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MAB16200-AF488 | Anti-MGMT Antibody, clone MT 3.1, Alexa Fluor® 488 Conjugate

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H FC, ICC M AlexaFluor®488 Monoclonal Antibody
      Catalogue NumberMAB16200-AF488
      DescriptionAnti-MGMT Antibody, clone MT 3.1, Alexa Fluor® 488 Conjugate
      Alternate Names
      • Methylated-DNA-protein-cysteine methyltransferase
      • 6-O-methylguanine-DNA methyltransferase
      • GATase
      • MGMT
      • O-6-methylguanine-DNA-alkyltransferase
      Background InformationMethylated-DNA-protein-cysteine methyltransferase (EC; UniProt P16455; also known as 6-O-methylguanine-DNA methyltransferase, GATase, MGMT, O-6-methylguanine-DNA-alkyltransferase) is encoded by the MGMT gene (Gene ID 4255) in human. MGMT is a DNA repair enzyme that removes alkyl groups from O6-guanine and plays a fundamental role in maintaining genomic integrity. MGMT converts the naturally occurring mutagenic DNA lesion O6-methylguanine back to guanine, thereby prevents mismatch and errors during DNA replication and transcription. MGMT expression level correlates with cellular resistance to alkylnitrosoureas, compounds that induce O6-alkylguanine lesions. MGMT transcription is negatively regulated via promoter methylation, MGMT promoter methylation level is reported to correclate with the survival rate of glioblastoma patients receiving treatment with alkylating agents.
      Product Information
      PresentationPurified mouse IgG1 Alexa Fluor® 488 conjugate in PBS with 1.5% BSA and 0.05% sodium azide.
      ApplicationAnti-MGMT Antibody, clone MT 3.1, Alexa Fluor® 488 Conjugate, Cat. No. MAB16200-AF488, is a highly specific mouse monoclonal antibody that targets MGMT and has been tested in Flow Cytometry and Immunocytochemistry.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      Application NotesFlow Cytometry Analysis: 0.2 µL from a representative lot detected MGMT in one million MCF-7 cells.

      This monoclonal antibody is also available in unconjugated and Alexa Fluor® 647-conjugated forms (Cat. No. MAB16200 and MAB16200-AF647) for flow cytometry, immunocytochemistry, immunohistochemistry, immunoprecipitation, and Western blotting applications.
      Biological Information
      ImmunogenGATase purified from CEM-CCRF human leukemic lymphoblasts (Harris, L.C., et al. (1996). Cancer Res. 56(9):2029-2032; Brent, T.P., et al. (1990). Cancer Res. 50(1):58-61).
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityClone MT 3.1 detected p53 expression-dependent MGMT downregulation in IMR90 cells (Harris, L.C., et al. (1996). Cancer Res. 56(9):2029-2032).
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MGMT
      Purification MethodProtein A purified.
      UniProt Number
      Molecular Weight21.65 kDa calculated. ~25 kDa reported (Brent, T.P., et al. (1990). Cancer Res. 50(1):58-61).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in MCF-7 cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this Alexa Fluor® 488-conjugated antibody detected MGMT in MCF-7 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information