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MABE150 | Anti-KDM5B Antibody, clone 15G8.1

100 μg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB, IH(P) M Purified Monoclonal Antibody
      Catalogue NumberMABE150
      DescriptionAnti-KDM5B Antibody, clone 15G8.1
      Alternate Names
      • Lysine-specific demethylase 5B
      • KDM5B
      • Cancer/testis antigen 31
      • CT31
      • Histone demethylase JARID1B
      • Jumonji/ARID domain-containing protein 1B
      • PLU-1
      • Retinoblastoma-binding protein 2 homolog 1
      • RBP2-H1
      Background InformationLysine-specific demethylase 5B (UniProt Q9UGL1; also known as Cancer/testis antigen 31, CT31, Histone demethylase JARID1B, Jumonji AT rich interactive domain 1B, Jumonji/ARID domain-containing protein 1B, PLU-1, Protein phosphatase 1 regulatory subunit 98, Putative DNA/chromatin binding motif, RBP2-H1, Retinoblastoma-binding protein 2 homolog 1) is encoded by the KDM5B (also known as PLU1, PUT1, JARID1B, PPP1R98, RBBP2H1, RBBP2H1A) gene (Gene ID 10765) in human. JARID1B/KDM5B is an H3K4me2/3 demethylase that regulates self-renewal and differentiation in a cell type-specific manner. JARID1B/KDM5B is highly expressed in embryonic stem cells (ESCs), hematopoietic stem cells (HSCs), and in various primary cancers including melanoma, breast, testicular, and is upregulated in leukemic cell lines.
      Product Information
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationThis Anti-KDM5B Antibody, clone 15G8.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of KDM5B.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected KDM5B in 10 µg of MCF-7 cell lysate.
      Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected KDM5B in human kidney and mouse cerebral cortex tissue.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the region between ARID and JmjC domain of human KDM5B.
      EpitopeRegion between ARID and JmjC domain
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityExpected to react with both spliced isoforms.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KDM5B
      • JARID1B
      • PLU1
      • RBBP2H1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~175 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected KDM5B in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies