A part of MilliporeSigma

MABS529 | Anti-MEKK5 Antibody, clone TC003

100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers


      Contact Customer Service

      Click To Print This Page


      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M WB R Purified Monoclonal Antibody
      Catalogue NumberMABS529
      DescriptionAnti-MEKK5 Antibody, clone TC003
      Alternate Names
      • ASK1
      • Mitogen-activated protein kinase kinase kinase 5
      • Apoptosis signal-regulating kinase 1
      • ASK-1
      • MAPK/ERK kinase kinase 5
      • MEK kinase 5
      • MEKK 5
      Background InformationMEKK5 (MAP3K5) is a key kinase for our innate immunity and for mediating cellular signaling cascades evoked by changes in the external cellular environment. As such, MEKK5 is critical for cell fate during development, apoptosis, and cell survival as well as in modulating the host defense responses from a wide range of pathogens. MEKK5 acts as an upstream activator of the MKK/JNK signaling pathways and the p38 MAPK signaling pathways that ultimately results in activating multiple kinases such as MAP2K3/Mkk3 or MKK6/MMK7. MEKK5 is inactive as an unphosphorylated homodimer, and MEKK5 binds both upstream activators and downstream substrates in multi-molecular complexes. Expression is widespread and localized in the cytoplasmic in the perinuclear endoplasmic reticulum region, and MEKK5 is abundantly expressed in heart and pancreas. MEKK5 is regulated via phosphorylation and ubiquitination and degradation via the proteasome.
      Product Information
      PresentationPurified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-MEKK5 Antibody, clone TC003 is an antibody against MEKK5 for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected MEKK5 in 10 µg of bone marrow-derived macrophages tissue lysate (Dr. Maruyama, J. and Noguchi, T., Cell Signaling, Graduate School of Pharmaceutical Sciences, the University of Tokyo).
      Biological Information
      ImmunogenRecombinant protein corresponding to the C-terminus of mouse MEKK5.
      ConcentrationPlease refer to lot specific datasheet.
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Map3k5
      • Ask1
      • Mekk5
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~155 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in RAW 264.7 cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected MEKK5 in 10 µg of RAW 264.7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information