IM71 Anti-MMP-7 (Ab-3) Mouse mAb (ID2)

IM71
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      IM71-100UG
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
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      Available
        Remaining : Will advise
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          Will advise
          Contact Customer Service
          Contact Customer Service

          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the ~28 kDa latent and the ~19 kDa active forms of MMP-7 in SW620 cells and bladder, breast, and ovarian carcinoma tissue.
          Catalogue NumberIM71
          Brand Family Calbiochem®
          SynonymsAnti-PUMP-1, Anti-Matrilysin, Anti-Matrix Metalloproteinase 7
          References
          ReferencesBrunner, K.L, et al. 1995. Proc. Natl. Acad. Sci. USA 92, 7362.
          Imai, K., et al. 1995. J. Biol. Chem. 270, 6691.
          Lichtinghagen, R., et al. 1995. Eur. J. Clin. Chem. Clin. Biochem. 33, 65.
          Nakano, A., et al. 1995. J. Neurosurg. 83, 298.
          Woessner, J.F., Jr., 1995. Meth. Enzymol. 248, 485.
          Yamamoto, H., et al. 1995. J. Clin. Lab. Anal. 9, 297.
          Gaire, M., et al. 1994. J. Biol. Chem. 269, 2032.
          McDonnell, S., et al. 1994. Biochem. Soc. Trans. 22, 58.
          Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
          Stetler-Stevenson, W.G. 1993. FASEB J. 7, 1434.
          Woessner, J.F. 1991. FASEB J. 5, 2145. Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99.
          Sellers, A. and Woessner, J.F., Jr. 1980. Biochem. J. 189, 521.
          Product Information
          DeclarationNot available for sale in Japan.
          FormLiquid
          FormulationIn 10 mM PBS, pH 7.4.
          Positive controlConditioned, serum-free medium from SW620 cells or bladder, breast, or ovarian carcinoma
          PreservativeNone
          Applications
          Application ReferencesImmunoblotting Windsor, L.J., et al. 1997. J. Biochim. Biophys. Acta 1334, 261.
          Key Applications Paraffin Sections
          Affinity Purification
          Immunoblotting (Western Blotting)
          Immunofluorescence
          Application NotesAffinity Purification (see comments)
          Immunoblotting (see application references)
          Immunofluorescence (see comments)
          Paraffin Sections (4-8 µg/ml, no pre-treatment required)
          Application CommentsThis antibody has also been reported to work for immunofluorescence and affinity purification methods. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenrecombinant human MMP-7
          ImmunogenHuman
          CloneID2
          HostMouse
          IsotypeIgG2b
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-MMP-7 (Ab-3) Mouse mAb (ID2) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-MMP-7 (Ab-3) Mouse mAb (ID2) Certificates of Analysis

          TitleLot Number
          IM71

          References

          Reference overview
          Brunner, K.L, et al. 1995. Proc. Natl. Acad. Sci. USA 92, 7362.
          Imai, K., et al. 1995. J. Biol. Chem. 270, 6691.
          Lichtinghagen, R., et al. 1995. Eur. J. Clin. Chem. Clin. Biochem. 33, 65.
          Nakano, A., et al. 1995. J. Neurosurg. 83, 298.
          Woessner, J.F., Jr., 1995. Meth. Enzymol. 248, 485.
          Yamamoto, H., et al. 1995. J. Clin. Lab. Anal. 9, 297.
          Gaire, M., et al. 1994. J. Biol. Chem. 269, 2032.
          McDonnell, S., et al. 1994. Biochem. Soc. Trans. 22, 58.
          Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
          Stetler-Stevenson, W.G. 1993. FASEB J. 7, 1434.
          Woessner, J.F. 1991. FASEB J. 5, 2145. Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99.
          Sellers, A. and Woessner, J.F., Jr. 1980. Biochem. J. 189, 521.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-March-2009 JSW
          SynonymsAnti-PUMP-1, Anti-Matrilysin, Anti-Matrix Metalloproteinase 7
          ApplicationAffinity Purification (see comments)
          Immunoblotting (see application references)
          Immunofluorescence (see comments)
          Paraffin Sections (4-8 µg/ml, no pre-treatment required)
          DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with p3-X63-Ag8.653 mouse myeloma cells. Recognizes both the ~28 kDa latent and the ~18 kDa active forms of MMP-7.
          BackgroundMatrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an ~10 kDa segment from the N-terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in angiogenesis, arthritis, periodontitis, and metastasis. Matrix metalloproteinase-7 (MMP-7) also known as matrilysin and PUMP (EC 3.4.24.23) cleaves a number of substrates including collagen types IV and X, elastin, fibronectin, gelatin, laminin and proteoglycans. MMP-7 is closely related to the stromelysin family members but is encoded by a different gene. MMP-7 is the smallest of all the MMPs consisting of a pro-peptide domain and a catalytic domain. It lacks the hemopexin-like domain common to other members of the MMPs. MMP-7 is secreted as a 28 kDa proenzyme and can be activated in vitro by organomercurials (e.g., 4-aminophenylmercuric acetate, APMA) and trypsin and in vivo by MMP-3 to a 18 kDa active MMP-7 enzyme. Once activated, MMP-7 can activate pro-MMP-1 and pro-MMP-9 but not pro-MMP-2. MMP-7 is widely expressed having been reported in elevated levels in cycling endometrium as well as in colorectal cancers and adenomas, hepatocellular carcinomas, rectal carcinomas, and ~50% of gliomas.
          HostMouse
          Immunogen speciesHuman
          Immunogenrecombinant human MMP-7
          CloneID2
          IsotypeIgG2b
          Specieshuman
          Positive controlConditioned, serum-free medium from SW620 cells or bladder, breast, or ovarian carcinoma
          FormLiquid
          FormulationIn 10 mM PBS, pH 7.4.
          Concentration Label Please refer to vial label for lot-specific concentration
          PreservativeNone
          CommentsThis antibody has also been reported to work for immunofluorescence and affinity purification methods. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesBrunner, K.L, et al. 1995. Proc. Natl. Acad. Sci. USA 92, 7362.
          Imai, K., et al. 1995. J. Biol. Chem. 270, 6691.
          Lichtinghagen, R., et al. 1995. Eur. J. Clin. Chem. Clin. Biochem. 33, 65.
          Nakano, A., et al. 1995. J. Neurosurg. 83, 298.
          Woessner, J.F., Jr., 1995. Meth. Enzymol. 248, 485.
          Yamamoto, H., et al. 1995. J. Clin. Lab. Anal. 9, 297.
          Gaire, M., et al. 1994. J. Biol. Chem. 269, 2032.
          McDonnell, S., et al. 1994. Biochem. Soc. Trans. 22, 58.
          Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
          Stetler-Stevenson, W.G. 1993. FASEB J. 7, 1434.
          Woessner, J.F. 1991. FASEB J. 5, 2145. Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99.
          Sellers, A. and Woessner, J.F., Jr. 1980. Biochem. J. 189, 521.
          Application referencesImmunoblotting Windsor, L.J., et al. 1997. J. Biochim. Biophys. Acta 1334, 261.