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MABF19 | Anti-Ly1.2 (CD5) Antibody, clone 2145

MABF19
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M FC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABF19
      DescriptionAnti-Ly1.2 (CD5) Antibody, clone 2145
      Alternate Names
      • CD5 molecule
      • CD5 antigen (p56-62)
      • CD5 antigen
      • Lymphocyte antigen T1/Leu-1
      Background InformationCD5 (Ly1.2 in mouse) is a 55 kDa T-lymphocyte single chain transmembrane glycoprotein. It is present on all mature T-lymphocytes, on most thymocytes and on many T-cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T-lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T-cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B cell receptors.
      References
      Product Information
      FormatPurified
      Control
      • Mouse thymocytes
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationDetect Ly1.2 (CD5) using this Anti-Ly1.2 (CD5) Antibody, clone 2145 validated for use in FC.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenBalb/c thymus cells corresponding to B6 Ly-1.1 mouse CD5.
      EpitopeUnknown
      Clone2145
      HostMouse
      SpecificityThis antibody recognizes Ly1.2 (CD5).
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CD5
      • LEU1
      • T1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.

      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

      SEQUENCE SIMILARITIES; Contains 3 SRCR domains.
      Molecular Weight~53 kDa (Calculated)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in mouse thymocytes.

      Flow Cytometry Analysis: A 1:5,000 dilution of this antibody detected CD5 in mouse thymocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-Ly1.2 (CD5), clone 2145 - NRG1732249 NRG1732249

      References

      Reference overviewPub Med ID
      Preclinical evaluation of monoclonal antibody 14C5 for targeting pancreatic cancer.
      Liesbet Vervoort,Ingrid Burvenich,Steven Staelens,Caroline Dumolyn,Els Waegemans,Magali Van Steenkiste,Sarah K Baird,Andrew M Scott,Filip De Vos
      Cancer biotherapy & radiopharmaceuticals 25 2010

      Show Abstract
      20423233 20423233
      Fibroblast activation protein: purification, epitope mapping and induction by growth factors.
      W J Rettig,S L Su,S R Fortunato,M J Scanlan,B K Raj,P Garin-Chesa,J H Healey,L J Old
      International journal of cancer. Journal international du cancer 58 1994

      Show Abstract
      7519584 7519584
      Antibody targeting in metastatic colon cancer: a phase I study of monoclonal antibody F19 against a cell-surface protein of reactive tumor stromal fibroblasts.
      S Welt,C R Divgi,A M Scott,P Garin-Chesa,R D Finn,M Graham,E A Carswell,A Cohen,S M Larson,L J Old
      Journal of clinical oncology : official journal of the American Society of Clinical Oncology 12 1994

      Show Abstract
      8201382 8201382