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MAB3751 | Anti-LEF-1 Antibody, β catenin binding domain, clone REMB1

250 µg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M IF, WB M Purified Monoclonal Antibody
      Catalogue NumberMAB3751
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-LEF-1 Antibody, β catenin binding domain, clone REMB1
      Alternate Names
      • Lymphoid Enhancer Factor 1
      Background InformationLymphoid Enhancer Factor -1 is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF-4). These factors play a crucial role in WNT/Wingless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer.LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. It is redundant with TCF-1 (for T Cell Factor-1) for correct development of T lymphocytes in the thymus. LEF-1 is a 399 amino acid protein that appears as a cluster of [polypeptides ranging in size between 55-57 kDa on SDS-PAGE analysis. Alternative splice forms of LEF-1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on westerns as smaller immunoreactive forms. Clone REMB1 recognizes the b-catenin binding domain of LEF-1.
      Product Information
      • Jurkat whole cell lysate, CCRF-HSB-2 (T lymphoblast) cell lysate, HuT 78 (cutaneous T lymphocyte) whole cell lysate
      PresentationPurified ascites. Provided as 0.2 μm sterile filtered solution in phosphate buffered saline with 0.08% sodiumazide.
      ApplicationDetect LEF-1 using this Anti-LEF-1 Antibody, β catenin binding domain, clone REMB1 validated for use in IF & WB.
      Key Applications
      • Immunofluorescence
      • Western Blotting
      Application NotesWestern blot (1-5 μg/ml)


      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenThe immunogen for the anti-LEF-1 antibody was a full length protein. Does not cross-react with TCF-1 or TCF-4.
      Epitopebeta catenin binding domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes the b-catenin binding domain of LEF-1(Lymphoid Enhancer Factor-1).
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryLymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905).[supplied by OMIM]
      Gene Symbol
      • LEF1
      • LEF-1
      • TCF1-alpha
      • TCF1ALPHA
      • DKFZp586H0919
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UJU2 # Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling.
      SIZE: 399 amino acids; 44201 Da
      SUBUNIT: Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with EP300, TLE1 and PIASG (By similarity). Binds THOC4, MDFI and MDFIC.
      SUBCELLULAR LOCATION: Nucleus (By similarity). Note=Found in nuclear bodies upon PIASG binding (By similarity).
      TISSUE SPECIFICITY: Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines.DOMAIN:SwissProt: Q9UJU2 Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
      SIMILARITY: Belongs to the TCF/LEF family. & Contains 1 HMG box DNA-binding domain.
      Stem Cell Type
      • Hematopoietic Stem Cells
      Molecular Weight50/57 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size250 µg
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies