MABC1152 | Anti-LINE-1 ORF1p Antibody, clone 4H1

MABC1152
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, ICC, IP, IH(P) M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABC1152
      DescriptionAnti-LINE-1 ORF1p Antibody, clone 4H1
      Alternate Names
      • LINE-1 retrotransposable element ORF1 protein
      • L1ORF1p
      • LINE retrotransposable element 1
      • LINE1 retrotransposable element 1
      Background InformationLINE-1 retrotransposable element ORF1 protein (UniProt: Q9UN81; also known L1ORF1p, LINE retrotransposable element 1, LINE1 retrotransposable element 1) is encoded by the LIRE1 (also known as LRE1) gene in human. LINE1-ORF 1P belongs to the transposase 22 family. It is mainly cytoplasmic protein and is enriched in stress granules, but may rarely be detected in the nucleolus. It contains a coiled coil domain that mediates its homotrimerization. The RNA recognition motif (RRM) the C-terminal domain are required for its proper RNA-binding activity. LINE1-ORF 1P is a nucleic acid-binding protein which is essential for retrotransposition of LINE-1 elements in the genome. It is also reported to function as a nucleic acid chaperone binding its own transcript and therefore preferentially mobilizing the transcript from which they are encoded. Retrotransposition is a shown to be a complex process that involves transcription of the LINE1, transport of its RNA to the cytoplasm, translation of the bicistronic RNA, formation of a ribonucleoprotein particle, its re-import to the nucleus and then target-primed reverse transcription at the integration site. Somatic LINE1 insertions are rare in most tissues, but are found in brain, testis, and many cancer types and have been implicated in carcinogenesis. Its expression is consistently detected in p53 mutant tumors, but, little or no expression is detected in Wilms tumors that were normal for p53. (Ref.: Goodier, JL et al. (2013). Nucleic Acids Res. 41(15): 7401-7419).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationDetect LINE-1 retrotransposable element ORF1 protein using this mouse monoclonal Anti-LINE-1 ORF1p Anibody, clone 4H1, Cat. No. MABC1152. It is used in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A representative lot detected LINE-1 ORF1p in Immunohistochemistry applications (Rodic, N., et. al. (2014). AM J Pathol. 184(5):1280-6; Doucet-O'Hare, T.T., et. al. (2015). Proc Natl Acad Sci USA. 112(35):E4894-900; Wylie, A., et. al. (2016). Genes Dev. 30(1):64-77).

      Immunocytochemistry Analysis: A representative lot detected LINE-1 ORF1p in Immunocytochemistry applications (Taylor, M.S., et. al. (2013). Cell 155(5):1034-48).

      Immunoprecipitation Analysis: A representative lot detected LINE-1 ORF1p in Immunoprecipitation applications (Taylor, M.S., et. al. (2013). Cell 155(5):1034-48).

      Western Blotting Analysis: A representative lot detected LINE-1 ORF1p in Western Blotting applications (Taylor, M.S., et. al. (2013). Cell 155(5):1034-48).
      Biological Information
      ImmunogenA linear peptide corresponding to 10 amino aicds from the N-terminal region of human LINE-1 ORF1 protein.
      Clone4H1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 4H1 specifically detects LINE-1 ORF1 protein in MCF7 cells. This clone targets the sequence corresponding to 10 amino acids from the N-terminal region of LINE-1 ORF1p.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • LIRE1
      • LRE1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~42 kDa observed; 40.06 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MCF7 cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LINE-1 ORF1p in 10 µg of MCF7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-LINE-1 ORF1p, clone 4H1 3069668
      Anti-LINE-1 ORF1p, clone 4H1 -Q2819727 Q2819727
      Anti-LINE-1 ORF1p, clone 4H1 Monoclonal Antibody 2880440
      Anti-LINE-1 ORF1p, clone 4H1 Monoclonal Antibody 2881932
      Anti-LINE-1 ORF1p, clone 4H1 Monoclonal Antibody 2919539
      Anti-LINE-1 ORF1p, clone 4H1 Monoclonal Antibody 2878047
      Anti-LINE-1 ORF1p, clone 4H1 Monoclonal Antibody 3009963
      Anti-LINE-1 ORF1p, clone 4H1 Monoclonal Antibody 2864347
      Anti-LINE-1 ORF1p, clone 4H1 Monoclonal Antibody 2911436