A part of MilliporeSigma

MABT495 | Anti-MYL3, clone 7C1 Antibody

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R WB, IHC M Ascites Monoclonal Antibody
      Catalogue NumberMABT495
      DescriptionAnti-MYL3, clone 7C1 Antibody
      Alternate Names
      • Myosin light chain 3
      • Cardiac myosin light chain 1
      • CMLC1
      • Myosin light chain 1
      • slow-twitch muscle B/ventricular isoform
      • MLC1SB
      • Ventricular/slow twitch myosin alkali light chain
      Background InformationMyosin light chain 3 (MYL3), also known as Cardiac myosin light chain 1 (CMLC1) or Myosin light chain 1 slow-twitch muscle B/ventricular isoform (MLC1SB) or Ventricular/slow twitch myosin alkali light chain (VLC1) functions as a regulatory light chain of myosin. Myosins are a large superfamily of motor proteins that move along actin filaments, while hydrolyzing ATP. Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains and four light chains. Activation of smooth and cardiac muscle primarily involves pathways which increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Human myosin light chain has clinical application as a cardiac marker. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy.
      Product Information
      PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
      ApplicationDetect MYL3 using this Anti-MYL3, clone 7C1 Antibody validated for use in western blotting & IHC.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected MYL3 in human skeletal muscle and cardiac muscle tissues.
      Biological Information
      ImmunogenPurified recombinant fragment of human MYL3 expressed in E. Coli
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Rat
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • MYL3
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~25 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat fetal heart tissue lysate.

      Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected MYL3 in rat fetal heart tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information