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MABF1413 | Anti-MHC class II (I-A/I-E), violetFluor™ 450, clone M5/114 .15.2 Antibody

MABF1413
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M FC R violetFluor™ 450 Monoclonal Antibody
      Description
      Catalogue NumberMABF1413
      DescriptionAnti-MHC class II (I-A/I-E), violetFluor™ 450, clone M5/114 .15.2 Antibody
      Alternate Names
      • H-2 class II histocompatibility antigen gamma chain
      • Ia antigen-associated invariant chain (Ii)
      • MHC class II-associated invariant chain
      • CD74
      • MHC class II (I-A/I-E)
      Background InformationThe M5/114.15.2 antibody reacts with mouse MHC Class II alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, as well as being cross-reactive with mouse cells of H-2p and H-2r haplotype. MHC Class II is widely expressed by mouse immune cells bearing these alloantigens, including T and B cells, monocytes, macrophages, and dendritic cells. The antibody does not react with the following alloantigens: I-Af, I-Ak, I-As, or NOD H-2g7The M5/114.15.2 antibody may be used for analysis of mouse cells expressing MHC Class II alloantigens as described. Please note that the M5/114.15.2 clone may also be referred to as M5/114 in the literature.
      References
      Product Information
      FormatvioletFluor™ 450
      PresentationPurified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
      Applications
      ApplicationThis rat monoclonal Anti-MHC class II (I-A/I-E) Antibody, violetFluor™ 450, clone M5/114 .15.2, Cat. No. MABF1413 is validated for use in Flow Cytometry, for the detection of MHC class II (I-A/I-E).
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenImmunogen information for this antibody is proprietary information.
      CloneM5/114.15.2
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      IsotypeIgG2bκ
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • CD74
      • Ii
      Purification MethodPurified by size-exclusion chromatography
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceThis monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

      Flow Cytometry Analysis: 0.25 μg from a representative lot detected MHC class II (I-A/I-E) in one million C57Bl/6 splenocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies