A part of MilliporeSigma

06-719 | Anti-LexA Antibody, DNA-binding region

200 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      E. coli IP, WB Rb Purified Polyclonal Antibody
      Catalogue Number06-719
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-LexA Antibody, DNA-binding region
      Background InformationThe anti-LexA Antibody allows detection of LexA protein and recombinant proteins fused to the LexA protein. This antibody can be used to detect expression of potential bait proteins constructed for use with the yeast two-hybrid system or other interaction trap systems. LexA adds about 22 to 25 kDa to your protein, depending on the number of extra amino acids.
      Product Information
      • LexA-pRB transfected yeast cell extract
      PresentationProtein A Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide as a preservative.
      ApplicationDetect LexA with Anti-LexA Antibody, DNA-binding region (Rabbit Polyclonal Antibody), that has been shown to work in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenHis-tagged DNA binding domain of LexA protein corresponding to amino acid residues 1-202
      SpecificityRecognizes LexA portion of bait fusion proteins used in the yeast two-hybrid system.
      Species Reactivity
      • E. coli
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight24 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on LexA-pRB transfected yeast lysate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-LexA, DNA-binding region (rabbit polyclonal IgG) - 2121991 2121991
      Anti-LexA, DNA-binding region (rabbit polyclonal IgG) - 2462851 2462851
      Anti-LexA, DNA-binding region (rabbit polyclonal IgG) - 2201572 2201572
      Anti-LexA, DNA-binding region (rabbit polyclonal IgG) - 2377378 2377378
      Anti-LexA, DNA-binding region - 16359 16359
      Anti-LexA, DNA-binding region - 2018114 2018114
      Anti-LexA, DNA-binding region - 21691 21691
      Anti-LexA, DNA-binding region - 2557639 2557639
      Anti-LexA, DNA-binding region - 27842 27842
      Anti-LexA, DNA-binding region - DAM1513998 DAM1513998
      Anti-LexA, DNA-binding region - DAM1567237 DAM1567237
      Anti-LexA, DNA-binding region - DAM1588238 DAM1588238
      Anti-LexA, DNA-binding region - DAM1616395 DAM1616395
      Anti-LexA, DNA-binding region - JBC1381970 JBC1381970
      Anti-LexA, DNA-binding region - JBC1879873 JBC1879873
      Anti-LexA, DNA-binding region -2528717 2528717
      Anti-LexA, DNA-binding region -2654231 2654231
      Anti-LexA, DNA-binding region -2669863 2669863
      Anti-LexA, DNA-binding region -2727283 2727283
      Anti-LexA, DNA-binding region -2822043 2822043

      References | 12 Available | See All References

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      Western Blotting23975195 23975195
      PRAME is a golgi-targeted protein that associates with the Elongin BC complex and is upregulated by interferon-gamma and bacterial PAMPs.
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      Notch signaling is antagonized by SAO-1, a novel GYF-domain protein that interacts with the E3 ubiquitin ligase SEL-10 in Caenorhabditis elegans.
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      PloS one 6 e26109 2011

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      Insertional chromatin immunoprecipitation: a method for isolating specific genomic regions.
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      Lack of nuclear translocation of cytoplasmic domains of IL-2/IL-15 receptor subunits.
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      Genome-wide analysis of the functions of a conserved surface on the corepressor Tup1.
      Green, SR; Johnson, AD
      Molecular biology of the cell 16 2605-13 2005

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      Tyrosine residues direct the ubiquitination and degradation of the NY-1 hantavirus G1 cytoplasmic tail.
      Erika Geimonen, Imelyn Fernandez, Irina N Gavrilovskaya, Erich R Mackow
      Journal of virology 77 10760-868 2003

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