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04-1147 | Anti-MCAM [CD146] Antibody, clone EPR3208, rabbit monoclonal

100 µL  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB, FC, IP, IH(P), ICC Rb Culture Supernatant Monoclonal Antibody
      Catalogue Number04-1147
      DescriptionAnti-MCAM [CD146] Antibody, clone EPR3208, rabbit monoclonal
      Alternate Names
      • CD146 antigen
      • Cell surface glycoprotein P1H12
      • Melanoma-associated antigen A32
      • Melanoma-associated antigen MUC18
      • S-endo 1 endothelial-associated antigen
      • melanoma adhesion molecule
      • melanoma cell adhesion molecule
      Background InformationMCAM (MUC18 antigen, CD146), a member of the immuoglobulin superfamily, is an intrinsic membrane glycoprotein of 110-120 kDa found on the surface of endothelial cells, bone marrow fibroblasts and various melanomas. MCAM (Melanoma adhesion molecule) has been used as a marker of tumor progression in human melanoma because expression in those tumors correlates strongly with poor prognosis and the development of metastic disease. In addition, a number of human T, B and myeloid leukemic cell lines seem to express MCAM. The close structural relationship with N-CAM and related molecules suggests that MCAM may be also a developmentally regulated cell adhesion.
      Product Information
      FormatCulture Supernatant
      • A375 cells, fetal artery, and HUVEC lysates.
      PresentationCell culture supernatant containing Rabbit Monoclonal antibody in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      ApplicationThis Anti-MCAM [CD146] Antibody, clone EPR3208, Rabbit is validated for use in WB, FC, IP, IH(P), IC for the detection of MCAM [CD146].
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Immunoprecipitation
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      Application NotesImmunohistochemistry Analysis: 1:250 - 500 dilution from a previous lot detected MCAM in melanoma tissue.
      Immunocytochemistry Analysis: 1:100-250 dilution from a previous lot was used on IC.
      Immunoprecipitation: 1:10 dilution from a previous lot was used on IP.
      Flow Cytometry: 1:80 dilution from a previous lot was used on FC.
      Biological Information
      ImmunogenSynthetic peptide corresponding to residues in human MCAM.
      SpecificityThis antibody recognizes MCAM.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CD146
      • Gicerin
      • MUC18
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.
      SEQUENCE SIMILARITIES: Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 2 Ig-like V-type (immunoglobulin-like) domains.
      Molecular Weight~ 113 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on A375 cell lysate, fetal artery, and HUVEC lysate.

      Western Blot Analysis: 1:10,000 dilution of this antibody was used to detect MCAM in A375 cell lysate, fetal artery, and HUVEC lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.

      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-MCAM [CD146], clone EPR3208 Rabbit Monoclonal - 2140064 2140064
      Anti-MCAM [CD146], clone EPR3208 Rabbit Monoclonal - 2360001 2360001
      Anti-MCAM [CD146], clone EPR3208 Rabbit Monoclonal - 2384757 2384757
      Anti-MCAM [CD146], clone EPR3208 Rabbit Monoclonal - 2390381 2390381
      Anti-MCAM [CD146], clone EPR3208 Rabbit Monoclonal - 2424646 2424646
      Anti-MCAM [CD146], clone EPR3208 Rabbit Monoclonal - 2436298 2436298
      Anti-MCAM [CD146], clone EPR3208 Rabbit Monoclonal - 2453125 2453125
      Anti-MCAM [CD146], clone EPR3208 Rabbit Monoclonal 2470337
      Anti-MCAM [CD146], clone EPR3208 Rabbit Monoclonal - 2051948 2051948
      Anti-MCAM [CD146], clone EPR3208 Rabbit Monoclonal - 2204938 2204938
      Anti-MCAM [CD146], clone EPR3208 Rabbit Monoclonal - 2224465 2224465
      Anti-MCAM [CD146], clone EPR3208 Rabbit Monoclonal - 2297283 2297283
      Anti-MCAM [CD146], clone EPR3208 Rabbit Monoclonal - 2558380 2558380
      Anti-MCAM [CD146], clone EPR3208 Rabbit Monoclonal - JBC1793820 JBC1793820
      Anti-MCAM [CD146], clone EPR3208 Rabbit Monoclonal - NG1689932 NG1689932
      Anti-MCAM [CD146], clone EPR3208 Rabbit Monoclonal - NG1740989 NG1740989
      Anti-MCAM [CD146], clone EPR3208 Rabbit Monoclonal - NG1820800 NG1820800
      Anti-MCAM [CD146], clone EPR3208 Rabbit Monoclonal - NG1845594 NG1845594
      Anti-MCAM [CD146], clone EPR3208 Rabbit Monoclonal - NG1906435 NG1906435
      Anti-MCAM [CD146], clone EPR3208 Rabbit Monoclonal -2488799 2488799


      Reference overviewPub Med ID
      Embryonic rat vascular smooth muscle cells revisited - a model for neonatal, neointimal SMC or differentiated vascular stem cells?
      Kennedy, E; Hakimjavadi, R; Greene, C; Mooney, CJ; Fitzpatrick, E; Collins, LE; Loscher, CE; Guha, S; Morrow, D; Redmond, EM; Cahill, PA
      Vascular cell 6 6 2014

      Show Abstract
      24628920 24628920
      The role of endothelial cells in myofiber differentiation and the vascularization and innervation of bioengineered muscle tissue in vivo.
      Criswell, TL; Corona, BT; Wang, Z; Zhou, Y; Niu, G; Xu, Y; Christ, GJ; Soker, S
      Biomaterials 34 140-9 2013

      Show Abstract
      23059002 23059002

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