A part of MilliporeSigma

ABC155 | Anti-MDC1 Antibody

100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers


      Contact Customer Service

      Click To Print This Page


      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, ICC, IP Rb Affinity Purified Polyclonal Antibody
      Catalogue NumberABC155
      DescriptionAnti-MDC1 Antibody
      Alternate Names
      • Mediator of DNA damage checkpoint protein 1
      • Nuclear factor with BRCT domains 1
      Background InformationMediator of DNA damage checkpoint protein 1 (MDC1) is a subunit of the cohesin complex and is an essential protein required for sister chromatid cohesion in mitosis and meiosis. Temperature-sensitive mutations of this protein have been associated with defects in sister chromatid cohesion, chromosome condensation, and chromosome segregation. Its expression is cell cycle regulated and peaks in S phase. Expression of MDC1 is required for the S- and G2/M-checkpoint-mediated cell cycle arrest in response to DNA damage. MDC1 has been shown to interact with H2A.X near sites of DNA double-strand breaks and recruit ATM kinase to the damaged region of DNA. Consistent with this role, MDC1 also appears to modulate the function of other proteins involved in the DNA damage checkpoint such as BRCA1 and Chk2.
      Product Information
      FormatAffinity Purified
      • HeLa cells
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-MDC1 Antibody is a highly specific rabbit polyclonal antibody, that targets MDC1 (Mediator of DNA damage checkpoint protein 1) & has been tested in western blotting, ICC & IP.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: 10 µg from a representative lot detected MDC1 in 0.5 mg of HEK293 nuclear extract.

      Western Blotting Analysis: 2 µg/mL from a representative lot detected MDC1 in 10 µg of HEK293 nuclear extract.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the human MDC1.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Chimpanzee based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MDC1
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~184 kDa observed. Four isoforms are known to exist at ~227 kDa, ~198 kDa, ~196 kDa, and ~117 kDa due to alternative splicing. This protein has been observed at ~184 kDa in some cell lysates (See Cat. No. 05-1572). Bands below ~184 kDa may be observed due to degradation as part of the mechanism of action of MDC1 (Solier, S., and Pommier, Y. (2010). Cancer Res. 71(3):906-913.). Uncharacterized bands may be observed in some cell lysates.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected MDC1 in 10 µg of HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information