IM09L Anti-MMP-9 (Ab-1) Mouse mAb (6-6B)

IM09L
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      IM09L-100UG
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          Plastic ampoule 100 μg
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          IM09L-1MG
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              Plastic ampoule 1 mg
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              Description
              OverviewRecognizes the ~92 kDa latent and the ~83 kDa active forms of MMP-9 under non-reducing conditions, but only the latent form under reducing conditions. Inhibits the enzymatic activity of MMP-9. For paraffin sections, use Cat. No. IM37L.
              Catalogue NumberIM09L
              Brand Family Calbiochem®
              SynonymsAnti-Type IV Collagenase, Anti-Gelatinase B, Anti-92 kDa Gelatinase, Anti-Matrix Metalloproteinase 9
              References
              ReferencesCottam, D.W. and Rees, R.C. 1993. Intl. J. of Oncol. 2, 861.
              Nakajima, M., et al. 1993. Cancer Res. 53, 5802.
              Ramos-DeSimone, et al. 1993. Hybridoma. 12, 349.
              Stetler-Stevenson, W.G., et al. 1993. FASEB. 7, 1434.
              Zucker, S., et al. 1993. Cancer Res. 53, 140.
              Woessner, J.F. 1991. FASEB. 5, 2145.
              Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1; 99-106.
              Product Information
              DeclarationNot available for sale in Japan.
              FormLyophilized
              FormulationLyophilized from a volatile buffer, 100 µg BSA.
              Positive controlMMP-9 protein (Cat. Nos. PF024 or PF038)
              PreservativeNone
              Applications
              Application ReferencesImmunoprecipitation Ramos-DeSimone, N. and French, D. L. 1994. Ann. NY Acad. Sci. 732, 469. Immunoblotting, Neutralization Studies Ramos-DeSimone, N., et al. 1993. Hybridoma. 12, 349.
              Key Applications Neutralization Studies
              Immunoblotting (Western Blotting)
              Immunoprecipitation
              Not Paraffin Sections
              Application NotesImmunoblotting (2 µg/ml)
              Immunoprecipitation (see appplication references)
              Neutralization Studies (see comments and application references)
              Paraffin Sections (not recommended)
              Application CommentsInhibits MMP-9 enzymatic activity. For staining paraffin sections, use Anti-MMP-9 (626-644) (Ab-3) Mouse mAb (56-2A4) (Cat. No. IM37L). Antibody should be titrated for optimal results in individual systems.
              Biological Information
              ImmunogenMMP-9 from conditioned medium of PMA-stimulated HT-1080 human fibrosarcoma cells
              ImmunogenHuman
              Clone6-6B
              HostMouse
              IsotypeIgG₁
              Species Reactivity
              • Human
              Antibody TypeMonoclonal Antibody
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Standard Handling
              Storage +2°C to +8°C
              Do not freeze Ok to freeze
              Special InstructionsStore at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Resuspend the antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Freezing of aliquots is best for long-term storage of reconstituted product; repetitive freezing and thawing should be avoided.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              Anti-MMP-9 (Ab-1) Mouse mAb (6-6B) SDS

              Title

              Safety Data Sheet (SDS) 

              Anti-MMP-9 (Ab-1) Mouse mAb (6-6B) Certificates of Analysis

              TitleLot Number
              IM09L

              References

              Reference overview
              Cottam, D.W. and Rees, R.C. 1993. Intl. J. of Oncol. 2, 861.
              Nakajima, M., et al. 1993. Cancer Res. 53, 5802.
              Ramos-DeSimone, et al. 1993. Hybridoma. 12, 349.
              Stetler-Stevenson, W.G., et al. 1993. FASEB. 7, 1434.
              Zucker, S., et al. 1993. Cancer Res. 53, 140.
              Woessner, J.F. 1991. FASEB. 5, 2145.
              Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1; 99-106.
              Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision27-August-2007 RFH
              SynonymsAnti-Type IV Collagenase, Anti-Gelatinase B, Anti-92 kDa Gelatinase, Anti-Matrix Metalloproteinase 9
              ApplicationImmunoblotting (2 µg/ml)
              Immunoprecipitation (see appplication references)
              Neutralization Studies (see comments and application references)
              Paraffin Sections (not recommended)
              DescriptionPurified mouse monoclonal antibody. Recognizes the ~92 kDa latent and the ~83 kDa active forms of human MMP-9 under non-reducing conditions, but only the latent form under reducing conditions.
              BackgroundMatrix metalloproteinases (MMP's) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMP's share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an ~10 kDa segment from the N terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis. MMP-9 (Gelatinase B, 92 kDa gelatinase/type IV collagenase) is secreted as a 92 kDa zymogen which is proteolytically processed to the 83 kDa active form; a 68 kDa has also been detected. MMP-9, along with its most closely related member of the MMP family, MMP-2, show substrate specificity toward type IV and V collagens, gelatin and elastin. Numerous studies have shown a correlation between collagenase expression and metastatic potential. Elevated levels of MMP-9 in plasma suggest that it may be a useful marker for the diagnosis or prognosis of cancer in general.
              HostMouse
              Immunogen speciesHuman
              ImmunogenMMP-9 from conditioned medium of PMA-stimulated HT-1080 human fibrosarcoma cells
              Clone6-6B
              IsotypeIgG₁
              Specieshuman
              Positive controlMMP-9 protein (Cat. Nos. PF024 or PF038)
              FormLyophilized
              FormulationLyophilized from a volatile buffer, 100 µg BSA.
              PreservativeNone
              CommentsInhibits MMP-9 enzymatic activity. For staining paraffin sections, use Anti-MMP-9 (626-644) (Ab-3) Mouse mAb (56-2A4) (Cat. No. IM37L). Antibody should be titrated for optimal results in individual systems.
              Storage +2°C to +8°C
              Do Not Freeze Ok to freeze
              Special InstructionsStore at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Resuspend the antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Freezing of aliquots is best for long-term storage of reconstituted product; repetitive freezing and thawing should be avoided.
              Toxicity Standard Handling
              ReferencesCottam, D.W. and Rees, R.C. 1993. Intl. J. of Oncol. 2, 861.
              Nakajima, M., et al. 1993. Cancer Res. 53, 5802.
              Ramos-DeSimone, et al. 1993. Hybridoma. 12, 349.
              Stetler-Stevenson, W.G., et al. 1993. FASEB. 7, 1434.
              Zucker, S., et al. 1993. Cancer Res. 53, 140.
              Woessner, J.F. 1991. FASEB. 5, 2145.
              Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1; 99-106.
              Application referencesImmunoprecipitation Ramos-DeSimone, N. and French, D. L. 1994. Ann. NY Acad. Sci. 732, 469. Immunoblotting, Neutralization Studies Ramos-DeSimone, N., et al. 1993. Hybridoma. 12, 349.

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              Categories

              Life Science Research > Antibodies and Assays > Primary Antibodies