the TPR gene produces an oncogenic protein.

      Defects in MET may be associated with gastric cancer.

      Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550].

      Defects in MET are a cause of hereditary papillary renal carcinoma (HPRC) [MIM:605074]; also known as papillary renal cell carcinoma 2 (RCCP2). HPRC is a form of inherited kidney cancer characterized by a predisposition to develop multiple, bilateral papillary renal tumors. The pattern of inheritance is consistent with autosomal dominant transmission with reduced penetrance.

      Genetic variations in MET may be associated with susceptibility to autism type 1B (AUTS1B) [MIM:608636]. Autism is a neurodevelopmental disorder characterized by disturbance in language, perception and socialization. The disorder is classically defined by a triad of limited or absent verbal communication, a lack of reciprocal social interaction or responsiveness, and restricted, stereotypical, and ritualized patterns of interests and behavior.

      SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family.

      Contains 3 IPT/TIG domains.

      Contains 1 protein kinase domain.

      Contains 1 Sema domain.

      Molecular Weight170 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot.

      Western Blot Analysis:
      This lot detected Met at 1:1,000 dilution in HEK293 cell lysate resolved via SDS-PAGE and transferred to PVDF.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Met (extracellular), clone 4F8.2 - 2128270 2128270
      Anti-Met (extracellular), clone 4F8.2 - 2433536 2433536
      Anti-Met (extracellular), clone 4F8.2 - 2297318 2297318
      Anti-Met (extracellular), clone 4F8.2 - DAM1581071 DAM1581071
      Anti-Met (extracellular), clone 4F8.2 - NG1551485 NG1551485
      Anti-Met (extracellular), clone 4F8.2 -2517865 2517865
      Anti-Met (extracellular), clone 4F8.2 -2763989 2763989

      References

      Reference overviewPub Med ID
      Dissecting the role of human embryonic stem cell-derived mesenchymal cells in human umbilical vein endothelial cell network stabilization in three-dimensional environments.
      Boyd, NL; Nunes, SS; Krishnan, L; Jokinen, JD; Ramakrishnan, VM; Bugg, AR; Hoying, JB
      Tissue engineering. Part A 19 211-23 2013

      Show Abstract
      22971005 22971005
      Cellular mechanisms underlying the regulation of dendritic development by hepatocyte growth factor.
      Charles Finsterwald,Jean-Luc Martin
      The European journal of neuroscience 34 2011

      Show Abstract
      21895802 21895802
      Crystal structure of the tyrosine kinase domain of the hepatocyte growth factor receptor c-Met and its complex with the microbial alkaloid K-252a.
      Schiering, Nikolaus, et al.
      Proc. Natl. Acad. Sci. U.S.A., 100: 12654-9 (2003) 2003

      14559966 14559966

      Brochure

      Title
      Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies