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MAB2635 | Anti-Mena Antibody, clone A351F7D9

100 µg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, H, M WB, IHC M Purified Monoclonal Antibody
      Catalogue NumberMAB2635
      DescriptionAnti-Mena Antibody, clone A351F7D9
      Alternate Names
      • enabled homolog (Drosophila)
      • Protein enabled homolog
      • Mammalian enabled
      Background InformationMammalian Enabled (Mena) is a protein belonging to ENA/VASP family, a group of proteins that are actin-associated and implicated in a variety cell processes. Specifically, Mena is involved in cell polarity, cell motility, cytoskeleton remodeling, filopodial, and lamellipodial dynamics within migrating cells. Three forms of Mena are expressed in embryonic head tissues and are identified based on molecular weight (140 kDa, 88 kDa, and 80 kDa). In adult wild-type tissues, Mena has been isolated in brain, testis, ovary, and fat in the 80 kDa form. Studies show the 140 kDa form is expressed only in adult brains while the 88 kDa form decreases to undetectable levels by E16, (Lanier, 1999). Mena contains 4 domains of interest; the Ena/VASP homologue 1 (EVH1) domain, an EVH2 domain, a proline-rich domain, and an Arg/Leu/Glu-risch region. Expression of Mena has been observed in the myoepithelia of breast, bronchial glands, parotid, and sweat glands. Mena has also been observed in the epithelium of mouse mucosal tissues: colon-rectum, endometrium, pancreas acinar ductal, placenta vascular media, and prostate fibromuscular stroma. Most breast cancer cell lines, along with lesions from primary breast tumor, demonstrate overexpression of Mena.
      Product Information
      • Rat hippocampus tissue lysate
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationDetect Mena using this Anti-Mena Antibody, clone A351F7D9 validated for use in WB, IH.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH, (Kwiatkowski, 2007).

      Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC, (Dent, 2007).
      Biological Information
      ImmunogenHistidine-tagged recombinant protein corresponding to human Mena and an additional fragment of Mena +, comprising only the + exon
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Specificity The antibody recognizes Mena and Mena alternate form (+ exon).
      Species Reactivity
      • Rat
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MENAH
      • ENAH
      • NDPP1
      • ENA
      Purification MethodProtein G
      UniProt Number
      UniProt SummaryFUNCTION: Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. Required for the actin-based mobility of Listeria monocytogenes (by similarity).

      SUBUNIT STRUCTURE: Homotetramer by similarity. Interacts with APBB1IP, APBB1, PFN1 and ROBO4. Isoforms, containing the polyproline-rich regions with PPLP motifs, bind the WW domain of APBB1IP. Isoforms, containing the PPSY motif, bind, in vitro, to the WW2 and WW3 domains of NEDD4 and to the WW1 domain of YAP1. Binds the SH3 domain of BAIAP2-alpha but only after the autoinhibitory region of BAIAP2-alpha has been blocked by interaction with CDC42. Interacts, via the EVH1/WH1 domain, with the Pro-rich domains from VCL, ZYX and Listeria monocytogenes actA and with the TES LIM domain. The TES LIM domain and the Pro-rich domains from VCL or ZYX compete for the same binding site. Interaction with ZYX is important for targeting ENAH to focal adhesions and enhances production of actin-rich structures at the apical surface of cells. Interacts, through the Pro-rich region, with the C-terminal SH3 domain of DNMPB. Binds GPHN (by similarity).

      SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm › cytoskeleton by similarity. Cell projection › lamellipodium By similarity. Cell projection › filopodium By similarity. Cell junction › synapse (by similarity). Cell junction › focal adhesion. Note: Targeted to the leading edge of lamellipodia and filopodia by MRL family members. Colocalizes at filopodial tips with a number of other proteins including vinculin and zyxlin. Colocalizes with N-WASP at the leading edge. Colocalizes with GPHN and PFN at synapses (by similarity).

      TISSUE SPECIFICTY: Expressed in myoepithelia of parotid, breast, bronchial glands and sweat glands. Expressed in colon-rectum muscolaris mucosae epithelium, pancreas acinar ductal epithelium, endometrium epithelium, prostate fibromuscolar stroma and placenta vascular media. Overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions.

      DOMAIN: The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.

      PTM: NTN1-induced PKA phosphorylation on Ser-265 directly parallels the formation of filopodial protrusions (by similarity).

      Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.13 Ref.14 Ref.15 Ref.16 Ref.17

      SEQUENCE SIMILARITIES: Belongs to the Ena/VASP family.

      Contains 1 WH1 domain.
      Molecular Weight ~ 80 kDa observed MW. In brain tissues, a 140 kDa MW band is also expected to be observed.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in rat hippocampus tissue lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected Mena in 10 µg of rat hippocampus tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Mena, clone A351F7D9 - 2192292 2192292
      Anti-Mena, clone A351F7D9 - NRG1777071 NRG1777071
      Anti-Mena, clone A351F7D9 -2500712 2500712
      Anti-Mena, clone A351F7D9 -2581269 2581269
      Anti-Mena, clone A351F7D9 -2608456 2608456
      Anti-Mena, clone A351F7D9 -2691335 2691335
      Anti-Mena, clone A351F7D9 -2705083 2705083


      Reference overviewApplicationPub Med ID
      Endophilin, Lamellipodin, and Mena cooperate to regulate F-actin-dependent EGF-receptor endocytosis.
      Vehlow, Anne, et al.
      EMBO J., (2013) 2013

      Show Abstract
      24076656 24076656
      SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties.
      Han, K; Holder, JL; Schaaf, CP; Lu, H; Chen, H; Kang, H; Tang, J; Wu, Z; Hao, S; Cheung, SW; Yu, P; Sun, H; Breman, AM; Patel, A; Lu, HC; Zoghbi, HY
      Nature 503 72-7 2013

      Show Abstract
      Western Blotting24153177 24153177

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