MAB3418 Anti-MAP2 Antibody, clone AP20

MAB3418
200 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Ch, H, M, R, Xn, Qu IHC, WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB3418
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-MAP2 Antibody, clone AP20
      Alternate Names
      • Microtubule Associated Protein
      • MAP2A and 2B
      Background InformationMAP-2 (microtubule associated protein-2) is one of several high molecular weight proteins that play an important role in brain neuron microtubule assembly. In addition to its association with microtubules, MAP-2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles. MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2. MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds. (Caceres, 1986; Dotti, 1987).
      References
      Product Information
      FormatPurified
      Control
      • Brain, Neuronal culture, Human glioblastoma T98G cells.
      PresentationPurified mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate buffer, pH 7.6, 0.25 M NaCl with 0.1% sodium azide.
      Applications
      ApplicationAnti-MAP2 Antibody, clone AP20 detects level of MAP2 & has been published & validated for use in IH & WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern Blot:
      60 ng/mL. In SDS-PAGE, MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 280-300 kD representing the MAP-2A and -2B isoforms but not the 70 kDa MAP-2C isoform. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-2b). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier). Users should run 4%-20% SDS-PAGE gradient gels.

      Immunohistochemistry:
      5 µg/mL (4% PFA fixed frozen sections).

      Immunohistochemistry:
      5 µg/mL of a previous lot was used. Anti-MAP-2 can be used to stain tissue (brain or spinal cord) fixed with paraformaldehyde.

      Optimal working concentrations must be determined by the end user.
      Biological Information
      ImmunogenBovine brain microtubule protein.
      CloneAP20
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityMAB3418 binds specifically to MAP-2.
      IsotypeIgG
      Species Reactivity
      • Bovine
      • Chicken
      • Human
      • Mouse
      • Rat
      • Xenopus
      • Quail
      Species Reactivity NoteRat and mouse. Expected to cross-react with human, cow, chicken, quail, and Xenopus.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isforms have been described.
      Gene Symbol
      • MAP2
      • MAP2B
      • MAP-2
      • MAP2C
      • DKFZp686I2148
      • MAP2A
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P11137 # The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
      SIZE: 1827 amino acids; 199539 Da
      PTM: MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
      SIMILARITY: SwissProt: P11137 ## Contains 3 Tau/MAP repeats.
      Molecular Weight230 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Rat Brain lysate.

      Western Blot Analysis:
      1:1000 dilution of this lot detected MAP2 on 10 μg of Rat Brain lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
      During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-MAP2, clone AP203064831
      Anti-MAP2, clone AP20 - 21507222150722
      Anti-MAP2, clone AP20 - 24524942452494
      Anti-MAP2, clone AP20 - 19937751993775
      Anti-MAP2, clone AP20 - 20199582019958
      Anti-MAP2, clone AP20 - 20411052041105
      Anti-MAP2, clone AP20 - 20623122062312
      Anti-MAP2, clone AP20 - 22763432276343
      Anti-MAP2, clone AP20 - 23325532332553
      Anti-MAP2, clone AP20 - 24890652489065

      References

      Reference overviewApplicationSpeciesPub Med ID
      Differential regulation of apical-basolateral dendrite outgrowth by activity in hippocampal neurons.
      Yuan, Y; Seong, E; Yuan, L; Singh, D; Arikkath, J
      Frontiers in cellular neuroscience  9  314  2015

      Show Abstract
      26321915 26321915
      Human Induced Pluripotent Stem Cell Derived Neuronal Cells Cultured on Chemically-Defined Hydrogels for Sensitive In Vitro Detection of Botulinum Neurotoxin.
      Pellett, S; Schwartz, MP; Tepp, WH; Josephson, R; Scherf, JM; Pier, CL; Thomson, JA; Murphy, WL; Johnson, EA
      Scientific reports  5  14566  2015

      Show Abstract
      Immunoblotting (Western)26411797 26411797
      Crmp4 deletion promotes recovery from spinal cord injury by neuroprotection and limited scar formation.
      Nagai, J; Kitamura, Y; Owada, K; Yamashita, N; Takei, K; Goshima, Y; Ohshima, T
      Scientific reports  5  8269  2015

      Show Abstract
      Immunofluorescence25652774 25652774
      The HIF-1/glial TIM-3 axis controls inflammation-associated brain damage under hypoxia.
      Koh, HS; Chang, CY; Jeon, SB; Yoon, HJ; Ahn, YH; Kim, HS; Kim, IH; Jeon, SH; Johnson, RS; Park, EJ
      Nature communications  6  6340  2015

      Show Abstract
      Western Blotting25790768 25790768
      Heterogeneous calretinin expression in the avian cochlear nucleus angularis.
      Bloom, S; Williams, A; MacLeod, KM
      Journal of the Association for Research in Otolaryngology : JARO  15  603-20  2014

      Show Abstract
      24752525 24752525
      Cell-autonomous correction of ring chromosomes in human induced pluripotent stem cells.
      Bershteyn, M; Hayashi, Y; Desachy, G; Hsiao, EC; Sami, S; Tsang, KM; Weiss, LA; Kriegstein, AR; Yamanaka, S; Wynshaw-Boris, A
      Nature  507  99-103  2014

      Show Abstract
      Immunofluorescence24413397 24413397
      An inherited duplication at the gene p21 Protein-Activated Kinase 7 (PAK7) is a risk factor for psychosis.
      Morris, DW; Pearson, RD; Cormican, P; Kenny, EM; O'Dushlaine, CT; Perreault, LP; Giannoulatou, E; Tropea, D; Maher, BS; Wormley, B; Kelleher, E; Fahey, C; Molinos, I; Bellini, S; Pirinen, M; Strange, A; Freeman, C; Thiselton, DL; Elves, RL; Regan, R; Ennis, S; Dinan, TG; McDonald, C; Murphy, KC; O'Callaghan, E; Waddington, JL; Walsh, D; O'Donovan, M; Grozeva, D; Craddock, N; Stone, J; Scolnick, E; Purcell, S; Sklar, P; Coe, B; Eichler, EE; Ophoff, R; Buizer, J; Szatkiewicz, J; Hultman, C; Sullivan, P; Gurling, H; Mcquillin, A; St Clair, D; Rees, E; Kirov, G; Walters, J; Blackwood, D; Johnstone, M; Donohoe, G; , ; O'Neill, FA; , ; Kendler, KS; Gill, M; Riley, BP; Spencer, CC; Corvin, A
      Human molecular genetics  23  3316-26  2014

      Show Abstract
      Western Blotting24474471 24474471
      Progranulin protects against amyloid β deposition and toxicity in Alzheimer's disease mouse models.
      Minami, SS; Min, SW; Krabbe, G; Wang, C; Zhou, Y; Asgarov, R; Li, Y; Martens, LH; Elia, LP; Ward, ME; Mucke, L; Farese, RV; Gan, L
      Nature medicine  20  1157-64  2014

      Show Abstract
      25261995 25261995
      C9ORF72, implicated in amytrophic lateral sclerosis and frontotemporal dementia, regulates endosomal trafficking.
      Farg, MA; Sundaramoorthy, V; Sultana, JM; Yang, S; Atkinson, RA; Levina, V; Halloran, MA; Gleeson, PA; Blair, IP; Soo, KY; King, AE; Atkin, JD
      Human molecular genetics  23  3579-95  2014

      Show Abstract
      24549040 24549040
      Spatiotemporal changes in Cx30 and Cx43 expression during neuronal differentiation of P19 EC and NT2/D1 cells.
      Wan, CK; O'Carroll, SJ; Kim, SL; Green, CR; Nicholson, LF
      Cell biology international reports  20  13-23  2013

      Show Abstract
      25505515 25505515

      Technical Info

      Title
      Derivation of oligodendrocyte progenitor cells from a human neural stem cell line

      Data Sheet

      Title
      Anti-MAP2, clone AP20 - Data Sheet

      Newsletters / Publications

      Title
      Cellutions - The newsletter for Cell Biology Researchers Volume 3: 2011

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