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MAB3536 | Anti-Lamin B2 Antibody, clone LN43

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Ht, M, Xn FC, WB, IHC M Purified Monoclonal Antibody
      Catalogue NumberMAB3536
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Lamin B2 Antibody, clone LN43
      Background InformationNuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished: A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.
      Product Information
      PresentationPurified culture supernatant. Liquid in PBS containing 0.09% sodium azide.
      ApplicationThis Anti-Lamin B2 Antibody, clone LN43 is validated for use in FC, WB, IH for the detection of Lamin B2.
      Key Applications
      • Flow Cytometry
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern blotting: 1:100-1:1,000; nuclear preparations recommended; detects 67-68kDa lamin B2 protein

      Immunocytochemistry on fixed cells (methanol/acetone fixation): 1:100-1:200.Flow cytometry: 1:100-1:200.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenDetergent insoluble fraction of potoroo cell line PtK1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityLamin B2. Recognizes an epitope located in the C-terminal part of lamin B2.
      Species Reactivity
      • Human
      • Hamster
      • Mouse
      • Xenopus
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B2. This gene is in a head-to-tail orientation with the gene for the translocase of inner mitochondrial membrane 13 homolog gene.
      Gene Symbol
      • LMNB2
      • Lamin-B2.
      • LMN2
      • MGC2721
      • LAMB2
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q03252 # Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
      SIZE: 600 amino acids; 67689 Da
      SUBCELLULAR LOCATION: Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side.
      PTM: B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
      DISEASE: SwissProt: Q03252 # Defects in LMNB2 are a cause of partial acquired lipodystrophy (APL) [MIM:608709]; also called Barraquer-Simons syndrome. APL is a rare childhood disease characterized by loss of subcutaneous fat from the face and trunk. Fat deposition on the pelvic girdle and lower limbs is normal or excessive. Most frequently, onset between 5 and 15 years of age. Most affected subjects are females and some show no other abnormality, but many develop glomerulonephritis, diabetes mellitus, hyperlipidaemia, and complement deficiency. Mental retardation in some cases. APL is a sporadic disorder of unknown aetiology.
      SIMILARITY: SwissProt: Q03252 ## Belongs to the intermediate filament family.
      MISCELLANEOUS: The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-LAMIN B2 -2733935 2733935
      MOUSE ANTI-LAMIN B2 -2772698 2772698


      Reference overviewPub Med ID
      Lamin B1 fluctuations have differential effects on cellular proliferation and senescence.
      Dreesen, O; Chojnowski, A; Ong, PF; Zhao, TY; Common, JE; Lunny, D; Lane, EB; Lee, SJ; Vardy, LA; Stewart, CL; Colman, A
      The Journal of cell biology 200 605-17 2013

      Show Abstract
      23439683 23439683
      A proteomic approach to identify candidate substrates of human adenovirus E4orf6-E1B55K and other viral cullin-based E3 ubiquitin ligases.
      Dallaire, F; Blanchette, P; Branton, PE
      Journal of virology 83 12172-84 2009

      Show Abstract Full Text Article
      19759146 19759146
      Coordinated regulation of cell cycle transcripts by p53-Inducible microRNAs, miR-192 and miR-215.
      Georges, SA; Biery, MC; Kim, SY; Schelter, JM; Guo, J; Chang, AN; Jackson, AL; Carleton, MO; Linsley, PS; Cleary, MA; Chau, BN
      Cancer research 68 10105-12 2008

      Show Abstract
      19074876 19074876
      Lamin proteins form an internal nucleoskeleton as well as a peripheral lamina in human cells.
      Hozák, P, et al.
      J. Cell. Sci., 108 ( Pt 2): 635-44 (1995) 1995

      Show Abstract
      7769007 7769007

      Data Sheet

      Anti-Lamin B2, clone LN43 - Data Sheet