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LP02 | Anti-LDL Receptor Mouse mAb (15C8)

LP02
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      B, H M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      LP02-100UG
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      Stocked 
      Discontinued
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      Available
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          Contact Customer Service

          Glass bottle 100 μg
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          Description
          OverviewRecognizes the ~160 kDa LDL receptor protein in HT1080 and HepG2 cells.
          Catalogue NumberLP02
          Brand Family Calbiochem®
          Application Data
          Detection of human LDL receptor by immunocytochemistry. Sample: HepG2 cells fixed in methanol. Primary antibody: Anti-LDL Receptor Mouse mAb (15C8) (Cat. No. LP02) (5 µg/ml). Detection: fluorescence.
          References
          Referencesvan Driel, I.R., et al. 1987. J. Biol. Chem. 262, 16127.
          van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 17443.
          Russell, D.W., et al. 1984. Cell 37, 577.
          Achneider, W.J., et al. 1983. J. Cell Biol. 97, 1635.
          Cummings, R.D., et al. 1983. J. Biol. Chem. 258, 15261.
          Schneider, W.J., et al. 1982 J. Biol. Chem. 257, 2664.
          Goldstein, J.L., et al. 1979. Nature 279, 679.
          Product Information
          FormLiquid
          FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
          Positive controlLiver sections, HT1080 cells, HepG2 cells
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesOriginal Clone Russell, D.W., et al. 1984. Cell 37, 577. Specificity van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 16127. Immunoblotting van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 17443. Flow Cytometry Duvillard, L., et al. 2003. Diabetes Care 26, 1540.
          Key Applications Enzyme-Linked Immunosorbent Assay
          Flow Cytometry
          Frozen Sections
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Immunoprecipitation
          Application NotesELISA (0.5-1 µg/ml)
          Flow Cytometry (see application references)
          Frozen Sections (1-5 µg/ml)
          Immunoblotting (1-5 µg/ml, see application references)
          Immunocytochemistry (1-5 µg/ml, IF)
          Immunoprecipitation (1-2 µg/sample)
          Application CommentsWill displace 50-70% of LDL binding to the receptor. For immunoblotting, include 2 mM Ca+2 with the antibody and avoid EDTA. This antibody has been reported to bind best to the unreduced LDL-R (see specificity application reference). Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena trypic digest of bovine low density lipoprotein receptor
          ImmunogenBovine
          Epitopefirst cysteine-rich ligand binding domain
          Clone15C8
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Bovine
          • Human
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          LP02

          References

          Reference overview
          van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 16127.
          van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 17443.
          Russell, D.W., et al. 1984. Cell 37, 577.
          Achneider, W.J., et al. 1983. J. Cell Biol. 97, 1635.
          Cummings, R.D., et al. 1983. J. Biol. Chem. 258, 15261.
          Schneider, W.J., et al. 1982 J. Biol. Chem. 257, 2664.
          Goldstein, J.L., et al. 1979. Nature 279, 679.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision12-June-2008 JSW
          ApplicationELISA (0.5-1 µg/ml)
          Flow Cytometry (see application references)
          Frozen Sections (1-5 µg/ml)
          Immunoblotting (1-5 µg/ml, see application references)
          Immunocytochemistry (1-5 µg/ml, IF)
          Immunoprecipitation (1-2 µg/sample)
          Application Data
          Detection of human LDL receptor by immunocytochemistry. Sample: HepG2 cells fixed in methanol. Primary antibody: Anti-LDL Receptor Mouse mAb (15C8) (Cat. No. LP02) (5 µg/ml). Detection: fluorescence.
          DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0-Ag14 myeloma cells (see application references). Recognizes the ~160 kDa LDL Receptor protein.
          BackgroundThe low density lipoprotein receptor is responsible for mediating the uptake of LDL, a cholesterol carrying plasma lipoprotein. The receptor is a member of a class of cell surface proteins which are concentrated in clathrin coated pits and carry external ligands into cells by receptor-mediated endocytosis. The purified receptor is a glycoprotein with a molecular weight of 160,000. It is synthesized as a precursor of molecular weight 120,000 that is then glycosylated to the mature form by addition of N-linked and O-linked sugars in the Golgi apparatus. Unlike other hepatic lectins, the orientation of the LDL-R is such that the amino terminus is to the exterior of the cell. It has recently been determined that the LDL-R has sequence homology with the epidermal growth factor receptor precursor.
          HostMouse
          Immunogen speciesBovine
          Immunogena trypic digest of bovine low density lipoprotein receptor
          Epitopefirst cysteine-rich ligand binding domain
          Clone15C8
          IsotypeIgG2a
          Speciesbovine, human, not rabbit
          Positive controlLiver sections, HT1080 cells, HepG2 cells
          FormLiquid
          FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsWill displace 50-70% of LDL binding to the receptor. For immunoblotting, include 2 mM Ca+2 with the antibody and avoid EDTA. This antibody has been reported to bind best to the unreduced LDL-R (see specificity application reference). Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          Referencesvan Driel, I.R., et al. 1987. J. Biol. Chem. 262, 16127.
          van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 17443.
          Russell, D.W., et al. 1984. Cell 37, 577.
          Achneider, W.J., et al. 1983. J. Cell Biol. 97, 1635.
          Cummings, R.D., et al. 1983. J. Biol. Chem. 258, 15261.
          Schneider, W.J., et al. 1982 J. Biol. Chem. 257, 2664.
          Goldstein, J.L., et al. 1979. Nature 279, 679.
          Application referencesOriginal Clone Russell, D.W., et al. 1984. Cell 37, 577. Specificity van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 16127. Immunoblotting van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 17443. Flow Cytometry Duvillard, L., et al. 2003. Diabetes Care 26, 1540.