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ABT97 | Anti-KIF3B Antibody

100 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, ICC Rb Affinity Purified Polyclonal Antibody
      Catalogue NumberABT97
      DescriptionAnti-KIF3B Antibody
      Alternate Names
      • Kinesin-like protein KIF3B
      • HH0048
      • Microtubule plus end-directed kinesin motor 3B
      Background InformationKinesin-like protein KIF3B (KIF3B) tethers chromosomes to the spindle pole and is involved in chromosomal movement. It forms a heterotrimeric protein with KIF3A and kinesin-like associated protein (KAP3). Both KIF3A and KIF3B contain an N-terminal globular motor domain, a potential neck coiled-coil region, a highly charged hinge region, a coiled-coil stalk region, and a C-terminal tail (The C-terminus region binds to KAP3). KIF3B interacts directly with certain proteins, such as CLC-5, IFT20, and the SMC3 subunit of the cohesion complex. It was shown to facilitate CLC-5 plasma membrane expression, endocytosis and microtubular transport in patients with Dent’s disease.
      Product Information
      FormatAffinity Purified
      • Human testis tissue lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationDetect the KIF3B protein using this Anti-KIF3B validated for use in WB & ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected KIF3B during telophase in A431 cells and during cytokinesis in HeLa cells. Punctate cytoplasmic staining has been observed in KIF3B-GFP transfected HEK293 cells (Reed, A. A., et al. (2010). Am J Physiol Renal Physiol. 298(2):F365-380.).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human KIF3B.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Chicken, Bovine, Xenopus, Rat, Rhesus Macaque, and Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KIF3B
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~85 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human testis tissue lysate.

      Western Blot Analysis: 5 µg/mL of this antibody detected KIF3B in 10 µg of human testis tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies