MAB378 Anti-MAP2A Antibody, 2B

MAB378
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Am, Av, B, H, M, R, Xn, Qu IHC, WB M Ascites Monoclonal Antibody
      Description
      Catalogue NumberMAB378
      ReplacesMAB3418
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-MAP2A Antibody, 2B
      Alternate Names
      • Microtubule Associated Protein 2
      Background InformationMAP-2 (microtubule associated protein-2) is on of several high molecular weight proteins that play an important role in brain microtubule assembly. In addition to its association with microtubules, MAP-2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles (Binder, 1984).

      MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2 (Caceres, 1984; Binder, 1986). MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds (Caceres, 1986; Dotti, 1987).
      References
      Product Information
      FormatAscites
      Control
      • Brain tissue
      • Cultured neurons
      PresentationUnpurified mouse monoclonal IgG1 ascites fluid in buffer containing 15 mM sodium azide
      Applications
      ApplicationDetect MAP2A using this Anti-MAP2A Antibody, 2B validated for use in IH & WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry:
      1:50-1:200. AP20 stains dendrites and neuron cells bodies but not neuronal processes and axons. A previous lot of this antibody was used in immunohistochemistry.

      Western blot:
      SDS-PAGE Profiles: In SDS-PAGE MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 300 kD. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-2b). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier).

      Western blot: A 1:500 dilution was used in a previous lot.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenBovine MAP2
      CloneAP20
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityMAP2a & b. Does not recognize other variants.
      IsotypeIgG1
      Species Reactivity
      • Amphibian
      • Avian
      • Bovine
      • Human
      • Mouse
      • Rat
      • Xenopus
      • Quail
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isforms have been described.
      Gene Symbol
      • MAP2
      • MAP2B
      • MAP-2
      • MAP2C
      • DKFZp686I2148
      • MAP2A
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P11137 # The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
      SIZE: 1827 amino acids; 199539 Da
      PTM: MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
      SIMILARITY: SwissProt: P11137 ## Contains 3 Tau/MAP repeats.
      Molecular Weight~ 300 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on PC12 lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected MAP 2 on 10 μg of PC12 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC in undiluted aliquots from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-MAP2A, 2B - 23706932370693
      Anti-MAP2A, 2B - 24556972455697
      Anti-MAP2A, 2B - 20909342090934
      Anti-MAP2A, 2B - 21596502159650
      Anti-MAP2A, 2B - 22956652295665
      Anti-MAP2A, 2B - LV1486527LV1486527
      Anti-MAP2A, 2B - LV1583055LV1583055
      Anti-MAP2A, 2B - LV1657471LV1657471
      Anti-MAP2A, 2B - NG1865600NG1865600
      Anti-MAP2A, 2B -25006812500681

      References

      Reference overviewApplicationSpeciesPub Med ID
      HIV-1 Tat alters neuronal autophagy by modulating autophagosome fusion to the lysosome: implications for HIV-associated neurocognitive disorders.
      Fields, J; Dumaop, W; Eleuteri, S; Elueteri, S; Campos, S; Serger, E; Trejo, M; Kosberg, K; Adame, A; Spencer, B; Rockenstein, E; He, JJ; Masliah, E
      The Journal of neuroscience : the official journal of the Society for Neuroscience  35  1921-38  2015

      Show Abstract
      25653352 25653352
      STIM2 protects hippocampal mushroom spines from amyloid synaptotoxicity.
      Popugaeva, E; Pchitskaya, E; Speshilova, A; Alexandrov, S; Zhang, H; Vlasova, O; Bezprozvanny, I
      Molecular neurodegeneration  10  37  2015

      Show Abstract
      26275606 26275606
      SDF1 reduces interneuron leading process branching through dual regulation of actin and microtubules.
      Lysko, DE; Putt, M; Golden, JA
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  4941-62  2014

      Show Abstract
      24695713 24695713
      Ischemia induces different levels of hypoxia inducible factor-1α protein expression in interneurons and pyramidal neurons.
      Ramamoorthy, P; Shi, H
      Acta neuropathologica communications  2  51  2014

      Show Abstract
      Immunofluorescence24887017 24887017
      MHC class I limits hippocampal synapse density by inhibiting neuronal insulin receptor signaling.
      Dixon-Salazar, TJ; Fourgeaud, L; Tyler, CM; Poole, JR; Park, JJ; Boulanger, LM
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  11844-56  2014

      Show Abstract
      25164678 25164678
      Neurotrophins regulate ApoER2 proteolysis through activation of the Trk signaling pathway.
      Larios, JA; Jausoro, I; Benitez, ML; Bronfman, FC; Marzolo, MP
      BMC neuroscience  15  108  2014

      Show Abstract
      25233900 25233900
      Olfactory deficits in Niemann-Pick type C1 (NPC1) disease.
      Hovakimyan, M; Meyer, A; Lukas, J; Luo, J; Gudziol, V; Hummel, T; Rolfs, A; Wree, A; Witt, M
      PloS one  8  e82216  2013

      Show Abstract
      24391715 24391715
      Automated imaging system for fast quantitation of neurons, cell morphology and neurite morphometry in vivo and in vitro.
      Tapias, V; Greenamyre, JT; Watkins, SC
      Neurobiology of disease  54  158-68  2013

      Show Abstract
      Immunohistochemistry23220621 23220621
      Sorafenib selectively depletes human glioblastoma tumor-initiating cells from primary cultures.
      Carra, E; Barbieri, F; Marubbi, D; Pattarozzi, A; Favoni, RE; Florio, T; Daga, A
      Cell cycle (Georgetown, Tex.)  12  491-500  2013

      Show Abstract
      23324350 23324350
      Differential expression and HIV-1 regulation of μ-opioid receptor splice variants across human central nervous system cell types.
      Dever, SM; Xu, R; Fitting, S; Knapp, PE; Hauser, KF
      Journal of neurovirology  18  181-90  2012

      Show Abstract
      22528479 22528479

      Data Sheet

      Title
      Anti-MAP2A, 2B - Data Sheet

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies