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ABE293 | Anti-MBD3 Antibody

ABE293
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, ICC, IHC, IP Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberABE293
      DescriptionAnti-MBD3 Antibody
      Alternate Names
      • Methyl-CpG-binding domain protein 3
      • Methyl-CpG-binding protein MBD3
      Background InformationDNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, regulates gene expression and is critical for proper development. Human proteins MBD3, MECP2, MBD1, MBD2, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD3 is a component of the Nucleosome Remodeling and Histone Deacetylation (NuRD) complex, which functions as a co-repressor, mediating gene silencing under different cellular contexts. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. MBD1 has been shown to play a role in chromatin modification through interaction with the histone H3K9 methyltransferase SETDB1. MBD1, MBD2, MBD3, and MBD4 are expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa nuclear extract
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-MBD3 Antibody is an antibody against MBD3 for use in Western Blotting, ICC, IHC, Immunoprecipitation.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated MBD3 from HeLa RIPA lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human MBD3.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes both isoforms of MBD3.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Mouse, Rat, and Rhesus Macaque based on 100% sequence homology. Other homologies: Bovine (83% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MBD3
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~32-34 kDa observed. Uniprot describes two isoforms at ~29 and ~33 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa nuclear extract.

      Western Blot Analysis: 0.2 µg/mL of this antibody detected MBD3 in 10 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies