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ABE1928 | Anti-MafK/Nfe2u Antibody

ABE1928
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, H WB, ChIP, EMSA Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberABE1928
      DescriptionAnti-MafK/Nfe2u Antibody
      Alternate Names
      • Transcription factor MafK
      • MafK/Nfe2u
      • Erythroid transcription factor NF-E2 p18 subunit
      Background InformationTranscription factor MafK (UniProt Q61827; also known as Erythroid transcription factor NF-E2 p18 subunit, Nuclear factor erythroid derived 2 ubiquitous, p18 NF-E2) is encoded by the Mafk (also known as Nfe2u, AW061068) gene (Gene ID 17135) in murine species. MafK, MafG and MafF consistute a family of transcription factors that dimerize with the CNC (cap' n' collar) family regulatory protein Nrf2 (NF-E2 p45-related factor 2) via their bZip domain. The Nrf2/small Maf heterodimer regulates downstream genes, including those of phase II detoxifying enzymes, by targeting antioxidant responsive element (ARE) and Maf recognition element (MARE).
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal serum with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-MafK/Nfe2u Antibody is validated for use in Western Blotting, Chromatin Immunoprecipitation (ChIP) and Electrophoretic Mobility Shift Assay for the detection of MafK/Nfe2u.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      • Electrophoretic Mobility Shift Assay
      Application NotesWestern Blotting Analysis: A representative lot detected endogenous MafK as well as Zn+2-induced expression of exogenous MafK using nuclear extracts from a mouse erythroleukemia (MEL) cell line transfected with MafK cDNA in a metallothionein IIA gene promoter-driven expression plasmid (Igarashi, K., et al. (1995). Proc Natl Acad Sci U S A. 92(16):7445-7449).
      Chromatin Immunoprecipitation Analysis: A representative lot detected MafK association with the GST-P enhancer (GPE1) in rat hepatoma cells (H4IIE and dRLh84) and hyperplastic nodules/HN-bearing pre-neoplastic rat hepatocytes, but not in normal rat hepatocytes. While MafK association with the NAD(P)H:quinone oxidoreductase 1 (NQO1) antioxidant responsive element (ARE) was detected in all four sample types (Ikeda, H., et al. (2004). Biochem J. 380(Pt 2):515-521).
      Electrophoretic Mobility Shift Assay Analysis: A representative lot caused a supershift of MafK-containing complex in EMSA using radiolabeled oligonucleotides containing PBGD gene NF-E2 binding site and nuclear extracts from a mouse erythroleukemia (MEL) cell line induced to overexpress MafK (Igarashi, K., et al. (1995). Proc Natl Acad Sci U S A. 92(16):7445-7449).
      Electrophoretic Mobility Shift Assay (EMSA): A representative lot caused a supershift of both the constitutive and tBHQ-induced ARE-binding complex (Complex I and II, respectively) in EMSA using a radiolabeled oligonucleotide containing antioxidant responsive element (ARE) NF-E2 binding site and nuclear extracts from untreated and tBHQ-treated K562 cells (Kim, Y.C., et al. (2003). Oncogene. 22(12):1860-1865).
      Biological Information
      ImmunogenMaltose binding protein-tagged recombinant protein corresponding to mouse MafK/Nfe2u.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Mouse
      • Rat
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Mafk
      • Nfe2u
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~18 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MEL (mouse erythroleukemia) cell lysate.

      Western Blotting Analysis: 1:2,000 dilution of this antibody detected MafK/NF-E2 in 20 µg of MEL (mouse erythroleukemia) cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-MafK/Nfe2u -Q2608325 Q2608325

      References

      Reference overviewPub Med ID
      Transcription factor Nrf2/MafK regulates rat placental glutathione S-transferase gene during hepatocarcinogenesis.
      Ikeda, H; Nishi, S; Sakai, M
      The Biochemical journal 380 515-21 2004

      Show Abstract
      14960151 14960151
      Thioredoxin-dependent redox regulation of the antioxidant responsive element (ARE) in electrophile response.
      Kim, YC; Yamaguchi, Y; Kondo, N; Masutani, H; Yodoi, J
      Oncogene 22 1860-5 2003

      Show Abstract
      12660821 12660821
      Conditional expression of the ubiquitous transcription factor MafK induces erythroleukemia cell differentiation.
      Igarashi, K; Itoh, K; Hayashi, N; Nishizawa, M; Yamamoto, M
      Proceedings of the National Academy of Sciences of the United States of America 92 7445-9 1995

      Show Abstract
      7638211 7638211

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