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05-659 | Anti-Kaiso Antibody, clone 6F

200 µg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ca, Ch, H, Ht, M, Mk, R EMSA, IP, WB, IHC M Purified Monoclonal Antibody
      Catalogue Number05-659
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Kaiso Antibody, clone 6F
      Product Information
      Presentation0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide and 30% glycerol.
      ApplicationDetect Kaiso with Anti-Kaiso Antibody, clone 6F (Mouse Monoclonal Antibody), that has been shown to work in EMSA, IP, WB & IHC.
      Key Applications
      • Electrophoretic Mobility Shift Assay
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry
      Biological Information
      ImmunogenRecombinant N-terminal GST and His-tagged fusion protein corresponding to residues 103-584 of murine Kaiso
      Cloneclone 6F
      Species Reactivity
      • Canine
      • Chicken
      • Human
      • Hamster
      • Mouse
      • Monkey
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ZBTB33
      • ZNF348
      • kaiso
      • ZNF-kaiso
      • WUGSC:H_DJ525N14.1
      • KAISO
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q86T24 # Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2.
      SIZE: 672 amino acids; 74484 Da
      SUBUNIT: Self-associates. Interacts with CTNND2 (By similarity). Interacts with CTNND1, and this interaction inhibits binding to both methylated and non-methylated DNA. Interacts with NCOR1. Interacts with KPNA2/RCH1, which may mediate nuclear import of this protein.
      SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Also cytoplasmic in cells grown at high densities.
      TISSUE SPECIFICITY: Expressed in vascular endothelium.
      SIMILARITY: SwissProt: Q86T24 ## Contains 1 BTB (POZ) domain. & Contains 3 C2H2-type zinc fingers.
      Molecular Weight97kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceImmunoblot Analysis: 0.5-2 μg/ml of this antibody detected Kaiso in RIPA lysates from 3T3/A31cells.
      Immunohistochemistry: As shown by an independent laboratory, 1μg/ml of this antibody gave positive nuclear immunostaining for Kaiso in NIH 3T3 cells fixed with 100% methanol (Daniel, 2001; Daniel, 1999).
      Immunoprecipitation: As shown by an independent laboratory, 2-5μg of this antibody immunoprecipitated Kaiso from RIPA lysates from NIH 3T3 cells (Daniel, 1999; Prokhortchouk, 2001).
      Supershift: As shown by an independent laboratory, this antibody will supershift a specific methyl-CpG complex (Prokhortchouk, 2001).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Kaiso, clone 6F (mouse monoclonal IgG1) - 2424740 2424740
      Anti-Kaiso, clone 6F (mouse monoclonal IgG1) - 2280866 2280866
      Anti-Kaiso, clone 6F - 22225 22225
      Anti-Kaiso, clone 6F -2786081 2786081


      Reference overviewPub Med ID
      The p120 catenin partner Kaiso is a DNA methylation-dependent transcriptional repressor.
      Prokhortchouk, A, et al.
      Genes Dev., 15: 1613-8 (2001) 2001

      Show Abstract
      11445535 11445535
      Monoclonal antibodies to Kaiso: a novel transcription factor and p120ctn-binding protein.
      Daniel, J M, et al.
      Hybridoma, 20: 159-66 (2001) 2001

      Show Abstract
      11461664 11461664
      The catenin p120(ctn) interacts with Kaiso, a novel BTB/POZ domain zinc finger transcription factor.
      Daniel, J M and Reynolds, A B
      Mol. Cell. Biol., 19: 3614-23 (1999) 1999

      Show Abstract
      10207085 10207085

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      Catalog Number Description  
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      Life Science Research > Antibodies and Assays > Primary Antibodies