A part of MilliporeSigma

06-807 | Anti-LAT Antibody

400 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M IP, WB Rb Purified Polyclonal Antibody
      Catalogue Number06-807
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-LAT Antibody
      Product Information
      • Positive Control Included: Jurkat cell lysate (12-303)
      PresentationProtein A Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, and 0.05% sodium azide.
      ApplicationAnti-LAT Antibody detects level of LAT & has been published & validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenGST-tagged fusion protein corresponding to residues 31-233 of human LAT
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes both forms of LAT.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms.
      Gene Symbol
      • LAT
      • LAT1
      • pp36
      • p36-38
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O43561 # Required for TCR (T-cell antigen receptor)- and pre-TCR- mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
      SIZE: 262 amino acids; 27930 Da
      SUBUNIT: When phosphorylated, interacts directly with the PIK3R1 subunit of phosphoinositide 3-kinase and the SH2 domains of GRB2, GRAP, GRAP2, PLCG1 and PLCG2. Interacts indirectly with CBL, SOS, VAV, and LCP2. Interacts with SHB and SKAP2.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type III membrane protein. Note=Present in lipid rafts.
      TISSUE SPECIFICITY: Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
      PTM: Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. & Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.
      MISCELLANEOUS: Engagement of killer inhibitory receptors (KIR) disrupts the interaction of PLCG1 with LAT and blocks target cell- induced activation of PLC, maybe by inducing the dephosphorylation of LAT.
      Molecular Weight36/38 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from Jurkat cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size400 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-LAT 2470970
      Anti-LAT (rabbit polyclonal IgG) - 2189948 2189948
      Anti-LAT (rabbit polyclonal IgG) - 2227257 2227257
      Anti-LAT (rabbit polyclonal IgG) - 2243497 2243497
      Anti-LAT (rabbit polyclonal IgG) - 2322513 2322513
      Anti-LAT - 16842 16842
      Anti-LAT - 18080 18080
      Anti-LAT - 19158 19158
      Anti-LAT - 2043523 2043523
      Anti-LAT - 22622 22622
      Anti-LAT - DAM1522874 DAM1522874
      Anti-LAT -2590151 2590151
      Anti-LAT -2647459 2647459
      Anti-LAT -2664260 2664260
      Anti-LAT -2716182 2716182
      Anti-LAT -2779432 2779432

      References | 26 Available | See All References

      Reference overviewApplicationPub Med ID
      Quantitative proteomics analysis of signalosome dynamics in primary T cells identifies the surface receptor CD6 as a Lat adaptor-independent TCR signaling hub.
      Roncagalli, R; Hauri, S; Fiore, F; Liang, Y; Chen, Z; Sansoni, A; Kanduri, K; Joly, R; Malzac, A; Lähdesmäki, H; Lahesmaa, R; Yamasaki, S; Saito, T; Malissen, M; Aebersold, R; Gstaiger, M; Malissen, B
      Nature immunology 15 384-92 2014

      Show Abstract
      24584089 24584089
      Assessment of caspase mediated degradation of linker for activation of T cells (LAT) at a single cell level.
      Kłossowicz, Mikołaj, et al.
      J. Immunol. Methods, 389: 9-17 (2013) 2013

      Show Abstract
      23261919 23261919
      Complementary phosphorylation sites in the adaptor protein SLP-76 promote synergistic activation of natural killer cells.
      Kim, HS; Long, EO
      Science signaling 5 ra49 2012

      Show Abstract
      22786724 22786724
      Phospholipase Cγ2 plays a role in TCR signal transduction and T cell selection.
      Fu, G; Chen, Y; Schuman, J; Wang, D; Wen, R
      Journal of immunology (Baltimore, Md. : 1950) 189 2326-32 2012

      Show Abstract
      22837484 22837484
      OX40 complexes with phosphoinositide 3-kinase and protein kinase B (PKB) to augment TCR-dependent PKB signaling.
      So, T; Choi, H; Croft, M
      Journal of immunology (Baltimore, Md. : 1950) 186 3547-55 2011

      Show Abstract
      21289304 21289304
      Beta-catenin inhibits T cell activation by selective interference with linker for activation of T cells-phospholipase C-γ1 phosphorylation.
      Driessens, G; Zheng, Y; Locke, F; Cannon, JL; Gounari, F; Gajewski, TF
      Journal of immunology (Baltimore, Md. : 1950) 186 784-90 2011

      Show Abstract
      21149602 21149602
      β2 integrin induces TCRζ-Syk-phospholipase C-γ phosphorylation and paxillin-dependent granule polarization in human NK cells.
      March, ME; Long, EO
      Journal of immunology (Baltimore, Md. : 1950) 186 2998-3005 2011

      Show Abstract
      21270398 21270398
      T-cell receptor microclusters critical for T-cell activation are formed independently of lipid raft clustering.
      Hashimoto-Tane, A; Yokosuka, T; Ishihara, C; Sakuma, M; Kobayashi, W; Saito, T
      Molecular and cellular biology 30 3421-9 2010

      Show Abstract
      Western Blotting20498282 20498282
      Vitamin D controls T cell antigen receptor signaling and activation of human T cells.
      von Essen MR, Kongsbak M, Schjerling P, Olgaard K, Odum N, Geisler C
      Nat Immunol 11 344-9. Epub 2010 Mar 7. 2010

      Show Abstract
      20208539 20208539
      Targeting of the small GTPase Rap2b, but not Rap1b, to lipid rafts is promoted by palmitoylation at Cys176 and Cys177 and is required for efficient protein activation in human platelets.
      Ilaria Canobbio, Piera Trionfini, Gianni F Guidetti, Cesare Balduini, Mauro Torti, Ilaria Canobbio, Piera Trionfini, Gianni F Guidetti, Cesare Balduini, Mauro Torti
      Cellular signalling 20 1662-70 2008

      Show Abstract
      18582561 18582561

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      Included Positive Control

      Catalog Number Description  
      12-303 Jurkat Cell Lysate Show Pricing & Availability

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      Life Science Research > Antibodies and Assays > Primary Antibodies